Human c reactive protein elisa kit

Manufactured by Abcam

Sourced in United Kingdom, United States

The Human C Reactive Protein ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of C-reactive protein (CRP) levels in human serum or plasma samples. The kit utilizes a highly specific antibody pair and a colorimetric detection method to determine CRP concentration.

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ELISAs (10 citations)

4 protocols using human c reactive protein elisa kit

CRP and IL-6 Quantification in Cellular Samples

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The determination of CRP and IL-6 levels in cell extracts and culture supernatants was performed using human C-Reactive-Protein ELISA Kit (Abcam) and human interleukin-6 ELISA Kit (Abcam) according to the manufacturer’s instructions.
CRP was additionally measured with PETIA (particle-enhanced turbidimetric immunoassay) using Dimension EXL (Siemens Healthineers) at the central clinical laboratory of the University Hospital of Heidelberg.

Schimmack S., Yang Y., Felix K., Herbst M., Li Y., Schenk M., Bergmann F., Hackert T, & Strobel O. (2019). C-reactive protein (CRP) promotes malignant properties in pancreatic neuroendocrine neoplasms. Endocrine Connections, 8(7), 1007-1019.

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APACHE II Scoring in Severe Acute Pancreatitis

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It is well documented that APACHE II scores are associated with the severity of SAP and its complications and mortality.^{15 (link)} The APACHE II scores, measured using a Microsoft® APACHE II graded computer program, were recorded. Venous blood samples were taken after a 12-h fast to measure biochemical parameters. The levels of fasting blood glucose (BG), serum amylase (AMY), albumin (ALB), blood calcium, CRP, tumour necrosis factor (TNF)-α, interleukin (IL)-6, IL-10 and IL-17 were all measured using the following colorimetric and enzyme-linked immunosorbent assay (ELISA) kits from Abcam, Cambridge, UK: Glucose Assay Kit (ab65333), Amylase Assay Kit (ab102523), Human Albumin ELISA Kit (ab179887), Calcium Assay Kit (ab102505), Human C Reactive Protein ELISA Kit (ab99995), Human TNF Alpha ELISA Kit (ab181421), Human IL-6 ELISA Kit (ab178013), Human IL-10 ELISA Kit (ab46034) and Human IL-17 ELISA Kit (ab119535) according to the manufacturer’s instructions. The total white blood cell (WBC) count was measured using an automatic haematology analyser (Sysmex XT-2000i; Sysmex Corporation, Kobe, Japan) according to the manufacturer’s instructions. Observed indices also included the time it took to achieve relief of abdominalgia, time of first defaecation, hospitalization time, complications and the cure rate.

Wang X., Xu J., Li J., Cheng Y., Liu L, & Du Z. (2019). Effect of regional arterial infusion combined with early enteral nutrition on severe acute pancreatitis. The Journal of International Medical Research, 47(12), 6235-6243.

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Quantification of Plasma CRP Levels

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The plasma concentration of CRP was determined using the Human C Reactive Protein ELISA kit (abcam, ab99995). Patient plasma was diluted 1:80,000 in 1× Assay Diluent D.

Cacciatore S., Wium M., Licari C., Ajayi-Smith A., Masieri L., Anderson C., Salukazana A.S., Kaestner L., Carini M., Carbone G.M., Catapano C.V., Loda M., Libermann T.A, & Zerbini L.F. (2021). Inflammatory metabolic profile of South African patients with prostate cancer. Cancer & Metabolism, 9, 29.

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Quantification of Serum Amyloid P and C-Reactive Protein in Mouse and Human Plasma

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For detecting serum amyloid P component (SAP) or C-reacting protein (CRP) in mouse plasma, 0.4 or 2 μL of plasma diluted with 14.6 or 16 μL of PBS and 5 or 6 μL of 4x Laemmli Sample Buffer (Bio-Rad Laboratories, CA, USA) was sonicated for 15 min and boiled for 10 min at 95 °C. This mixture was subjected to Western blotting (see “Immunopreciptation and Western blotting”) using antibodies against SAP (1:1,000, AF2558, Bio-Techne Corporation/R&D Systems, MN, USA) and CRP (1:200, ab65842, Abcam, MA, USA). Concentration of CRP in human plasma was measured by Human C Reactive Protein ELISA Kit (Abcam, MA, USA) according to manufacturer’s protocol. Samples were diluted 1:50,000 and measured in duplicates.

Inoue M., Nakashima R., Enomoto M., Koike Y., Zhao X., Yip K., Huang S.H., Waldron J.N., Ikura M., Liu F.F, & Bratman S.V. (2018). Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis. Nature Communications, 9, 5116.

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