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2 protocols using tiliroside

1

Phenolic Compounds Quantification Protocol

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Ethanol and sodium carbonate were purchased from Avantor Performance Materials Poland (Gliwice, Poland). Gallic, m-coumaric, o-coumaric, p-coumaric, caffeic, 5-O-caffeoylqunic, ferulic, isoferulic, vanillic, salicylic, 3-hydroxybenzoic, 4-hydroxybenzoic, protocatechuic, syringic, rosmarinic acid, quercitrin, apigenin 7-O-glucoside, eriocitrin, taxifolin, 3-O-methylquercetin, isorhamnetin, isorhamnetin-3-O-glucoside, luteolin, kaempferol, rutin, hyperoside, isoquercetin, sakuranetin, aluminum chloride, and LC–MS grade acetonitrile were purchased from Sigma-Aldrich Chemical Co. (St. Louis, Mo, USA). Folin–Ciocalteu reagent was supplied by Chempur (Piekary Śląskie, Poland). Quercetin was purchased from Fluka (Buchs, Switzerland). Catechin, naringenin 7-O-glucoside, eriodictyol, luteolin-7-O-glucoside, naringenin 7-O-glucoside, luteolin 3,7-diglucoside, nicotiflorin, narcissoside, gentisic, sinapic acid, and myricetin were provided by ChromaDex (Irvine, CA, USA). Astragalin, apigenin, naringenin, kaempferol-3-rutinoside, and tiliroside were supplied by Roth (Karlsruhe, Germany). LC–MS grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA).
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2

Analytical Standards for Phytochemicals

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Analytical standards of apigetrin (apigenin 7-glucoside), chrysin, prunetin, rhamnazin, morin, isoquercetin, vitexin, isovitexin, quercitrin, kaempferitrin, taxifolin, luteolin, 3-O-methylquercetin, kaempferol, hyperoside, rutin, isorhamnetin, isokaempferide, rhamnetin, sakuranetin, luteoloside, eriodictyol-7-O-glucopyranoside, narirutin, naringin, LC grade acetonitryl, DMSO, DPPH radical, ampicillin, bovine serum, ferric chloride, hyaluronidase from bovine testes type I -S, sodium phosphate buffer pH 7, oleanolic acid, 2,4,6-tris(2-pyridyl)-s-triazine TPTZ, and hyaluronic acid were obtained from Sigma-Aldrich Fine Chemicals (St. Louis, MI, USA). Naringenin, astragalin, apigenin, tiliroside, and nicotiflorin were purchased from Roth (Karlsruhe, Germany). Dihydromyricetin was supplied by LGC (LGC Group, Teddington, UK), and laricitrin by Extrasynthese (Lyon, France). Quercetin was obtained from Fluka (Buchs, Switzerland). Catechin, luteolin-7-O-glucoside, luteolin 3,7-diglucoside, Naringenin 7-O-glucoside, eriodictyol, and myricetin were supplied by ChromaDex (Irvine, CA, USA). LC-MS grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). Ethanol was obtained from Avantor Performance Materials (Gliwice, Poland).
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