Bone marrow cells were isolated from femoral head after informed consent from patients undergoing hip-replacement surgery. The marrow were mixed with culture medium (MesenPRO RS™ Medium, Gibco, 12746-012) and isolated by h-BM-MSC isolation kit (TBD). The collected cells were plated in tissue culture flasks without further interference for 2–3 days. The culture medium was depleted by successive changes of culture medium (MesenPRO RS™ Medium, Gibco, 12746-012). A confluent monolayer culture with cells was observed 7 days following initial plating. Human BM-MSCs is characterized by the BD human MSC analysis kit (BD 562245).
Mesenpro rs medium
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Switzerland
The MesenPRO RS™ Medium is a serum-free, xeno-free culture medium designed for the growth and expansion of human mesenchymal stem cells (hMSCs) in vitro. The medium is formulated to support the maintenance of the multipotent differentiation potential of hMSCs.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Glutamax, by Thermo Fisher Scientific (6 mentions)
Trypsin edta, by Thermo Fisher Scientific (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Trypsin edta, by Merck Group (4 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Stempro adipogenesis differentiation medium, by Thermo Fisher Scientific (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Human mscs, by Thermo Fisher Scientific (2 mentions)
Mm 1r, by American Type Culture Collection (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Roswell park memorial institute (rpmi), by Lonza (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Oil red o, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Euroclone (2 mentions)
Mesenpro rs growth supplement, by Thermo Fisher Scientific (2 mentions)
Human msc analysis kit, by BD (2 mentions)
Stempro adipogenesis differentiation kit, by Thermo Fisher Scientific (2 mentions)
71 protocols using mesenpro rs medium
1
Isolation and Characterization of Human Bone Marrow-Derived Mesenchymal Stem Cells
2
Isolation and Characterization of Human Bone Marrow Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bone marrow cells were isolated from the femoral head after informed consent from patients undergoing hip-replacement surgery. The marrow were mixed with culture medium (MesenPRO RS™ Medium, Gibco, 12746–012) and isolated by the h-BM-MSC Isolation Kit (TBD). The collected cells were plated in tissue culture flasks without further interference for 2–3 days. The culture medium was depleted by successive changes of culture medium (MesenPRO RS™ Medium, Gibco, 12746–012). A confluent monolayer of cells was observed 7 days following initial plating. Human BM-MSCs were characterised by the BD Human MSC Analysis Kit (BD 562245).
3
Inflammatory Cytokine Modulation of MSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MSCs were treated with IL-1 (Abcam, USA) at a concentration of 10 ng/mL for 24 h. Subsequently, IL-1 receptor antagonist (Abcam, USA) was added to MSCs culture. The culture supernatants were collected for the ELISA assay. For the CM, MSCs (2x104) were cultured in 24-well plates and treated with 10 ng/mL IL-1. Next, MSCs were washed with PBS twice and cultured in fresh MesenPRO RS™ Medium (Thermo Fisher Scientific, USA) as described above. The CM (MSCs-IL-1-CM) was collected 24 h later. The CM collected from MSCs without IL-1 treatment was used as the control (MSCs-CM).
4
Mesenchymal Progenitor Cell Expansion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Freshly detached MPCs were seeded in T75 flasks (20,000 cells/cm2) and let adhere overnight in DMEM/10%PhABS. The day after the medium was changed with MesenPRO® RS medium (Thermo Scientific) and the cells were cultured until confluence (P1-MSCs). Cells were then detached by enzymatic digestion with TrypLE® Select and sub-cultured at 5000 cells/cm in new T75 flasks for flow cytometry analysis, and in 2-well culture chamber slides for the detection of nestin (performed at confluence, P2-MSCs).
