Human iPSC/iPSC-derived NPCs were provided by IxCell Biotechnology Ltd. The human iPSCs-derived NPCs cells were maintained as an adherent culture in 50% DMEM-F12 and 50% Neurobasal Medium, containing N2 supplement, B27 supplement (Minus Vitamin A), NEAA, 1 × Glutamax, 10 ng/mL FGF-Basic Recombinant Human Protein (bFGF, Gibco, New York, NY, USA), 10 ng/mL LIF Recombinant Human Protein (hlif, Gibco, New York, NY, USA), 5 μM SB431542 (Selleckchem, Shanghai, China), 3 μM CHIR99021 (Selleckchem, Shanghai, China), and 200 μM L-Ascorbic acid 2-phosphatesesquimagnesium salt hydrate (Sigma, St. Louis, MO, USA). The cells were maintained at 37 °C in humidified air with 5% CO2.
Fgf basic recombinant human protein
Manufactured by Thermo Fisher Scientific
Sourced in United States
FGF-Basic Recombinant Human Protein is a laboratory reagent produced by Thermo Fisher Scientific. It is a purified protein derived from a recombinant source. The core function of this product is to serve as a tool for research applications.
Automatically generated - may contain errors
Lab products found in correlation
Chir99021, by Selleck Chemicals (1 mentions)
L ascorbic acid 2 phosphate sesquimagnesium salt hydrate, by Merck Group (1 mentions)
Sb431542, by Selleck Chemicals (1 mentions)
Poly l lysine hydrobromide, by Merck Group (1 mentions)
Bmp2 recombinant human protein, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Chloroauric acid trihydrate, by Sinopharm (1 mentions)
2 protocols using fgf basic recombinant human protein
1
Maintenance of Human iPSC-Derived NPCs
2
Biomaterial Fabrication and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Polyethyleneimine
solution (PEI, ∼50%
in H2O), poly-l -lysine hydrobromide (PLL, MW:
30 000–70 000), Hep ammonium salt from porcine
intestinal mucosa (17–19 kDa), and bovine serum albumin were
all purchased from Sigma-Aldrich (USA). FGF Basic Recombinant Human
Protein and BMP-2 Recombinant Human Protein were obtained from Gibco.
Chloroauric acid trihydrate (HAuCl4·4H2O, 99.9%) was purchased from Shanghai Sinopharm (China). Trisodium
citrate dihydrate (Na3C6H5O7·2H2O) was obtained from Tianjin Kaitong chemical
reagent co., Ltd (China). APTT kits were purchased from MACCURA, China.
PLA sheets with a thickness of 100 μm were purchased from the
Institute of Medical Devices of Shandong Province (China). All of
the chemical reagents were used as received without further purification.
The water used in this work was purified using a Millipore Milli-Q
system.
solution (PEI, ∼50%
in H2O), poly-
30 000–70 000), Hep ammonium salt from porcine
intestinal mucosa (17–19 kDa), and bovine serum albumin were
all purchased from Sigma-Aldrich (USA). FGF Basic Recombinant Human
Protein and BMP-2 Recombinant Human Protein were obtained from Gibco.
Chloroauric acid trihydrate (HAuCl4·4H2O, 99.9%) was purchased from Shanghai Sinopharm (China). Trisodium
citrate dihydrate (Na3C6H5O7·2H2O) was obtained from Tianjin Kaitong chemical
reagent co., Ltd (China). APTT kits were purchased from MACCURA, China.
PLA sheets with a thickness of 100 μm were purchased from the
Institute of Medical Devices of Shandong Province (China). All of
the chemical reagents were used as received without further purification.
The water used in this work was purified using a Millipore Milli-Q
system.
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