Precision plus protein kaleidoscope prestained protein standard

Manufactured by Bio-Rad

Sourced in United States

Precision Plus Protein Kaleidoscope Prestained Protein Standards is a laboratory product used for protein molecular weight determination. It provides a set of prestained protein standards with defined molecular weights for use in SDS-PAGE analysis.

Automatically generated - may contain errors

Lab products found in correlation

Laemmli sample buffer, by Bio-Rad (3 mentions) Criterion tgx precast gel, by Bio-Rad (2 mentions) Hiload 16 600 superdex 200 pg, by Cytiva (2 mentions) Phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Laemmli buffer, by Bio-Rad (2 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Simplyblue safestain, by Thermo Fisher Scientific (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Hrp conjugated secondary antibody, by GE Healthcare (2 mentions) Amersham ecl prime western blotting detection reagent, by GE Healthcare (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Cy5 nhs, by Lumiprobe (2 mentions) Qubit fluorometer, by Thermo Fisher Scientific (2 mentions) Precast polyacrylamide gel, by Bio-Rad (1 mentions) Goat anti rabbit igg ap conjugate, by Merck Group (1 mentions) Custom oligonucleotides, by Integrated DNA Technologies (1 mentions) Pcr purification kit, by Qiagen (1 mentions) Quick ligation kit, by New England Biolabs (1 mentions) T4 dna ligase buffer, by Thermo Fisher Scientific (1 mentions)

27 protocols using precision plus protein kaleidoscope prestained protein standard

SDS-PAGE Analysis of PEG-Conjugated Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were mixed with Laemmli buffer (Bio-Rad, 1610747) or reduced in 10% 2-mercaptotheanol (BME, Sigma, M6250) in Laemmli buffer at a 3:1 ratio and then run on a 4%–20% precast polyacrylamide gel (Bio-Rad, 4561096). Reduced samples were heated at 95°C for 5 min. 10 μL of each sample was loaded into the gel and run at 160 V for 40 min in 1× Tris-glycine-SDS (TGS) running buffer (Bio-Rad, 1610732). Precision Plus Protein Kaleidoscope prestained protein standard (Bio-Rad, 1610375) was loaded to well number 1 for MW analysis.
The presence of PEG was determined by incubating the gels in a barium iodide stain. 5% barium chloride in 1 M HCl was added to a 0.1 N iodine solution at a 5:2 ratio.³³ (link)
The presence of protein was determined by incubating the gels in Coomassie blue stain (0.05 g of Coomassie brilliant blue R dye [Sigma, B7920], 45 mL of methanol, 10 mL of acetic acid, and 45 mL of water) for 2 h and then destaining overnight in a solution of 50% methanol, 10% acetic acid, and 40% water.

Hill C., Grundy M., Bau L., Wallington S., Balkaran J., Ramos V., Fisher K., Seymour L., Coussios C, & Carlisle R. (2021). Polymer stealthing and mucin-1 retargeting for enhanced pharmacokinetics of an oncolytic vaccinia virus. Molecular Therapy Oncolytics, 21, 47-61.

+ Open protocol

+ Expand

Western Blot Analysis of gB Vaccine

Check if the same lab product or an alternative is used in the 5 most similar protocols

4–12% Bis-Tris gels were loaded with 10 µl of precision plus protein kaleidoscope prestained protein standard from BioRad and 2 ug of protein in NuPAGE LDS 4X sample buffer (ThermoFisherScientific) per well. Proteins were run in duplicate. One gel was stained with Coomassie. The second gel was transferred using an iBlot 2 gel transfer device. The membrane was blocked for 1 h with 1X PBS/1% Casein blocker. Primary antibody (day 140 serum from gB protein vaccinated rabbit) was added to the blocking buffer and incubated overnight at 4°. Next day the membrane was washed 3X with 1XPBS with 0.1% Tween-20. Secondary antibody goat anti-rabbit IgG-AP conjugate (Sigma Cat. No.AP132A) at a 1:1000 dilution was added and incubated 1 h at room temperature. The membrane was washed 3X with 1XPBS with 0.1% Tween-20. Antibody binding was detected using western blue stabilized substrate for alkaline phosphatase. Gel and membrane were imaged with the molecular imager gel doc XR+ system with image lab software. All blots and gels derive from the same experiment and they were processed in parallel.

