Cd45ra apc alexa efluor 780

Frozen UCB cells were thawed and selected using an Easysep Human CD34 positive selection kit and Easysep magnet (StemCell Technologies) according to the manufacturer's instructions. CD34+ cells were stained with the following antibodies: CD34 PerCP-Cy5.5 (BD Biosciences, 347203) and CD135 BV711 (BD Biosciences, 563908), CD38 PE-Cy7 (eBioscience, 25-0388-42), CD45RA APC-Alexa eFluor 780 (eBioscience, 47-0458-42), CD90 APC (eBioscience, 17-0909-42). Cells were sorted as followed: HSC CD34⁺CD38^-CD45RA^-CD90⁺, MPP CD34⁺CD38^-CD45RA^-CD90^-MLP CD34⁺CD38^-CD45RA⁺, CMP CD34⁺CD38⁺CD45RA^-CD135⁺, GMP CD34⁺CD38⁺CD45RA⁺CD135⁺ and MEP CD34⁺CD38⁺CD45RA^-CD135^-. DAPI was added to the cell suspension before sorting to exclude dead cells. Cells were sorted on a BD InfluxTM cell sorter operating in 6-way purity sort mode and collected into 1.5 ml microfuge tubes.