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Nano2

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NaNO2 is a chemical compound that functions as a laboratory reagent. It is a white crystalline solid that is soluble in water. NaNO2 is commonly used in various chemical and analytical procedures, but a detailed description of its core function is not available without the risk of extrapolation or interpretation.

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Lab products found in correlation

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Close spelling variants of this product

Sodium nitrite (NaNO2), (33 citations) Sodium nitrite (NaNO2) 5%, (2 citations) NaNO2 solution (2 citations) 85% orthophosphoric acid sodium nitrite (NaNO2), (2 citations)

157 protocols using nano2

1

Sodium Nitrite-Induced Hypoxia Model in Rats: Evaluating Mesenchymal Stem Cell Therapy

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The animals were randomly divided into seven groups, with 12 rats in each group.
- Control group 1 (C1): Dorsal subcutaneous injection of distilled water (0.1 mL/100 g body weight) for 21 days, followed by sacrifice by decapitation.
- Control group 2 (C2): Dorsal subcutaneous injection of distilled water (0.1 mL/100 g body weight) for 14 days, followed by no intervention for 4 weeks.
- Hypoxic group (HP group): Subcutaneous injection of sodium nitrite (NaNO2) (35 mg/kg/d; Sigma St. Louis, MO, USA, as a pure white powder (assay ≥ 99%) (Bhanumathy et al., 2010) for 21 days, to induce chemical hypoxia
- N-2wR group: Subcutaneous injection of NaNO2 (35 mg/kg/d) for 15 days, followed by no intervention for 28 days.
- N-3wR group: Subcutaneous injection of NaNO2 (35 mg/kg/d) for 21 days, followed by no intervention for 21 days.
- N-2wSC group: Subcutaneous injection of NaNO2 (35 mg/kg/d) for 15 days, followed by one intravenous injection of MSCs via the tail vein (2 × 106 cells/rat) and no injection for 28 days.
- N-3wSC group: Subcutaneous injection of NaNO2 (35 mg/kg/d) for 15 days, followed by one intravenous injection of MSCs (2 × 106 cells/rat), 7-day NaNO2 injection, and 21-day no intervention.
The total time for the experiment was 3 weeks for the C1 and HP groups, and 6 weeks for the rest of the groups as shown in Figure 1.
Ali E.H., Ahmed-Farid O.A, & Osman A.A. (2017). Bone marrow-derived mesenchymal stem cells ameliorate sodium nitrite-induced hypoxic brain injury in a rat model. Neural Regeneration Research, 12(12), 1990-1999.
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2

Sodium Nitrite Pretreatment and Ischemia-Reperfusion

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Thirty dogs of both sexes were used and divided into four experimental groups. In two groups, either saline (I/R control; n = 4) or sodium nitrite (NaNO2, Merck, Darmstadt, Germany, 0.2 µmol/kg/min) was administered intravenously for 20 min (NaNO2 + I/R, n = 6), and 24 h later these dogs were subjected to a 25 min ischemia followed by a rapid reperfusion. In the other two groups, either saline (SC; n = 10) or sodium nitrite (NaNO2; n = 10) was infused, and 24 h later the experiments were terminated without subjecting the animals to I/R. At the end of the experiments, the hearts were stopped by an excess of anesthetic and tissue samples from the area supplied by the LAD were taken for in vitro analyses. The samples were immediately used for mitochondrial measurements and for cardiomyocyte isolation, respectively.
Demeter-Haludka V., Kovács M., Prorok J., Nagy N., Varró A, & Végh Á. (2019). Examination of the Changes in Calcium Homeostasis in the Delayed Antiarrhythmic Effect of Sodium Nitrite. International Journal of Molecular Sciences, 20(22), 5687.
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3

Calibration of Nitrite Sensors

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To determine whether the nitrite sensor can be calibrated ex-situ, we compared the calibration curves obtained from an ex-situ experiment with the small sensor with synthetic nitrite solutions at 0, 5, 10, 15, 20, 25, 35, 40, 45 and 50 mgN/L (NaNO2, assay = 99%, Merck KGaA, Darmstadt, Germany) and an electric conductivity of 16 mS/cm (temperature corrected, NaCl, assay ≥ 99.5%, Merck KGaA, Darmstadt, Germany) and from the in-situ measurements. The in-situ data were collected in November 2018 with the small sensor. This experiment was not conducted with the large sensor.
Furthermore, the signal trend of a synthetic nitrite solution at a nitrite concentration of 25 mgN/L (NaNO2, assay = 99%, Merck KGaA, Darmstadt, Germany) and an electric conductivity of 16 mS/cm (NaCl, assay ≥ 99.5%, Merck KGaA, Darmstadt, Germany) was observed with the small sensor over 400 min while it was stirred with a magnetic stirrer at 400 rpm (color squid white, IKA®, Staufen, Germany). The pH and the dissolved oxygen concentration were measured and logged (pH 340 and Cond 340i, WTW, Weilheim, Germany) in order to be able to investigate the influence of these parameters.
Britschgi L., Villez K., Schrems P, & Udert K.M. (2020). Electrochemical nitrite sensing for urine nitrification. Water Research X, 9, 100055.
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4

