Cell viability was detected by CCK8 assay (C6030, New Cell & Molecular Biotech Co., Ltd). Briefly, cells were seeded in 96-well plates with a density of 2 × 103 cells/well. After treatments, CCK8 was added to the cell culture medium and maintained for additional 1 h. The absorbance was determined at 450 nm by a microplate reader (BioTek). Nutlin-3a (HY-10029), Siremadlin (HY-18658), Idasanutlin (HY-15676), and HY-100692 were purchased from MCE MedChemExpress. MI773 (S7649) was purchased from Selleck.
Mi773
Manufactured by Selleck Chemicals
Sourced in Germany
MI773 is a laboratory instrument used for analytical and research purposes. It is designed to perform specific functions related to chemical analysis and data collection. The core function of MI773 is to provide accurate and reliable measurements for various applications. No further details or interpretations are provided.
Automatically generated - may contain errors
Lab products found in correlation
Nutlin 3a, by MedChemExpress (1 mentions)
Siremadlin, by MedChemExpress (1 mentions)
Idasanutlin, by MedChemExpress (1 mentions)
Pyrazofurin, by Merck Group (1 mentions)
L bso, by Merck Group (1 mentions)
Doxycycline hyclate, by Merck Group (1 mentions)
Ferrostatin 1, by Merck Group (1 mentions)
Nutlin 3a, by Selleck Chemicals (1 mentions)
Mafosfamide, by Santa Cruz Biotechnology (1 mentions)
Penicillin, by Immunotools (1 mentions)
Streptomycin, by Immunotools (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Rg7388, by MedChemExpress (1 mentions)
Rg7112, by MedChemExpress (1 mentions)
Nutlin 3a, by Merck Group (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
4 protocols using mi773
1
Cell Viability Assay with CCK8
2
Small Molecule Compound Preparation and Storage
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Erastin2 and ML162 were synthesized at Acme Bioscience. Nutlin-3 (Cat. no. S1061), nutlin-3b (Cat. no. S8065), gemcitabine HCL (Cat. no. S1149), and MI-773 (Cat. no. S7649) were purchased from Selleck Chemicals. Pyrazofurin (Cat. no. SML1502), HU (Cat. no. H8627), MPA (Cat. no. M5255), ferrostatin-1 (Cat. no. SML0583), Doxycycline hyclate (Cat. no. D9891), and L-BSO (Cat. no. B2515) were purchased from Sigma-Aldrich. BSO and gemcitabine HCL were dissolved directly into cell media. C11-BODIPY 581/591 was prepared as a stock solution in methanol and stored before use at −20°C. All other compounds were prepared as stock solutions in DMSO and stored before use at −20°C.
3
Dissolution and Dilution of MI-773 and Nutlin-3a
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MI-773 (SAR405838, MI-77301) (Catalogue No. S7649) and Nutlin-3a (Catalogue No. S8059) were purchased from Selleckchem (Munich, Germany). The drugs were dissolved in DMSO to obtain a stock solution, which was kept at 4 °C, and immediately before use further diluted with culture medium to concentrations required for the in vitro experiments.
4
Cultivation of Lung Cancer and MEF Cells
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Human lung cancer cell lines (H1299, H460) were obtained from the American Tissue Collection Center (ATCC) and authenticated by short tandem repeat analysis at the Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. Cells were maintained in high‐glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Sigma‐Aldrich), 100 U/ml penicillin, 100 μg/ml streptomycin (Life Technologies), and 1 μg/ml amphotericin B (Sigma) at 37°C with 5% CO2. Primary MEFs were isolated from E12.5‐13.5 mouse embryos and amplified under low oxygen conditions (3% O2); their gender is not available. Eμ‐Myc lymphoma cell lines were maintained in B‐cell medium: 1:1 mixture of DMEM and Iscove's modified Dulbecco's medium (IMDM) supplemented with 20% FBS, 100 U/ml penicillin/streptomycin, 50 μM 2‐mercaptoethanol, and 1 ng/ml mIL‐7 (ImmunoTools). Cells were cultured on a feeder layer of 30 Gy‐irradiated NIH 3T3 cells. Nutlin‐3a (Sigma) was used at 10 μM, RG7112 (MedChemExpress) at 5 μM, RG7388 (MedChemExpress) at 8 μM, MI773 (Selleckchem) at 10 μM, and Mafosfamide (Santa Cruz) at 1–5 μg/ml as indicated. Hydrogen peroxide (Sigma) was used at 50–800 μM.
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