Affinity of IDO-Gal3 for lactose was determined using affinity chromatography in an AKTA Pure chromatography system (GE Life Sciences) equipped with consumer-packable glass column (GE Life Sciences) packed with α-lactose agarose affinity resin (Sigma-Aldrich). Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich) in phosphate buffer.
α lactose agarose affinity resin
Manufactured by Merck Group
α-lactose agarose affinity resin is a chromatographic media used for the purification of proteins and enzymes. It consists of agarose beads covalently linked to α-lactose, which acts as a ligand for the selective capture of target biomolecules. The resin's core function is to facilitate the separation and enrichment of proteins or enzymes that exhibit specific binding affinities towards the immobilized α-lactose moiety.
Automatically generated - may contain errors
2 protocols using α lactose agarose affinity resin
1
Lactose-Agarose Affinity Chromatography
2
Affinity Chromatography of IDO-Gal3 and Lactose
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