Anti rabbit and anti mouse hrp conjugated secondary antibodies

3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP) (purity ≥99%) was obtained from Selleck (Shanghai, China) and dissolved in DMSO. PD184352 (CI-1040) was obtained from Selleck (Shanghai, China). Titanium (Ti) particles were purchased from Johnson Matthey Chemicals (MA, USA). Alpha modification of Eagle's medium (α-MEM), penicillin/streptomycin, and fetal bovine serum (FBS) were from HyClone (UT, USA). RANKL and M-CSF were from R&D Systems (MN, USA). Primary antibodies specific for phospho-NF-kB p65 (#3033), phospho-IkBα (#2859), NF-kB p65 (#8242), IkBα (#4814), phospho-ERK (#4370), phospho-p38 (#4511), phospho-JNK (#4668), ERK (#4695), p38 (#8690), and JNK (#9252) were from Cell Signaling Technology (MA, USA). MMP9 (ab76003) and NFATc1 (ab25916), CTSK (ab178647), c-fos (ab208942) were from Abcam (Cambridge, UK). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were from Multi Sciences (Shanghai, China).

PUN (purity ≥98%) was obtained from Sigma-Aldrich (St. Louis, USA) and dissolved in phosphate-buffered saline (PBS). PBS was used as the vehicle control. Recombinant RANKL was purchased from R&D Systems (Minneapolis, USA). Minimal essential medium (DMEM/high glucose) and fetal bovine serum (FBS) were purchased from HyClone (Logan, USA). LPS (E. coli 026: B6) was obtained from Sigma-Aldrich. Primary antibodies to phospho-NF-κB p65 (#3033), phospho-IκBα (#2859), NF-κB p65 (#8242), IκBα (#4814), phospho-ERK (#4370), phospho-p38 (#4511), phospho-JNK (#4668), ERK (#4695), p38 (#8690), and JNK (#9252) were purchased from Cell Signaling Technology (Boston, USA). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were obtained from Multi Sciences (Shanghai, China). A CCK-8 assay kit was purchased from ApexBio (Boston, USA).

Cinnamtannin B-1 [C₄₅H₃₆O₁₈; MW: 864.7; purity >95% (HPLC)] was obtained from Enzo Life Sciences, Inc. (New York, NY, USA), and was dissolved in DMSO (Sigma-Aldrich, USA). Recombinant RANKL and M-CSF were purchased from R&D Systems (Minneapolis, USA). Primary antibodies to phospho-NF-κB p65 (#3033), phospho-IκBα (#2859), NF-κB p65 (#8242), IκBα (#4814), NFATc1 (#8032S), and c-fos (#2250S) were purchased from Cell Signaling Technology (Boston, USA). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were obtained from Multi Sciences (Shanghai, China). A counting kit-8 (CCK-8) was purchased from ApexBio (Boston, USA).