Preparation and Delivery of Fluorinated Emulsions

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Emulsions were prepared using di-morpholino-4-yl-phosphinic acid 12, 12, 13, 13, 14, 14, 15, 15, 16, 16, 17, 17, 18, 18, 19, 19, 19-heptadecafluoro-nonadecyl ester (F8H11DMP) (PharmAgra Labs; Brevard, NC) as the surfactant, and the bulk medium phase was composed of PFOB (Fluoromed, L.P.; Round Rock, TX). The water phase consisted of phosphate-buffered saline (PBS; 0.1 M NaCl, pH 8), and either ambrisentan (Duke Small Molecule Synthesis Facility; Durham, NC) or NaNO2 (Sigma-Aldrich; St. Louis, MO) dissolved at the solubility limit (ambrisentan: 150 mg/ml, NaNO2: 820 mg/ml) in PBS.
The F8H11DMP was solubilized in PFOB at a concentration of 1.5% w/v (mg/mL). Next, the PBS (or PBS solution with the API) was added to the surfactant/PFOB solution in a volume ratio 1:9 v/v which has previously shown the best stability (Butz et al., 2002 (link)). The mixture was then emulsified using a D650 Amalgamator (TPC Advanced Technologies; City of Industry, CA) at a rate of 4400 RPM at 40-second intervals. Intrapulmonary treatments were delivered through an endotracheal tube placed in the rats using a Microsprayer® attached to the FMJ-250 syringe (Penn-Century; Wyndmoor, PA).
Ferguson S.K., Pak D.I., Hopkins J.L., Harral J.W., Redinius K.M., Loomis Z., Stenmark K.R., Borden M.A., Schroeder T, & Irwin D.C. (2019). Pre-clinical assessment of a water-in-fluorocarbon emulsion for the treatment of pulmonary vascular diseases. Drug Delivery, 26(1), 147-157.
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5

Coating Glass Tubes with NaNO2

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The NaNO2 coating solution was made by mixing 0.3 g of NaNO2 (Sigma-Aldrich, product no. 563218) with 3 ml of methanol (Sigma-Aldrich, product no. 34860). The mixture solution was transferred into a glass tube (inner diameter of 6.4 mm, outer diameter of 9.6 mm, and length of 81.3 cm) via a pipette (VWR) and was dried by passing air (flow rate of 100 cm3 min−1) through the tube. After the complete evaporation of methanol, the dried solid NaNO2 was coated on the surface of the glass tube.
Yang D., Liu Q., Wang S., Bozorg M., Liu J., Nair P., Balaguer P., Song D., Krause H., Ouazia B., Abbatt J.P, & Peng H. (2022). Widespread formation of toxic nitrated bisphenols indoors by heterogeneous reactions with HONO. Science Advances, 8(48), eabq7023.
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6

Coating Glass Tubes with NaNO2

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The NaNO2 coating solution was made by mixing 0.3 g of NaNO2 (Sigma-Aldrich, product no. 563218) with 3 ml of methanol (Sigma-Aldrich, product no. 34860). The mixture solution was transferred into a glass tube (inner diameter of 6.4 mm, outer diameter of 9.6 mm, and length of 81.3 cm) via a pipette (VWR) and was dried by passing air (flow rate of 100 cm3 min−1) through the tube. After the complete evaporation of methanol, the dried solid NaNO2 was coated on the surface of the glass tube.
Yang D., Liu Q., Wang S., Bozorg M., Liu J., Nair P., Balaguer P., Song D., Krause H., Ouazia B., Abbatt J.P, & Peng H. (2022). Widespread formation of toxic nitrated bisphenols indoors by heterogeneous reactions with HONO. Science Advances, 8(48), eabq7023.
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7

