Atomlab 500 dose calibrator and wipe tester

⁶⁴Cu was purchased from PET radioisotopes manufacturer (Voxel, Cracow, Poland) and was produced in ⁶⁴Ni(p,n)⁶⁴Cu reaction conducted on ALCEO solid target system (Comecer, Italy) coupled with GE PETrace 840 cyclotron (GE Healthcare Technologies Inc., Chicago, IL, USA). The requested activity was about 200 MBq in the form of dried ⁶⁴CuCl₂ reconstituted on-site with 1 mL of water. Stock porphyrin solution, buffers, and 0.05 mol L⁻¹ ascorbic acid (Merck KGaA, Darmstadt, Germany) were prepared as described above. Water and phosphate-buffered saline (PBS) were of pharmaceutical grade. ⁶⁴CuTCPP was synthesized at room temperature by mixing 200 μL of ⁶⁴CuCl₂ solution, 400 μL of TCPP, and 200 μL AA stock solutions, filled up with a borate buffer up to 5 mL, and vortexed. For final purification and formulation solution was loaded on anion exchange Waters Sep-Pak Accell Plus QMA column (Waters Corporation, Milford, CT, USA) with an ISM833 Ismatec peristaltic pump (VWR, Radnor, PA, USA) at 1 mL min⁻¹, flushed with 5 mL of water at 2 mL min⁻¹, acidified with 1 mL of 0.1 mol L⁻¹ HCl and finally eluted with 0.5 mL of ethanol. After evaporation near dryness, small aliquot of PBS was added to reach expected activity concentration. An Atomlab 500 dose calibrator and wipe tester (Biodex, Shirley, NY, USA) was used for activity determination.

An ISMATEC peristaltic pump and 11 Elite (Harvard Apparatus, Holliston, MA, USA) syringe pump were used for liquid transfers, an Atomlab 500 dose calibrator and wipe tester (Biodex, Shirley, NY, USA) was used for activity determination, a Bioscan TLC reader Miniscan (Bioscan, Washington, DC, USA) with BioChrome software was used for thin layer chromatogram evaluations, a Thermo Scientific iCAP 6000ICP OES spectrometer was used for impurity determination. The instrumental settings of the manufacturers were as follows: RF generator power 1.15 kW, auxiliary gas flow 0.5 L min⁻¹, nebulizer gas flow 0.40 L min⁻¹, coolant gas flow 12 L min⁻¹. For stability assessment the Shimadzu AD20 HPLC system with UV-Vis (ultraviolet-visible) and radiometric detector GabiStar (Raytest, Straubenhardt, Germany) was used.