The qualitative detection (IgG screening) and semi-quantitative (U/mL) detection of IgG class antibodies against SARS-CoV-2 were undertaken using the BioPlex 2200 SARS-CoV-2 IgG Panel (Bio-Rad Laboratories, Inc., Hercules, CA, USA). This device can screen and differentiate IgG antibodies to the receptor-binding domain (RBD) and the spike 1 (S1), spike 2 (S2), and nucleocapsid proteins (N) of the SARS-CoV-2 coronavirus [19 ,20 (link)]. The positive anti-SARS-CoV-2 N/RBD/S1/S2 IgG levels were set to ≥10 U/mL, particularly for levels that were above 100 U/mL, and the machine provided 1:16 dilutions of the anti-IgG levels. The machine’s upper limit of values obtained from the dilutions was set at 1600 U/mL. All of the calculations necessary to interpret the results were performed automatically by PlexTM 2200 System Software (Bio-Rad, USA) [19 ,20 (link),21 (link)].
Bioplex 2200 sars cov 2 igg panel
Manufactured by Bio-Rad
Sourced in United States
The BioPlex 2200 SARS-CoV-2 IgG Panel is a multiplex immunoassay designed to detect the presence of IgG antibodies against SARS-CoV-2 in human serum or plasma samples. The panel measures the antibody levels against multiple SARS-CoV-2 antigens simultaneously.
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6 protocols using bioplex 2200 sars cov 2 igg panel
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SARS-CoV-2 IgG Antibody Detection Assay
2
Multiplex SARS-CoV-2 IgG Antibody Panel
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The Multiplex addressable laser bead immunoassay BioPlex®2200 SARS-CoV-2 IgG Panel (Bio-Rad, Hercules, CA, USA) was used to detect the presence of IgG antibodies against the SARS-CoV-2 antigens in plasma: spike 1 (S1), spike 2 (S2), receptor-binding domain (RBD) and nucleocapsid (NCap). The manufacturer’s recommended cutoff was employed.
3
SARS-CoV-2 IgG Multiplex Immunoassay
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The BioPlex 2200 SARS-CoV-2 IgG Panel (BIO-RAD, Hercules, California, USA) is a multiplex immunoassay for the qualitative detection and semi-quantitative differentiation of IgG against the RBD, S1, S2 and N proteins of SARS-CoV-2. Results < 10U/mL are reported as negative and ≥ 10 U/mL as positive. The measuring range for the assay is 1–100 U/mL. Results outside of this range are reported as < 1 U/mL or > 100 U/mL.
4
SARS-CoV-2 Antibody Quantification
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IgG antibodies against the receptor-binding domain (RBD), spike 1 (S1), spike 2 (S2), and nucleocapsid (NCap) structural proteins of SARS-CoV-2 were quantified in paired samples of 126 COVID-19 patients using ALBIA technology with the BioPlex®2200 SARS-CoV-2 IgG Panel (Bio-Rad, Hercules CA, USA). The manufacturer’s recommended cutoff was used. Samples showing antibody levels above the detection limit of the system were normalized to the detection limit value.
5
SARS-CoV-2 Seroprevalence Among Hospital Staff
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From April 9 to May 30 of 2020, SARS-CoV–2 seroprevalence of CHUSJ professionals was evaluated. To this end, 1027 HCW were tested for the presence of anti-SARS-CoV–2 antibodies. Among these professionals, 720 were women and 307 men, with an average age of 40 and 33 years-old, respectively. The studied population was distributed by the following hospital departments: Emergency, Infectious Disease, Intensive Care, Clinical Pathology, Surgery, Ophthalmology, and Nephrology. Detection of anti-SARS-CoV–2 antibodies was performed using the following immunoassays: Euroimmun Anti-SARS-CoV–2 ELISA IgG (Euroimmun, Luebeck, Germany) (n = 519); Abbott SARS-CoV–2 IgG (Abbott Laboratories, Chicago, Illinois, EUA) (n = 455), and Elecsys Anti-SARS-CoV–2 Total (Roche Diagnostics, Basel, Switzerland) (n = 53). The BioPlex 2200 SARS-CoV–2 IgG Panel (BIO-RAD, Hercules, California, USA) was used as an alternative immunoassay for positive results confirmation. All tests were performed according to the manufacturer's instructions.
6
SARS-CoV-2 IgG Antibody Multiplex Assay
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The BioPlex 2200 SARS-CoV–2 IgG Panel (BIO-RAD, Hercules, California, USA) is a multiplex immunoassay for the qualitative detection and semi-quantitative differentiation of IgG class antibodies against the following targeted viral antigen: receptor-binding domain of SARS-CoV–2 spike protein, S1 domain of the SARS-CoV–2 spike protein, S2 domain of the SARS-CoV–2 spike protein and nucleocapsid (N) protein of SARS-CoV–2. SARS-CoV–2 IgG assay results are expressed as U/mL and as negative or positive. Results of <10U/mL are reported as negative and results of ≥10U/mL are reported as positive. The measuring range for the assays is 1–100 U/mL. Results outside of this range are reported as either <1or > 100 U/mL.
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