Anti stat3 s727 pe clone 49 p stat3

Manufactured by BD

Anti-STAT3 (S727) PE (clone 49/P-STAT3) is a laboratory reagent used for the detection of phosphorylated STAT3 (S727) in flow cytometry applications. It is a phycoerythrin (PE)-conjugated monoclonal antibody that binds to the Ser727-phosphorylated form of the STAT3 transcription factor.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd45 apc cy7 clone 30 f11, by BioLegend (2 mentions) Phosflow kit, by BD (2 mentions) Anti stat3 y705 pe clone 4 p stat3, by BD (2 mentions) Anti pjak2 y1007 1008 alexa fluor 647 clone e132, by Abcam (2 mentions) Anti cd11b clone m1 70, by BD (1 mentions) Anti gr 1 clone rb6 8c5, by BD (1 mentions) Anti 1 a 1 e fitc clone m5 114.152, by BioLegend (1 mentions) Anti s100a8, by Thermo Fisher Scientific (1 mentions) Anti s100a8 clone e4f8v, by Cell Signaling Technology (1 mentions) Anti s100a9 clone d3u8m, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

STAT3 (29 citations) Anti-STAT3 (16 citations) Anti-p-STAT3-S727-PE (2 citations) Anti-p-STAT3-PE (2 citations)

2 protocols using anti stat3 s727 pe clone 49 p stat3

Multiparameter Flow Cytometry for Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell suspensions were surface labeled with fluorochrome-conjugated antibodies for 10 m at 4 °C in staining buffer (PBS containing 1% BSA). For phosphoprotein staining, cells were fixed and permeabilized using the Phosflow kit (BD) according to manufacturer’s protocol before staining for 1 h at room temperature. Data were acquired using LSRII (BD Biosciences) and analyzed using FlowJo software (Tree Star). The following antibodies were used for flow cytometry analysis: anti-CD45 APC/Cy7 (clone 30-F11; BioLegend), anti-Ly6G PE/ biotin (clone 1A8; BD, BioLegend), anti-CD11c PerCP (clone N418; BioLegend), anti-F4/80 PE (clone BM8; eBioscience), anti-I-A/I-E FITC (clone M5/114.152; BioLegend), anti-Gr1 (clone RB6–8C5; BD Pharmingen), anti-CD11b (clone M1/70; BD Pharmingen), anti-STAT3 (Y705) PE (clone 4/P-STAT3, BD Biosciences), anti-STAT3 (S727) PE (clone 49/P-STAT3, BD Biosciences), anti-pJAK2 (Y1007,1008) Alexa Fluor 647 (clone E132, abcam), anti-S100a8 (catalog PA5-86063, ThermoFisher Scientific).

Loh J.T., Lee K.G., Lee A.P., Teo J.K., Lim H.L., Kim S.S., Tan A.H, & Lam K.P. (2021). DOK3 maintains intestinal homeostasis by suppressing JAK2/STAT3 signaling and S100a8/9 production in neutrophils. Cell Death & Disease, 12(11), 1054.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry of Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell suspensions were surface labelled with fluorochrome-conjugated antibodies for 10 minutes at 4°C in staining buffer (PBS containing 1% BSA). For phosphoprotein staining, cells were fixed and permeabilized using the Phosflow kit (BD) according to manufacturer’s protocol before staining for 1 hour at room temperature. Data were acquired using LSRII (BD Biosciences) and analyzed using FlowJo software (Tree Star). The following antibodies were used for flow cytometry analysis: anti-CD45 APC/Cy7 (clone 30-F11; BioLegend), anti-Ly6G PE/biotin (clone 1A8; BD, BioLegend), anti-STAT3 (Y705) PE (clone 4/P-STAT3, BD Biosciences), anti-STAT3 (S727) PE (clone 49/P-STAT3, BD Biosciences), anti-pJAK2 (Y1007,1008) Alexa Fluor 647 (clone E132, abcam), anti-Erk1/2 (pT202/Yp204) PE (612593, BD Biosciences), anti-pIKKα/β (S176/180) PE (14938S, Cell Signaling Technology), anti-p-p38 (T180/Y182) FITC (612594, BD Biosciences), anti-S100a8 (clone E4F8V; Cell Signaling Technology), anti-S100a9 (clone D3U8M; Cell Signaling Technology), anti-pSHP2 (Y580) PE (MA5-28045, Invitrogen), goat anti-rabbit IgG (H+L) secondary antibody FITC (Invitrogen).

Loh J.T., Teo J.K, & Lam K.P. (2022). Dok3 restrains neutrophil production of calprotectin during TLR4 sensing of SARS-CoV-2 spike protein. Frontiers in Immunology, 13, 996637.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!