Valencia S.M., Rochat E., Harnois M.J., Dennis M., Webster H.S., Hora B., Kumar A., Wang H.Y., Li L., Freed D., Zhang N., An Z., Wang D, & Permar S.R. (2023). Vaccination with a replication-defective cytomegalovirus vaccine elicits a glycoprotein B-specific monoclonal antibody repertoire distinct from natural infection. NPJ Vaccines, 8, 154.

+ Open protocol

+ Expand

Engineered Silk-Elastin-Like Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

pET24a(+)
was previously modified for PRe-RDL
(recursive directional ligation by plasmid reconstruction) cloning.^{21 (link)} PRe-RDL vectors containing the genes encoding
E^S_m = (GSGVP)_m (with m = 40, 60, and 80) were developed
previously.^{35 (link)} Custom oligonucleotides for
the construction of the silk blocks S_n^Q = (GAGAGAGQ)_n were synthesized
by Integrated DNA Technologies Inc. Restriction enzymes, calf-intestinal
phosphatase (CIP), and Quick Ligation kit were ordered from New England
Biolabs, and T4 DNA ligase buffer was purchased from Invitrogen. The
DNA miniprep, gel purification, and PCR purification kits were purchased
from Qiagen Inc. Chemically competent Escherichia coli cells (EB5Alpha and BL21 (DE3)) were purchased from EdgeBioSystems,
and the TBdry growth media was purchased from MO BIO Laboratories
Inc. The 4–20% Ready Gel Tris–HCl precast gels and Precision
Plus Protein Kaleidoscope Prestained Protein Standard were purchased
from BioRad.

Willems L., Roberts S., Weitzhandler I., Chilkoti A., Mastrobattista E., van der Oost J, & de Vries R. (2019). Inducible Fibril Formation of Silk–Elastin Diblocks. ACS Omega, 4(5), 9135-9143.

+ Open protocol

+ Expand

Purification and Characterization of IgA Multimers

Check if the same lab product or an alternative is used in the 5 most similar protocols

IgA dimers were purified using a 120mL HiLoad™ 16/600 Superdex™ 200pg (Cytiva, #28-9893-35) column on an ÄKTA Pure FPLC system operating at 4°C. After equilibration of the column with PBS each IgA preparation was loaded via a 10mL superloop at a flow rate of 1.25mL/min and 1.5mL fraction were collected. Isolated peaks consistent with IgA multimers, IgA dimers and IgA monomers were detected at 0.3-0.4, 0.4-0.5 and 0.5-0.6 column volumes respectively (Figure S2H). Fractions spanning all three peaks were collected and evaluated individually by running Criterion™ TGX™ precast gels (Biorad 5671095) under non-reducing conditions with a Precision Plus Protein Kaleidoscope Prestained Protein Standard (Biorad #1610375) (Figure S2I).

Scheid J.F., Barnes C.O., Eraslan B., Hudak A., Keeffe J.R., Cosimi L.A., Brown E.M., Muecksch F., Weisblum Y., Zhang S., Delorey T., Woolley A.E., Ghantous F., Park S.M., Phillips D., Tusi B., Huey-Tubman K.E., Cohen A.A., Gnanapragasam P.N., Rzasa K., Hatziioanno T., Durney M.A., Gu X., Tada T., Landau N.R., West AP J.r., Rozenblatt-Rosen O., Seaman M.S., Baden L.R., Graham D.B., Deguine J., Bieniasz P.D., Regev A., Hung D., Bjorkman P.J, & Xavier R.J. (2021). B cell genomics behind cross-neutralization of SARS-CoV-2 variants and SARS-CoV. Cell, 184(12), 3205-3221.e24.

+ Open protocol

+ Expand

Western Blot Immunodetection of IgG

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 0.5 μg of each sample was loaded on a NuPAGE 4–12% Bis-Tris gel (Thermo Fisher Scientific). Precision Plus Protein Kaleidoscope Prestained Protein Standard (Bio-Rad Laboratories, Hercules, CA) was used as the standard marker. The gel was transferred to a polyvinylidene difluoride membrane using an iBlot 2 Transfer device (Invitrogen). The membrane was blocked with goat histology buffer (1% BSA [Sigma-Aldrich], 2% goat serum [Sigma-Aldrich], 0.3% Triton-X [Sigma-Aldrich], and 0.025% 1g/ml sodium azide [Sigma-Aldrich] in PBS) for 30 min at room temperature. Goat anti-human IgG-Fc fragment Ab (A80-104A; Bethyl Laboratories, Montgomery, TX) diluted in 1:1000 in goat histology buffer was added and incubated for 1 h at room temperature. After washing the blot for 5 min in Dulbecco PBS (DPBS) (HyClone, Logan, UT) three times, donkey anti-goat IgG HRP Ab (Abcam, Cambridge, U.K.) in 1:2000 dilution in goat histology buffer was added and incubated for 1 h at room temperature. After washing the blot three times for 5 min in DPBS, the membrane was developed using the ECL Prime Western blotting System (GE Healthcare) and imaged using the Protein Simple FluorChem System.

