α actin antibody c4

Manufactured by MP Biomedicals

The α-actin antibody C4 is a monoclonal antibody that specifically recognizes the α-actin protein. It is a commonly used tool in cell and molecular biology research to detect and quantify the presence of α-actin in various cell and tissue samples.

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Lab products found in correlation

Odyssey detection system, by LI COR (1 mentions)

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α-actin

2 protocols using α actin antibody c4

Polyclonal Antibody Generation and Purification for RavK

Cited 2 times

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Polyclonal antibodies against RavK was generated at the Pocono Rabbit Farm and Laboratory using recombinant His₆-tagged RavKΔC50 purified from E. coli to immunize a rabbit. The RavK antibody was affinity purified following a standard protocol [53 (link)]. The α-actin antibody C4 was purchased from MP Biochemicals (0869100) and was used at 1:5,000. The α-ICDH, α-GFP, α-PGK, α-Flag, α-tubulin were used as described in an earlier study [48 (link)]. Signals from each individual protein were detected by fluorescence dye-conjugated antibodies on an Odyssey detection system (Li-Cor).

Liu Y., Zhu W., Tan Y., Nakayasu E.S., Staiger C.J, & Luo Z.Q. (2017). A Legionella Effector Disrupts Host Cytoskeletal Structure by Cleaving Actin. PLoS Pathogens, 13(1), e1006186.

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Antibody Validation for Protein Analysis

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The α-p53 (human, DO-1, sc-126) antibody was obtained from Santa Cruz Biotechnology. The α-p21 (12D1, #2947S), α-p53 (mouse, 1C12, #2524S) and α-Myc-tag (9B11, #2276) were purchased from Cell Signalling Technologies. The α-RPL5 (ab137617), α-RPL11 (ab79352), α-RPS18 (ab91293), α-RPL21 (ab215724), α-RPS19 (ab57643), α-RPL28 (ab193164) and α-RPL22 (ab77720) antibodies were procured from Abcam. The α-BrDU (B44, #347580) was obtained from BD Biosciences. The α-Actin antibody (C4, #691001) was acquired from MP Biomedicals, while the α-FLAG (F-3165) antibody was purchased from Merck Millipore. The goat α-mouse (#1706516) and α-rabbit (1706515) HRP-conjugated IgG (H+L) antibodies were obtained from BioRad. Alexa-Fluor 594 donkey α-mouse IgG (H+L, A21203) and Alexa-Fluor 488 goat α-mouse IgG (H+L, A11001) were purchased from ThermoFisher Scientific. Fluorescein isothiocyanate (FITC) conjugated sheep α-mouse IgG (Cappel, ICN Biomedical #55516) and horse anti-mouse IgG (FI-2000, Vector Labs) were also purchased.

Hannan K.M., Soo P., Wong M.S., Lee J.K., Hein N., Poh P., Wysoke K.D., Williams T.D., Montellese C., Smith L.K., Al-Obaidi S.J., Núñez-Villacís L., Pavy M., He J.S., Parsons K.M., Loring K.E., Morrison T., Diesch J., Burgio G., Ferreira R., Feng Z.P., Gould C.M., Madhamshettiwar P.B., Flygare J., Gonda T.J., Simpson K.J., Kutay U., Pearson R.B., Engel C., Watkins N.J., Hannan R.D, & George A.J. (2022). Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway. Cell Reports, 41(5), 111571.

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