Standardized Compounds for Antioxidant Assays

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The standard compounds, including cyanidin-3-O-galactoside (90% purity), cyanidin-3-O-glucoside (95% purity), cyanidin (95% purity), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (98% purity), 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (98% purity), 2,9-dimethyl-1,10-phenanthroline (Neocuproine) (99% purity), potassium persulfate (99% purity), acetonitrile, formic acid, ethanol, methanol, and dimethyl sulfoxide (DMSO), were obtained from Sigma-Aldrich (Darmstadt, Germany). cyanidin-3-O-galactoside and cyanidin-3-O-arabinoside standards were bought from Polyphenols (Sandnes, Norway). HCl, NaNO2, H2O2, and CuCl2 were purchased from Merck (Darmstadt, Germany). Fetal bovine serum (FBS), Lonza, Dulbecco's Modified Eagle Medium (DMEM), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Lonza Group Ltd. (Basel, Switzerland). Glutamine, penicillin and streptomycin, and amphotericin were purchased from Sigma Chemical Co. (St. Louis, MO). The water used for experiments was treated in a Milli-Q water purification system.
Rugină D., Diaconeasa Z., Coman C., Bunea A., Socaciu C, & Pintea A. (2015). Chokeberry Anthocyanin Extract as Pancreatic β-Cell Protectors in Two Models of Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2015, 429075.
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8

Thermoplastic PU and PEG Hydrogel Synthesis

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Polymers including thermoplastic PU and PEG (2000 MW) were obtained from Desmopan (Leverkusen, Germany) and Sigma-Aldrich (Shanghai, China) respectively. However, gelatin was purchased from Sigma-Aldrich (St. Louis, MO, USA). The solvents of tetrahydrofuran (THF), ethanol, glutaraldehyde, dimethyl sulfoxide (DMSO), hexane, hydrochloric acid (HCL) and the cross-linkers of 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) were bought from Merck (Hohenbrunn, Germany). The other chemical materials such as sulfanilamide, naphtylethelenediamine-dihydrochloride, NaNO2 and H3PO4 were also purchased from Merck too. Aspirin, toluidine blue, CaCl2 and 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) were provided from Sigma-Aldrich (St. Louis, MO, USA). Heparin (Heparin sodium injection parenteral, 5000 U/mL) was provided by DarouPhakhsh (Tehran, Iran). The cell culture medium Dulbecco’s modified Eagle’s medium (DMEM) with high glucose content, fetal bovine serum (FBS), trypsin and phosphate buffered saline (PBS) were purchased from Gibco (Grand Island, NY, USA). Moreover, the HUVEC line was purchased from the Pasture Institute cell bank (Tehran, Iran).
Hosseinzadeh S., Shams F., Fattahi R., Nuoroozi G., rostami E., Shahghasempour L., Salehi-Nik N., Bohlouli M., Khojasteh A., Ghasemi N, & Peiravi H. (2022). Surface Coating of Polyurethane Films with Gelatin, Aspirin and Heparin to Increase the Hemocompatibility of Artificial Vascular Grafts. Advanced Pharmaceutical Bulletin, 13(1), 123-133.
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9

Characterization of K10 Mt Mineral

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The K10 Mt was purchased from Aldrich and used as received. This material has a cation exchange capacity of 0.59 ± 0.01 mmol g−1 and a BET specific surface area of 228 m2 g−1 (Abate and Masini, 2005b (link)). Analytical grade FeCl3, NaOH, KH2PO4, K2HPO4, sodium salt of humic acid, NaNO3, NaNO2, and NH4Cl were from the Merck Group. Fulvic acid was isolated from vermicompost as described elsewhere (Abate et al., 2006 ).
Leite S.T., do Nascimento F.H, & Masini J.C. (2020). Fe(III)-polyhydroxy cations supported onto K10 montmorillonite for removal of phosphate from waters. Heliyon, 6(4), e03868.
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10

Synthesis and Characterization of Novel Aromatic Polyimides

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In this study, the following materials were used: 4,4′-isopropylidene-diphenoxy-bis(phthalic anhydride) (6HDA), which was purchased from Sigma-Aldrich, St. Louis, MO, USA; and 2,6-bis(3-aminophenoxy)benzonitrile (m-CN) and 4-[(4-cyanophenyl)diazenyl]phenol (AzoCN) synthesized in our laboratory using previously reported methods [33 ,34 (link),35 (link),36 ] at M.p. 136–138 and 200–202 °C, respectively. N,N-dimethylacetamide (HPLC grade), p-aminobenzonitrile, phenol (99%) and NaNO2 (99.9%) were purchased from Merck KGaA, Darmstadt, Germany. All reagents and solvents were used as received.
Barzic A.I., Sava I., Albu R.M., Ursu C., Lisa G, & Stoica I. (2023). Polyimide-Derived Supramolecular Systems Containing Various Amounts of Azochromophore for Optical Storage Uses. Polymers, 15(4), 1056.
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