McNee A., Smith T.R., Holzer B., Clark B., Bessell E., Guibinga G., Brown H., Schultheis K., Fisher P., Ramos S., Nunez A., Bernard M., Graham S., Martini V., Chrun T., Xiao Y., Kash J.C., Taubenberger J.K., Elliott S., Patel A., Beverley P., Rijal P., Weiner D.B., Townsend A., Broderick K.E, & Tchilian E. (2020). Establishment of a Pig Influenza Challenge Model for Evaluation of Monoclonal Antibody Delivery Platforms. The Journal of Immunology Author Choice, 205(3), 648-660.

+ Open protocol

+ Expand

Protein Extraction and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total soluble protein was harvested from HEK293 cells using RIPA buffer with 1× protease inhibitor cocktails (mini-EDTA free) and phosphatase inhibitor cocktails (ThermoFisher Scientific, USA) with western blotting performed as per Conn et al. [26 (link)]. Anti-FLAG M2 antibody (F1804; Sigma-Aldrich USA) was used at 1:2,500 dilution; with goat anti-mouse HRP conjugated secondary antibody (ThermoFisher Scientific, USA) at 1:10,000 dilution. Chemiluminescent detection was carried out using Super Signal West Pico PLUS (ThermoFisher Scientific, USA) and Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standard (Bio-Rad, USA) was used for size estimation.

Conn V.M., Gabryelska M., Marri S., Stringer B.W., Ormsby R.J., Penn T., Poonnoose S., Kichenadasse G, & Conn S.J. (2020). SRRM4 Expands the Repertoire of Circular RNAs by Regulating Microexon Inclusion. Cells, 9(11), 2488.

+ Open protocol

+ Expand

FHR-5 Protein Detection in Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum proteins from patients and a healthy individual were separated on 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions and blotted onto nitrocellulose membrane. After blotting, the membrane was blocked with 4% non-fat dried milk, 1% BSA in PBS solution. Then, the membrane was incubated with a polyclonal goat anti-FHR-5 (Cat. number: AF3845, R&D Systems, Minneapolis, Minnesota, US) or monoclonal mouse anti-FHR-5 antibody (clone #390513, Cat. number: MAB3845, R&D Systems, Minneapolis, Minnesota, US) followed by horseradish peroxidase (HRP)-conjugated secondary antibodies (rabbit anti-goat and goat anti-mouse, respectively; Southern Biotech, Birmingham, AL, USA). Bound antibodies were detected with Clarity Western ECL substrate (Bio-Rad, Hercules, CA, USA). For estimating the molecular weight of the proteins, a protein molecular weight marker containing a mixture of 10 multicolor recombinant proteins was used (Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standard, Bio-Rad, Hercules, CA, USA).

Garam N., Cserhalmi M., Prohászka Z., Szilágyi Á., Veszeli N., Szabó E., Uzonyi B., Iliás A., Aigner C., Schmidt A., Gaggl M., Sunder-Plassmann G., Bajcsi D., Brunner J., Dumfarth A., Cejka D., Flaschberger S., Flögelova H., Haris Á., Hartmann Á., Heilos A., Mueller T., Rusai K., Arbeiter K., Hofer J., Jakab D., Sinkó M., Szigeti E., Bereczki C., Janko V., Kelen K., Reusz G.S., Szabó A.J., Klenk N., Kóbor K., Kojc N., Knechtelsdorfer M., Laganovic M., Lungu A.C., Meglic A., Rus R., Kersnik Levart T., Macioniene E., Miglinas M., Pawłowska A., Stompór T., Podracka L., Rudnicki M., Mayer G., Rysava R., Reiterova J., Saraga M., Seeman T., Zieg J., Sládková E., Stajic N., Szabó T., Capitanescu A., Stancu S., Tisljar M., Galesic K., Tislér A., Vainumäe I., Windpessl M., Zaoral T., Zlatanova G., Józsi M, & Csuka D. (2021). FHR-5 Serum Levels and CFHR5 Genetic Variations in Patients With Immune Complex-Mediated Membranoproliferative Glomerulonephritis and C3-Glomerulopathy. Frontiers in Immunology, 12, 720183.

