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The EZ-spot is a laboratory equipment product that serves as a spot blotting system. It is designed to efficiently transfer protein samples from a gel to a membrane for further analysis.

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Lab products found in correlation

Multiplexed fluorometric immunoassay, by Charles River Laboratories (2 mentions) Pcr rodent infectious agent array methods, by Charles River Laboratories (2 mentions) Serology profile assessment plus, by Charles River Laboratories (2 mentions)

5 protocols using ez spot

1

Comprehensive Murine Pathogen Screening

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Serum was collected at the indicated times for antibody screening using EZ-spot followed by a multiplexed fluorometric immunoassay (Charles River). The screening panel includes: mouse hepatitis virus (MHV), Sendai virus, pneumonia virus of mice, minute virus of mice (MVM), mouse parvovirus type 1(MPV), mouse parvovirus type 2, mouse parvovirus-NS1, murine norovirus (MNV), Theiler’s murine encephalomyelitis virus (TMEV), reovirus, rotavirus EDIM, lymphocytic choriomeningitis virus, ectromelia virus, mouse adenovirus 1 and 2, mouse cytomegalovirus, polyoma virus, Mycoplasma pulmonis, Enchephalitozoon cuniculi, cilia-associated respiratory bacillus, and Clostridium piliforme. Relative serology scores for MHV antigens (recombinant A59-strain nucleocapsid protein, purified A-59 viral lysate, and purified S-strain viral lysate) were calculated by Charles River using median fluorescence index. Active pathogen infection was measured by PCR Rodent Infectious Agent (PRIA) array methods (Charles River) in samples collected from oral swabs or fecal material. This panel screened for MHV, MNV, MPV, MVM, Rodentibacter heylii, and Helicobacter species.
Camell C.D., Yousefzadeh M.J., Zhu Y., Prata L.G., Huggins M.A., Pierson M., Zhang L., O’Kelly R.D., Pirtskhalava T., Xun P., Ejima K., Xue A., Tripathi U., Espindola-Netto J.M., Giorgadze N., Atkinson E.J., Inman C.L., Johnson K.O., Cholensky S.H., Carlson T.W., LeBrasseur N.K., Khosla S., O’Sullivan M.G., Allison D.B., Jameson S.C., Meves A., Li M., Prakash Y.S., Chiarella S.E., Hamilton S.E., Tchkonia T., Niedernhofer L.J., Kirkland J.L, & Robbins P.D. (2021). Senolytics reduce coronavirus-related mortality in old mice. Science (New York, N.y.), 373(6552), eabe4832.
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2

Comprehensive Rodent Pathogen Screening

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Serum was collected prior to and after 60 days of co-housing for serology and cytokine analysis. Serum was screened using EZ-spot and PCR Rodent Infectious Agent (PRIA) array methods (Charles River Laboratories) for sendai virus (SEND), pneumonia virus of mice (PVM), mouse hepatitis virus (MHV), Minute virus of mice (MVM), mouse parvovirus type 1 (MPV-1), mouse parvovirus type 2 (MPV-2), mouse parvovirus-NS1 (NS-1), murine norovirus (MNV), Theiler’s murine encephalomyelitis virus (GDVII), reovirus (REO), Rotavirus EDIM (EDIM/ROTA-A), lymphocytic choriomeningitis virus (LCMV), ectromelia virus (ECTRO), mouse adenovirus 1 and 2 (MAV1&2), mouse cytomegalovirus (MCMV), polyoma virus (POLY), mycoplasma pulmonis (MPUL), enchephalitozoon cuniculi (ECUN), cilia-associated respiratory bacillus (CARB), and clostridium piliforme (CPIL). Blood was also collected before and after co-housing to determine the composition and phenotype of circulating immune cells.
Huggins M.A., Sjaastad F.V., Pierson M., Kucaba T.A., Swanson W., Staley C., Weingarden A.R., Jensen I.J., Danahy D.B., Badovinac V.P., Jameson S.C., Vezys V., Masopust D., Khoruts A., Griffith T.S, & Hamilton S.E. (2019). Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell reports, 28(7), 1729-1743.e5.
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3

