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Automatic biochemistry analyzer

Manufactured by Abbott
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The Automatic Biochemistry Analyzer is a laboratory instrument designed to perform automated chemical analysis of biological samples. It is capable of measuring a variety of analytes, such as enzymes, proteins, and metabolites, in a high-throughput and efficient manner.

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Lab products found in correlation

Enzyme linked immunosorbent assay, by R&D Systems (1 mentions) Glucose analyzer, by Roche (1 mentions) Mouse albumin elisa kit, by Abcam (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions)

Close spelling variants of this product

Automatic analyzer (3 citations) ARCHITECT Automatic Biochemistry Analyzer (3 citations) Abbott c8000 Automatic Biochemistry Analyzer (2 citations)

9 protocols using automatic biochemistry analyzer

1

Cathepsin S in Coronary Atherosclerosis

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The level of cathepsin S was detected in all patients using enzyme-linked immunosorbent assay (R&D Systems, Inc.). The serum cathepsin S level and Gensini score were compared between the healthy subjects and patients with coronary atherosclerotic heart disease, and the correlation between serum cathepsin S level and Gensini score was analyzed. The carotid thickness, mean arterial pressure and indexes related to glucose and lipid metabolism, as well as vascular endothelial function were compared. Also, the correlation of the serum cathepsin S level with carotid intima-media thickness (IMT), mean arterial pressure, fasting blood glucose, total cholesterol (TC) and nitric oxide (NO) was investigated. Fasting blood glucose and TC were measured by an Automatic Biochemistry Analyzer (Abbott 8200; Abbott Pharmaceutical Co. Ltd.). NO was measured by a Nitric Oxide Colorimetric Assay kit (K262-200; AmyJet Scientific, Inc.).
Huang S, & Cao Y. (2019). Correlation of cathepsin S with coronary stenosis degree, carotid thickness, blood pressure, glucose and lipid metabolism and vascular endothelial function in atherosclerosis. Experimental and Therapeutic Medicine, 19(1), 61-66.
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2

Serum Biochemistry Analysis Protocol

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Serum was isolated by centrifugation at 1,811 x g for 5 min, and subsequently used for alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (TBIL) and direct bilirubin (DBIL) analyses using an automatic biochemistry analyzer (Abbott Laboratories, North Chicago, IL, USA), according to the manufacturer's protocol.
Hou F., Liu R., Liu X., Cui L., Wen Y., Yan S, & Yin C. (2016). Attenuation of liver fibrosis by herbal compound 861 via upregulation of BMP-7/Smad signaling in the bile duct ligation model rat. Molecular medicine reports, 13(5).
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3

Serum Metabolic Enzyme Evaluation

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The serum from mouse blood was isolated by centrifugation at 1,300 rpm for 10 min. The automatic biochemistry analyzer (Abbott Laboratories, USA) was used to evaluate the serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) according to the manufacturer’s protocol.
Lu F.B., Chen D.Z., Chen L., Hu E.D., Wu J.L., Li H., Gong Y.W., Lin Z., Wang X.D., Li J., Jin X.Y., Xu L.M, & Chen Y.P. (2019). Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p. Molecules and Cells, 42(12), 906-918.
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4

Serum Biomarker Quantification Protocol

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Blood samples were centrifuged at 4000×g for 5 min to separate the serum from other blood components. The samples were subsequently used to determine aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), and direct bilirubin (DBIL) levels using an automatic biochemistry analyzer (Abbott Laboratories, Chicago, IL, USA), according to the manufacturer’s protocol.
Chi C., Liu X.Y., Hou F., Yu X.Z., Li C.Y., Cui L.J., Liu R.X, & Yin C.H. (2018). Herbal compound 861 prevents hepatic fibrosis by inhibiting the TGF-β1/Smad/SnoN pathway in bile duct-ligated rats. BMC Complementary and Alternative Medicine, 18, 52.
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5

