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Mmp 9 dmp900

Manufactured by R&D Systems
Sourced in Germany

MMP-9 (DMP900) is a laboratory product used for the detection and quantification of Matrix Metalloproteinase-9 (MMP-9), an enzyme involved in various biological processes. This product is designed to assist researchers in their investigations, providing a tool for analyzing MMP-9 levels in samples. The core function of this product is to enable the measurement of MMP-9 concentrations, without making any claims or interpretations about its intended use.

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3 protocols using mmp 9 dmp900

1

ELISA Quantification of Plasma Biomarkers

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The concentrations of plasma or poor‐plate plasma (targeted MMP‐9) were determined using a commercial ELISA Kits. CEACAM‐1 (DY2244) and MMP‐9 (DMP900) were purchased from R&D, while the NGAL (443407) was purchased from Biolegend. The absorbance was determined at 450 nm using an ELISA reader (iMark Microplate Reader, Bio‐Rad). The limitation of detection for CEACAM‐1, MMP‐9, and NGAL was 93.8 pg/ml, 0.156 ng/ml, and 16.4 pg/ml, respectively.
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2

Quantification of Oxidative Stress Markers

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The 3-nitrotyrosine (3NT) ELISA Kit (KA0445) was purchased from ABNOVA c/o EMBLEM (Heidelberg, Germany), MMP-9 (DMP900) and VEGF (DVE00) ELISA Kits were purchased from R&D Systems Europe (Bucharest, Romania). Sulphanilamide (SULF), N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDD), vanadium (III) chloride (VCl3), methanol, diethylether, xylenol orange [o-cresosulfonphthalein-3,3-bis (sodium methyliminodiacetate)], ortho dianisidine dihydrochloride (3-3′-dimethoxybenzidine), ferrous ammonium sulphate, hydrogen peroxide (H2O2), sulphuric acid, hydrochloride acid, glycerol, trichloroacetic acid (TCA), and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Merck (Darmstadt, Germany) and Sigma-Aldrich (Taufkirchen, Germany). Analytical grade chemicals were used exclusively.
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3

Quantifying Plasma Biomarkers via ELISA

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The concentrations of plasma or poor-plate-plasma (targeted MMP-9) were determined using a commercial ELISA Kits. CEACAM-1 (DY2244) and MMP-9 (DMP900) were purchased from R&D, while the NGAL (443407) was purchased from Biolegend. The absorbance was determined at 450 nm using an ELISA reader (iMark Microplate Reader, Bio-Rad). The limitation of detection for CEACAM-1, MMP-9 and NGAL was 93.8 pg/ml, 0.156 ng/ml, and 16.4 pg/ml, respectively.
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