Pathogen Screening in Mouse Models

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Blood drops dried on EZ-SPOT® (Charles River), body swabs, fecal pellets, and lung tissue were harvested according to Charles River sampling guidelines and screened for pathogens by PCR and Serology with the Mouse PRIATM (PCR Rodent Infectious Agent) Panel Surveillance Plus and the Serology Profile Assessment Plus by Charles River infectious agent testing (Charles River). A mouse was considered pathogen- exposed if it tested positive in at least one of these assays. Equivocal results were counted as positive. The fraction of positive mice was calculated and visualized using a heatmap created using the pheatmap (Kolde, 2015 ) and RColorBrewer (Neuwirth, 2014 ) packages in Rv3.3.0 (The R Foundation, https://cran.r-project.org/).
Rosshart S.P., Vassallo B.G., Angeletti D., Hutchinson D.S., Morgan A.P., Takeda K., Hickman H.D., McCulloch J.A., Badger J.H., Ajami N.J., Trinchieri G., de Villena F.P., Yewdell J.W, & Rehermann B. (2017). Wild Mouse Gut Microbiota Promotes Host Fitness and Improves Disease Resistance. Cell, 171(5), 1015-1028.e13.
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4

Comprehensive Pathogen Screening Protocol

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Serum was collected at the indicated times for antibody screening using EZ-spot followed by a multiplexed fluorometric immunoassay (Charles River). The screening panel includes: mouse hepatitis virus (MHV), Sendai virus, pneumonia virus of mice, minute virus of mice (MVM), mouse parvovirus type 1(MPV), mouse parvovirus type 2, mouse parvovirus-NS1, murine norovirus (MNV), Theiler’s murine encephalomyelitis virus (TMEV), reovirus, rotavirus EDIM, lymphocytic choriomeningitis virus, ectromelia virus, mouse adenovirus 1 and 2, mouse cytomegalovirus, polyoma virus, Mycoplasma pulmonis, Enchephalitozoon cuniculi, cilia-associated respiratory bacillus, and Clostridium piliforme. Relative serology scores for MHV antigens (recombinant A59-strain nucleocapsid protein, purified A-59 viral lysate, and purified S-strain viral lysate) were calculated by Charles River using median fluorescence index. Active pathogen infection was measured by PCR Rodent Infectious Agent (PRIA) array methods (Charles River) in samples collected from oral swabs or fecal material. This panel screened for MHV, MNV, MPV, MVM, Rodentibacter heylii, and Helicobacter species.
Camell C.D., Yousefzadeh M.J., Zhu Y., Prata L.G., Huggins M.A., Pierson M., Zhang L., O’Kelly R.D., Pirtskhalava T., Xun P., Ejima K., Xue A., Tripathi U., Espindola-Netto J.M., Giorgadze N., Atkinson E.J., Inman C.L., Johnson K.O., Cholensky S.H., Carlson T.W., LeBrasseur N.K., Khosla S., O’Sullivan M.G., Allison D.B., Jameson S.C., Meves A., Li M., Prakash Y.S., Chiarella S.E., Hamilton S.E., Tchkonia T., Niedernhofer L.J., Kirkland J.L, & Robbins P.D. (2021). Senolytics reduce coronavirus-related mortality in old mice. Science (New York, N.y.), 373(6552), eabe4832.
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5

Pathogen Screening in Mouse Samples

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Blood drops dried on EZ-SPOT® (Charles River Laboratories), body swabs, oral swabs, fecal pellets, and lung tissue were harvested according to Charles River Laboratories sampling guidelines and screened for pathogens by PCR and Serology with the Mouse PRIA™ (PCR Rodent Infectious Agent) Panel Surveillance Plus and the Serology Profile Assessment Plus by Charles River infectious agent testing (Charles River Laboratories). A mouse was considered pathogen-exposed if it tested positive in at least one of these assays.
Rosshart S.P., Herz J., Vassallo B.G., Hunter A., Wall M.K., Badger J.H., McCulloch J.A., Anastasakis D.G., Sarshad A.A., Leonardi I., Collins N., Blatter J.A., Han S.J., Tamoutounour S., Potapova S., Foster St. Claire M.B., Yuan W., Sen S.K., Dreier M.S., Hild B., Hafner M., Wang D., Iliev I.D., Belkaid Y., Trinchieri G, & Rehermann B. (2019). Laboratory mice born to wild mice have natural microbiota and model human immune responses. Science (New York, N.Y.), 365(6452), eaaw4361.
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