Log In Sign up
The largest database of trusted experimental protocols

Panc 1

Manufactured by Cytiva
Visit Supplier
Sourced in United States

PANC-1 is a cell line derived from a human pancreatic carcinoma. It is commonly used in cell culture and biomedical research.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Cytiva (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Panc 1, by American Type Culture Collection (3 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Cytiva (2 mentions) Bxpc 3, by Cytiva (2 mentions) Fetal bovine serum (fbs), by Beyotime (1 mentions) Cfpac, by American Type Culture Collection (1 mentions) Rpmi 1640 hyclone, by Cytiva (1 mentions) Hdlec, by ScienCell (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Endothelial cell medium (ecm), by ScienCell (1 mentions) Sw1990, by Merck Group (1 mentions) Mda mb 435, by American Type Culture Collection (1 mentions) Ht 29, by American Type Culture Collection (1 mentions) Colo205, by American Type Culture Collection (1 mentions) Rpmi 1640, by Cytiva (1 mentions) Mycoplasma detection kit, by American Type Culture Collection (1 mentions) Mia paca 2, by American Type Culture Collection (1 mentions)

7 protocols using panc 1

1

Xiaoji Recipe Effects on Pancreatic Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pancreatic cell lines (CFPAC (cat. no. CRL-1918; PANC1, cat. no. CRL-1469)) were provided by the ATCC (American Type Culture Collection, USA), CFPAC cell line was cultured in Iscove's modified Dulbecco's medium. PANC1 cell line was cultured in Dulbecco's modified Eagle's medium (DMEM, Cytiva, Shanghai, China). Both culture mediums were maintained in an incubator supplemented with g 10% FBS (Beyotime, Shanghai, China) at 37°C and 5% CO2. To evaluate the effects of Xiaoji recipe on cell malignant behaviors in vitro, the CFPAC and PANC1 cells were treated with 150 μg/ml or 300 μg/ml Xiaoji recipe.
Xia C., Chen D., Wang G., Sun H., Lin J., Chen C., Shen T., Cheng H., Pan C., Xu D., Yang H., Zhu Y, & Zhu H. (2022). Identification of Molecular Targets and Underlying Mechanisms of Xiaoji Recipe against Pancreatic Cancer Based on Network Pharmacology. Computational and Mathematical Methods in Medicine, 2022, 4640849.
+ Open protocol
+ Expand
2

Culturing Pancreatic and Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The PC cell lines PANC-1 and SW1990, immortalized human pancreatic ductal epithelial cells (HPDCs) and human lymphatic tube endothelial cells (HDLECs) were purchased from Guangzhou Genio Biotech Co., Ltd. The SW1990 and HPDC cells were cultured in DMEM (MilliporeSigma), the PANC-1 cells were cultured in RPMI-1640 (HyClone; Cytiva) and the HDLECs were cultured in Endothelial Cell Medium (ScienCell Research Laboratories, Inc.), each supplemented with 10% FBS (Thermo Fisher Scientific, Inc.) and 1% antibiotics (penicillin-streptomycin; Thermo Fisher Scientific, Inc.). Culture was performed under normoxic or hypoxic conditions in a humidified incubator at 37°C. The normoxic conditions were 20% O2, 5% CO2 and 75% N2, and the hypoxic conditions were 1% O2, 5% CO2 and 94% N2. Cells in the logarithmic growth stage were selected for subsequent experiments.
Hao S., Han W., Ji Y., Sun H., Shi H., Ma J., Yip J, & Ding Y. (2022). BANCR positively regulates the HIF-1α/VEGF-C/VEGFR-3 pathway in a hypoxic microenvironment to promote lymphangiogenesis in pancreatic cancer cells. Oncology Letters, 24(6), 422.
+ Open protocol
+ Expand
3

Characterization of Cell Line Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
RK13 (catalog no. CCL-37), Vero (catalog no. CCL-81), A549 (catalog no. CCL-185), PANC-1 (catalog no. CRL-1469), MDA-MB435 (catalog no. HTB-129), HT29 (catalog no. HTB-38), and Colo205 (catalog no. CCL-222) cells were purchased from the ATCC. Individual cell lines were tested for Mycoplasma contamination every month using a universal Mycoplasma detection kit (catalog no. 30-1012K) from ATCC. Cells were authenticated by examination of morphology and consistent in vitro proliferation. RK13, Vero, A549, PANC-1, and MDA-MB435 cells were cultured in DMEM (Cytiva) supplemented with 10% FBS (Gibco), 2 mmol/L glutamine (Invitrogen), and 100 μg of penicillin-streptomycin (P/S; Invitrogen). HT29 and Colo205 cells were cultured in McCoy's 5 (Cytiva) and RPMI1640 (Cytiva) media, respectively, supplemented with 10% FBS, 2 mmol/L glutamine, and 100 μg of P/S. All the cultures were maintained at 37°C in a humidified 5% incubator. Individual cryovials were thawed and cells were grown no more than 20 passages.
Rahman M.M., van Oosterom F., Enow J.A., Hossain M., Gutierrez-Jensen A.D., Cashen M., Everts A., Lowe K., Kilbourne J., Daggett-Vondras J., Karr T.L, & McFadden G. (2023). Nuclear Export Inhibitor Selinexor Enhances Oncolytic Myxoma Virus Therapy against Cancer. Cancer Research Communications, 3(6), 952-968.
+ Open protocol
+ Expand
4

