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Nicolet is5

Manufactured by Specac

The Nicolet iS5 is a Fourier Transform Infrared (FTIR) spectrometer designed for laboratory use. It provides accurate and reliable infrared spectroscopy measurements to identify and analyze a wide range of materials. The Nicolet iS5 features high-performance optics, a durable design, and user-friendly software, making it a versatile and efficient tool for various applications in research and analytical laboratories.

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5 protocols using nicolet is5

1

Solid Phase Infrared Spectroscopy

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Transmission infrared spectroscopy was performed on samples in the solid phase using a Thermo Scientific Nicolet iS5 instrument with Specac Golden Gate attachment and processed with OMNIC software (see Supplementary Figs 42 and 43 and Supplementary Table 5).
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2

FTIR Analysis of Lipopeptide Spectra

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The FTIR spectra of the lipopeptides
were recorded using a Thermo-Scientific Nicolet iS5 instrument with
a deuterated triglycine sulfate (DTGS) detector, with a Specac Pearl
liquid cell containing CaF2 plates, where the sample was
fixed. A total of 128 scans for each sample were recorded over the
range of 900–4000 cm–1.
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3

Infrared Spectroscopy of Aqueous Solutions

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Spectra were
recorded using a Thermo Scientific Nicolet iS5 instrument equipped
with a DTGS detector, with a Specac Pearl liquid cell (sample contained
between fixed CaF2 plates). Spectra (1 wt % sample in H2O) were scanned 128 times over the range of 900–4000
cm–1.
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4

IR Spectra Acquisition and Analysis

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IR spectra were acquired
on a Thermo Scientific Nicolet iS5 with a Specac Golden Gate Reflection
Diamond ATR System. All data were processed with a Thermo Scientific
OMNIC 8.3.103. All samples were measured in the native state at room
temperature (solid state, unless liquid at room temperature).
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5

ATR-FTIR Analysis of Colloidal Dispersions

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Attenuated total reflectance Fourier Transform infrared spectroscopy (ATR-FTIR) data for the colloidal dispersions (liquid phase) and the powders (solid-phase) were measured in the range of 500 to 4000 cm À1 using a Thermo Fisher Nicolet iS5 FTIR spectrometer equipped with a Specac Golden Gate Single Reflection Diamond ATR System, KBr optics, a diamond ATR crystal, and ZnSe lenses. Each spectrum was the average of 64 scans with a spectral resolution of 2 cm À1 . Data for each colloidal dispersion (liquid phase) were collected in situ by placing a drop of the dispersion on the ATR diamond, and using a cap to block light passing though the samples during measurements.
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