5
Cell Culture Conditions for Cancer Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The breast cancer cell line, MDA-MB-231 (ATCC, VA, USA) was cultured in DMEM (Lonza, Switzerland). The multiple myeloma cell line MM1R (ATCC, VA, USA) was cultured in RPMI (Lonza, Switzerland). The colorectal cancer cell line RKO (ATCC, VA, USA) was cultured in McCoys (Lonza, Switzerland). All media were supplemented with 10% foetal bovine serum (FBS) (Thermo Fisher Scientific, MA, USA), 100 U/ml penicillin and 100 μg/ml streptomycin. Human MSCs were from Thermo Fisher Scientific (MA, USA) and cultured in MesenPro RS medium (Thermo Fisher Scientific, MA, USA).
6
Efficient iPSC, MSC, and Cardiomyocyte Generation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transient and stable iPSC lines were generated using the NEON Transfection system (Thermo Fisher Scientific) according to the manufacturer’s recommendation. Briefly, iPSCs were harvested with Accutase, washed twice with PBS, and resuspended at a concentration of 2 × 107 cells/mL in 10 or 100 μL of Buffer R containing the desired DNA. Cells were co-electroporated with the target lux vectors and a transposase-expressing vector. Immediately following electroporation, cells were diluted in prewarmed E8 containing Y27632 and plated. MSC and cardiomyocyte electroporations were performed identically, except that cardiomyocytes were recovered in Advanced RPMI (Thermo Fisher Scientific) and MSCs were recovered in MesenPRO RS medium (Thermo Fisher Scientific).
7
Establishment and Culture of DDLPS Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human DDLPS cell lines (LP6 and LPS12) were previously established and kindly provided by Dr Andrew J. Wagner (Dana Farber Cancer Institute; USA). Human adipose-derived stem cells (ADSCs) were purchased from Invitrogen; Thermo Fisher Scientific, Inc. (cat. R7788115). LP6 cells were cultured in RPMI-1640 medium (cat. 11875093; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; cat. 10270-106; Thermo Fisher Scientific, Inc.). LPS12 cells were cultured in DMEM/F12 (cat. 21331020; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS and GlutaMAX (cat. 35050061; Thermo Fisher Scientific, Inc.). ADSCs were cultured in MesenPRO RS™ medium (cat. 12746012; Thermo Fisher Scientific, Inc.). All cell cultures were treated with 1% antibiotic-antimycotic solution (cat. 15240062; Thermo Fisher Scientific, Inc.) and cultured at 37°C in 5% CO2.
8
Primary Fibroblasts and CHO Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human primary dermal fibroblasts were purchased from ATCC (Manassas, Virginia) and cultured in MesenPRO RS medium (Thermo Fisher Scientific KK).
CHO cells and its glycosylation-defective mutants (Lec1 and Lec8) were purchased from ATCC (Manassas, Virginia) and cultured in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM glutamine, and 25 mM HEPES (pH 7.4).
CHO cells and its glycosylation-defective mutants (Lec1 and Lec8) were purchased from ATCC (Manassas, Virginia) and cultured in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM glutamine, and 25 mM HEPES (pH 7.4).
9
hADSCs Culture in MesenPRO RS Medium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hADSCs line used in this study was purchased from Thermo Fisher Scientific (NYSE:TMO; Waltham, MA) and grown in MesenPRO RS™ medium (Thermo Fisher Scientific, NYSE:TMO) up to 70–80% confluence.
10
Multilineage Differentiation of Human ADSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human ADSC (n = 3) from StemPro Human Adipose-Derived Stem Cell Kit (ThermoFisher, Waltham, MA, USA) were cultured at 104 cells/cm2 in MesenPRO RS™ medium (ThermoFisher) with 2% serum (provided in MesenPRO kit). Cells were subpassaged using TryPLE-Express (ThermoFisher). The multilineage differentiation potential was assessed by induced differentiation to adipocytes using StemPro Adipogenesis kit (ThermoFisher) to chondrocytes using the StemPro Chondrogenesis kit (ThermoFisher). The cells were stained with Oil Red O (Sigma-Aldrich, St. Louis, MI, USA) for adipocytes, toluidine blue (Sigma-Aldrich) for chondrocytes, and alizarin red S (Sigma-Aldrich) for osteocytes [19 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!