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Lambda xl xenon lamp

Manufactured by Sutter Instruments
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The Lambda XL Xenon lamp is a high-intensity, broadband light source designed for a variety of scientific applications. It generates a continuous spectrum of light from the ultraviolet to the infrared range, making it suitable for various experimental setups that require a stable and versatile light source.

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Lab products found in correlation

Brightline full multiband filter set, by IDEX Corporation (3 mentions) 5 mm liquid light guide, by Sutter Instruments (3 mentions) Perfectfocus system, by Sutter Instruments (3 mentions) Ixon3 897 emccd camera, by Oxford Instruments (2 mentions) Perfect focus system, by Nikon (2 mentions) Te2000, by Nikon (2 mentions) Te2000 microscope, by Nikon (1 mentions) Inverted microscope, by Nikon (1 mentions)

Spelling variants of this product

Lambda XL (18 citations) Lambda XL lamp (9 citations) Xenon lamp (6 citations)

6 protocols using lambda xl xenon lamp

1

Quantification of Autophagosome Formation

Cited 1 time
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Cells were incubated in maintenance medium for each respective cell type supplemented with DMSO vehicle or 250nM Torin1 for four hours. For autophagosome puncta quantification, live cells were imaged at 100X using ONI Nanoimager (www.oni.bio), and images were processed and analyzed using Fiji. For optical pulse labeling, an automated microscopy platform was used as previously described48 (link). Briefly, images were obtained at the indicated time points with a Nikon TE2000 microscope equipped with the PerfectFocus system, a high-numerical aperture 20X objective lens and a 16-bit Andor Clara digital camera with a cooled charge-coupled device. Illumination was provided by a Lambda XL Xenon lamp (Sutter) with a liquid light guide. The ASI 2000 stage was controlled by rotary encoders in all three planes of movement. All components were housed in a custom-designed, climate-controlled environmental chamber (InVivo Scientific) kept at 37° C and 5% CO2. The Semrock BrightLine full-multiband filter set (DAPI, FITC, TRITC, Cy5) was used for fluorophore photoactivation (DAPI), excitation and detection (FITC, TRITC). The illumination, filter wheels, focusing, stage movements and image acquisitions were fully automated and coordinated with a mix of proprietary (ImagePro) and publicly available (μManager) software.
Chua J.P., Bedi K., Paulsen M.T., Ljungman M., Tank E.M., Kim E.S., McBride J.P., Colón-Mercado J.M., Ward M.E., Weisman L.S, & Barmada S.J. (2022). Myotubularin-related phosphatase 5 is a critical determinant of autophagy in neurons. Current biology : CB, 32(12), 2581-2595.e6.
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2

Automated Longitudinal Fluorescence Microscopy

Cited 4 times
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Automated longitudinal fluorescence microscopy began 24h post-transfection for 10d, as previously described (Arrasate et al., 2004 (link); Barmada et al., 2010 (link), 2015 (link); Tsvetkov et al., 2013 (link); Archbold et al., 2018 (link); Malik et al., 2018 (link)). Briefly, images were acquired by an inverted Nikon Ti microscope equipped with a 20× objective lens, a PerfectFocus system, a Lambda XL Xenon lamp (Sutter) with 5 mm liquid light guide (Sutter), and either an Andor iXon3 897 EMCCD camera or Andor Zyla4.2 (+) sCMOS camera. All stage, shutter, and filter wheel movements were carried out by custom code written in publicly available software (μManager, ImageJ) (Weskamp et al., 2019 (link)). For OPL, neurons were pulsed with 500msec per frame of 405nm light prior to image acquisition.
Flores B.N., Li X., Malik A.M., Martinez J., Beg A.A, & Barmada S.J. (2019). An Intramolecular Salt Bridge Linking TDP43 RNA Binding, Protein Stability, and TDP43-Dependent Neurodegeneration. Cell reports, 27(4), 1133-1150.e8.
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3