Serum Biomarker Evaluation Protocol

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The serum was isolated from blood samples of each group by 251.55 g centrifugation for 10 minutes. According to the manufacturer's protocol, the serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) were evaluated using an automatic biochemistry analyzer (Abbott Laboratories). Scrum TNF‐α levels were measured using mice ELISA kit (eBioscience). Scrum CHI3L1 levels were measured using mice CHI3L1 assay Kit (Hangzhou Proprium Biotech Company Ltd.). Scrum IgG levels were measured using mice ELISA kit (70‐EK271‐96, Multi Science (LIANKE) Biotech, Co. LTD). All experiments were according to the manufacturers’ instructions.
Tu H., Chen D., Cai C., Du Q., Lin H., Pan T., Sheng L., Xu Y., Teng T., Tu J., Lin Z., Wang X., Wang R., Xu L, & Chen Y. (2019). microRNA‐143‐3p attenuated development of hepatic fibrosis in autoimmune hepatitis through regulation of TAK1 phosphorylation. Journal of Cellular and Molecular Medicine, 24(2), 1256-1267.
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6

Comprehensive Murine Metabolic Profiling

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The urine of mice was collected from metabolic cages to measure 24 h proteinuria using mouse albumin ELISA kits (Abcam, USA). Animals were weighed and anesthetized with 30 mg/kg sodium pentobarbital (Sigma Aldrich) followed by cervical dislocation. The blood taken from the abdominal aorta was centrifugated at 3000 × g for 15 min to obtain the serum. An automatic biochemistry analyzer (Abbott Labs, IL, USA) was employed to measure serum creatinine and blood urea nitrogen levels. The blood taken from the tail vein was used for blood glucose measurement by a glucose analyzer (Roche). The left kidney was weighed to calculate the ratio of kidney weight to body weight.
Wang Y., Yang J., Wu C., Guo Y., Ding Y, & Zou X. (2024). LncRNA SNHG14 silencing attenuates the progression of diabetic nephropathy via the miR‐30e‐5p/SOX4 axis. Journal of Diabetes, 16(6), e13565.
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7

Serum Enzyme Evaluation in Anesthetized Animals

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When the animals were anesthetized with 10% chloral hydrate solution (3.5 mL/kg, i.p. injection), the blood samples from each group were collected and centrifuged at 4000 rpm at 4°C for 10 min. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using an automatic biochemistry analyzer (Abbott Laboratories, USA).
Chen S., Chen Y., Chen B., Cai Y.J., Zou Z.L., Wang J.G., Lin Z., Wang X.D., Fu L.Y., Hu Y.R., Chen Y.P, & Chen D.Z. (2015). Plumbagin Ameliorates CCl4-Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 645727.
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8

Serum Biomarker Quantification

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Individual serum samples were prepared by centrifugation at 1,811 × g for 5 min at 4°C, and the levels of serum aspartate transaminase (AST), alanine transaminase (ALT), direct bilirubin (DBIL) and total bilirubin (TBIL) were analyzed using an automatic biochemistry analyzer (Abbott Laboratories, Chicago, IL, USA), according to the manufacturer’s protocol.
Hou F., Liu R., Liu X., Cui L., Yu X., Wen Y., Ding H, & Yin C. (2017). Arkadia protein expression is reduced in the liver during the progression of hepatic fibrosis. International Journal of Molecular Medicine, 41(3), 1315-1322.
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9

Hepatic Injury Biomarker Evaluation

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Two ml blood was obtained from the inferior vena cava, frozen at -20 ℃ for 5 minutes and centrifuged for 15 minutes at 4,000 revolutions/min. Supernatants were transferred into fresh tubes for evaluation. The activities of alanine aminotransferase (ALT, a specific marker for hepatic parenchymal injury), and aspartate Aminotransferase (AST, a nonspecific marker for hepatic injury) in serum were determined in units per liter using automatic biochemistry analyzer (Abbott Laboratories, Abbott Park, IL, USA).
Liu J., Pan G., Liang T, & Huang P. (2014). HGF/c-Met Signaling Mediated Mesenchymal Stem Cell-induced Liver Recovery in Intestinal Ischemia Reperfusion Model. International Journal of Medical Sciences, 11(6), 626-633.
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