+ Open protocol

+ Expand

Analyzing Allergen Protein Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols

The protein composition of each ALK-Abello allergen extract lot and mixes were analysed using equal PNU/mL concentrations and volume (30 μL/lane) by SDS-PAGE. Extracts were diluted to the same PNU concentration and treated with the reducing agent, dithiothreitol (DTT) and loaded in buffer (#1610737 29 Laemmli Sample Buffer, Bio-Rad Laboratories, Inc.; Hercules, CA, USA), then heated to >95°C for 5 min before loading into the wells of pre-cast 10–20% tris-glycine gels (#XP10200BOX, Novex™ WedgeWell, Life Science, Invitrogen; Waltham, MA, USA). Electrophoresis was conducted at 100 V/cm for 1 h to separate the proteins in 19 tris-glycine SDS running buffer [#LC2675, Novex™ Tris-Glycine SDS Running Buffer (109), Invitrogen] and subsequently stained with Coomassie stain (#LC6060, SimplyBlue™ SafeStain, Invitrogen) to visualize linear bands. The size of the separated proteins was identified by molecular weight using a pre-stained protein standard (#161–0375, Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standard, Bio-Rad Laboratories).

Abrams S.B., Brock G.N., Palettas M., Bolner M.L., Moore-Sowers T., Plunkett G.A., Cole L.K., Diaz S.F, & Lorch G. (2018). An evaluation of veterinary allergen extract content and resultant canine intradermal threshold concentrations. Veterinary dermatology, 29(6), 496-e167.

+ Open protocol

+ Expand

Quantitative Western Blot Analysis of Huntingtin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total soluble protein was harvested from HEK293 and SH-SY5Y cells using RIPA buffer with 1× protease inhibitor (mini-EDTA free) and phosphatase inhibitor cocktails (ThermoFisher Scientific, USA). Protein was quantified by Bradford Assay (BioRad, Hercules, CA, USA), and 25 μg of protein was loaded on Any kD™ Mini-PROTEAN^® TGX Stain-Free™ precast PAGE gels (BioRad) and imaged using ChemiDoc imager (BioRad). Total protein loading was quantified and used to normalise between samples using the ChemiDoc software. Membranes were blocked with 5% skim milk powder in tris buffered saline with 0.1% (v/v) Tween-20 (TBS-T) for 1 h at room temperature. Anti-HTT, rabbit monoclonal primary antibody (ab109115; Abcam, Cambridge, UK) was diluted 1:1000 in blocking solution and probed overnight at 4 °C with rocking. Following five washes with TBS-T, goat anti-rabbit HRP-conjugated secondary antibody (ThermoFisher Scientific) at 1:10,000 dilution was used as a secondary antibody. After a further five washes, chemiluminescent detection was carried out using SuperSignal West Pico PLUS reagent (ThermoFisher Scientific). Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standard (Bio-Rad) was used for size estimation.

Gantley L., Stringer B.W., Conn V.M., Ootsuka Y., Holds D., Slee M., Aliakbari K., Kirk K., Ormsby R.J., Webb S.T., Hanson A., Lin H., Selth L.A, & Conn S.J. (2023). Functional Characterisation of the Circular RNA, circHTT(2-6), in Huntington’s Disease. Cells, 12(9), 1337.

+ Open protocol

+ Expand

Purification of IgA multimers, dimers, and monomers

Check if the same lab product or an alternative is used in the 5 most similar protocols

IgA dimers were purified using a 120mL HiLoad^™ 16/600 Superdex^™ 200pg (Cytiva, #28-9893-35) column on an ÄKTA Pure FPLC system operating at 4°C. After equilibration of the column with PBS each IgA preparation was loaded via a 10mL superloop at a flow rate of 1.25mL/min and 1.5mL fraction were collected. Isolated peaks consistent with IgA multimers, IgA dimers and IgA monomers were detected at 0.3–0.4, 0.4–0.5 and 0.5–0.6 column volumes respectively (Figure S2H). Fractions spanning all three peaks were collected and evaluated individually by running Criterion^™ TGX^™ precast gels (Biorad 5671095) under non-reducing conditions with a Precision Plus Protein^™ Kaleidoscope^™ Prestained Protein Standard (Biorad #1610375) (Figure S2I).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!