Automated Microscopy Platform for Neuronal Survival

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Experiments involving neuronal survival analysis and optical pulse imaging utilized an automated microscopy platform as described9.25 (link). Briefly, images were obtained with an inverted microscope (Nikon TE2000) equipped with the PerfectFocus system, a high-numerical aperture 20× objective lens and a 16-bit Andor Clara digital camera with a cooled charge-coupled device. Illumination was provided by a Lambda XL Xenon lamp (Sutter) with a liquid light guide. The ASI 2000 stage was controlled by rotary encoders in all three planes of movement. All components were encased in a custom-designed, climate-controlled environmental chamber (In Vivo Scientific) kept at 37°C and 5% CO2. The Semrock BrightLine full-multiband filter set (DAPI, FITC, TRITC, Cy5) was used for fluorophore photoactivation (DAPI), excitation and detection (FITC, TRITC). The illumination, filter wheels, focusing, stage movements, and image acquisitions were fully automated and coordinated with a mix of proprietary (ImagePro) and publicly available (ImageJ, μManager) software.
Barmada S.J., Serio A., Arjun A., Bilican B., Daub A., Ando D.M., Tsvetkov A., Pleiss M., Li X., Peisach D., Shaw C., Chandran S, & Finkbeiner S. (2014). Autophagy induction enhances TDP43 turnover and survival in neuronal ALS models. Nature chemical biology, 10(8), 677-685.
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4

Longitudinal Fluorescence Microscopy Imaging

Cited 1 time
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Automated longitudinal fluorescence microscopy began 24 h post-transfection for 10d, as previously described [43 (link)–45 ]. Images were acquired using an inverted microscope (Nikon Instruments, NY) with a 20x objective lens, a PerfectFocus system, a Lambda XL Xenon lamp (Sutter Instruments, CA) with 5 mm liquid light guide (Sutter Instruments, CA), and either an Andor iXon3 897 EMCCD camera or Andor Zyla4.2 (+) sCMOS camera. All stage, shutter, and filter wheel movements were done using a custom code written in μManager, ImageJ [45 ].
He F., Flores B.N., Krans A., Frazer M., Natla S., Niraula S., Adefioye O., Barmada S.J, & Todd P.K. (2020). The carboxyl termini of RAN translated GGGGCC nucleotide repeat expansions modulate toxicity in models of ALS/FTD. Acta Neuropathologica Communications, 8, 122.
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5

Automated Longitudinal Fluorescence Microscopy

Cited 4 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Automated longitudinal fluorescence microscopy began 24h post-transfection for 10d, as previously described (Arrasate et al., 2004 (link); Barmada et al., 2010 (link), 2015 (link); Tsvetkov et al., 2013 (link); Archbold et al., 2018 (link); Malik et al., 2018 (link)). Briefly, images were acquired by an inverted Nikon Ti microscope equipped with a 20× objective lens, a PerfectFocus system, a Lambda XL Xenon lamp (Sutter) with 5 mm liquid light guide (Sutter), and either an Andor iXon3 897 EMCCD camera or Andor Zyla4.2 (+) sCMOS camera. All stage, shutter, and filter wheel movements were carried out by custom code written in publicly available software (μManager, ImageJ) (Weskamp et al., 2019 (link)). For OPL, neurons were pulsed with 500msec per frame of 405nm light prior to image acquisition.
Flores B.N., Li X., Malik A.M., Martinez J., Beg A.A, & Barmada S.J. (2019). An Intramolecular Salt Bridge Linking TDP43 RNA Binding, Protein Stability, and TDP43-Dependent Neurodegeneration. Cell reports, 27(4), 1133-1150.e8.
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6

Automated Microscopy Platform for Neuronal Survival

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experiments involving neuronal survival analysis and optical pulse imaging utilized an automated microscopy platform as described9.25 (link). Briefly, images were obtained with an inverted microscope (Nikon TE2000) equipped with the PerfectFocus system, a high-numerical aperture 20× objective lens and a 16-bit Andor Clara digital camera with a cooled charge-coupled device. Illumination was provided by a Lambda XL Xenon lamp (Sutter) with a liquid light guide. The ASI 2000 stage was controlled by rotary encoders in all three planes of movement. All components were encased in a custom-designed, climate-controlled environmental chamber (In Vivo Scientific) kept at 37°C and 5% CO2. The Semrock BrightLine full-multiband filter set (DAPI, FITC, TRITC, Cy5) was used for fluorophore photoactivation (DAPI), excitation and detection (FITC, TRITC). The illumination, filter wheels, focusing, stage movements, and image acquisitions were fully automated and coordinated with a mix of proprietary (ImagePro) and publicly available (ImageJ, μManager) software.
Barmada S.J., Serio A., Arjun A., Bilican B., Daub A., Ando D.M., Tsvetkov A., Pleiss M., Li X., Peisach D., Shaw C., Chandran S, & Finkbeiner S. (2014). Autophagy induction enhances TDP43 turnover and survival in neuronal ALS models. Nature chemical biology, 10(8), 677-685.
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