Culturing Pancreatic Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The normal human pancreatic cell line HPDE and human pancreatic cancer cell line Capan-1 were obtained from Korea Research Institute of Bioscience and Biotechnology (KRIBB). The MiaPaCa-2, Panc-1, BxPC-3, AsPC-1, and CFPAC-1 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The HEK293T cell line was a gift from Professor Dae-Sik Lim of the Korea Advanced Institute of Science and Technology.
The HPDE cell line was cultured in the defined Keratinocyte SFM (K-SFM) medium (Gibco, CA, USA). Capan-1 and CFPAC-1 cell lines were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM, WELGENE, Daegu, Gyeongsangbuk-do, Korea). MiaPaCa-2 and Panc-1 cell lines were cultured in Dulbecco’s Minimal Essential Medium (DMEM, Cytiva, Marlborough, MA, USA) and BxPC-3 and AsPC-1 cell lines were maintained in RPMI-1640 (WELGENE). All mediums were supplemented with 10% fetal bovine serum (FBS, Cytiva) and 1% penicillin/streptomycin (Gibco) at 37 °C with 5% CO2.
Youn S.E., Jiang F., Won H.Y., Hong D.E., Kang T.H., Park Y.Y, & Koh S.S. (2022). PAUF Induces Migration of Human Pancreatic Cancer Cells Exclusively via the TLR4/MyD88/NF-κB Signaling Pathway. International Journal of Molecular Sciences, 23(19), 11414.
+ Open protocol
+ Expand
5

Culturing Diverse Pancreatic Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human pancreatic cancer cell lines BxPC3, SW1990, and PANC-1 were purchased from iCell Bioscience Inc (Shanghai, China). HS766T and colo357 cell lines were obtained from Shanghai Jining Shiye (Shanghai, China). PCI-35 cell was purchased from Hangzhou Young Eagle Biotechnology Co., Ltd (Hangzhou China). PANC-1, HS766T, and Colo357 cells were cultured in Dulbecco’s modified Eagle medium, while SW1990, BxPC3, and PCI-35 cells were grown in RPMI-1640 medium (HyClone Laboratories Inc., Waltham, Massachusetts, USA). All medium contained 10% fetal bovine serum (FBS, Zhejiang Tianhang Biotechnology Co., Ltd. Hangzhou, China), penicillin (100 U/ml), and streptomycin (100 µg/ml). Cells were maintained at 37°C in a humidified atmosphere of 5% CO2.
Liu X., Zhou Y., Peng J., Xie B., Shou Q, & Wang J. (2020). Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1α/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells. Frontiers in Pharmacology, 11, 495.
+ Open protocol
+ Expand
6

Culture of Human Pancreatic Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human pancreatic cancer cell lines PANC-1 and BxPC-3 were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). PANC-1 and BxPC-3 were cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone Laboratories Inc., Logan, UT, USA) and Roswell Park Memorial Institute (RPMI)-1640 medium (HyClone Laboratories Inc., Logan, UT, USA), respectively, and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, New York, NY, USA) and 1% (v/v) penicillin/streptomycin (P/S; Gibco, New York, NY, USA). Cultured cells were maintained at 37 °C with 5% CO2 in a humidified incubator.
Zhang M., He S., Ma X., Ye Y., Wang G., Zhuang J., Song Y, & Xia W. (2020). GINS2 affects cell viability, cell apoptosis, and cell cycle progression of pancreatic cancer cells via MAPK/ERK pathway. Journal of Cancer, 11(16), 4662-4670.
+ Open protocol
+ Expand
7

Cultivating Cancer Cell Lines for Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The hepatocellular carcinoma cell lines Hep G2 and Hep 3B, the colorectal carcinoma cell line HT-29, and the pancreatic adenocarcinoma cell line Panc-1 were obtained from the American Type Culture Collection (ATCC®, Manassas, VA, USA). Catalog number and passage number are provided in the supplementary material. Hep G2 and Hep 3B cell lines were cultured in Eagle's Minimal Essential Medium (EMEM) +2 mM L-Glutamine (Lonza, Walkersville, MD, USA), HT-29 in Mc Coy's 5A + 1.5 mM L-Glutamine, +2.2 g/L Sodium Bicarbonate and Panc-1 in Dulbecco's Modified Essential Medium (DMEM) + 4.5 g/L D-Glucose, +2 mM L-Glutamine, +110 mg/L Sodium Pyruvate (HyClone Laboratories, Logan, UT, USA). All medias were supplemented with 10% fetal calf serum (FCS) (HyClone Laboratories, Logan, UT, USA) and Penicillin/Streptomycin; 100 μg/ml each (Gibco, Grand Island, NY, USA). Cells were cultured in a humidified atmosphere of 5% CO2 at 37 °C. For testing, cells were seeded in black walled, clear bottom 96 well plates (1 × 104 cells per well; cytotoxicity, caspase 3/7 activity, reactive oxygen species & TUNEL assay) or in 24 well plates for radioactive experiments (7.5 × 104 cells per well) one day prior to treatment and testing.
Ludwig J.M., Gai Y., Sun L., Xiang G., Zeng D, & Kim H.S. (2016). SW43‐DOX ± loading onto drug‐eluting bead, a potential new targeted drug delivery platform for systemic and locoregional cancer treatment – An in vitro evaluation. Molecular Oncology, 10(7), 1133-1145.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!

  Request a quote for « Panc 1 »