Omnic spectra software

Manufactured by Thermo Fisher Scientific

Sourced in United States

OMNIC Spectra software is a data analysis program designed for use with Thermo Scientific™ Fourier Transform Infrared (FTIR) spectrometers. The software provides core functions for viewing, processing, and analyzing infrared spectral data.

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20 protocols using omnic spectra software

FTIR Analysis of Anthocyanin Complexes

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Pellets (insoluble complexes) obtained after centrifugation of the mixtures were freeze-dried and placed on an iD7 ATR (Thermo Fisher Scientific Inc., Waltham, MA, USA) diamond stage that was attached to a Nicolet™ iS™ 5 FTIR Spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Since no sedimentation was observed in the BCP and WP controls at both pH values, dried powders of the controls were used for the scanning. In addition, the dried form of cyanidin 3-O-rutinoside chloride and delphinidin 3-O-rutinoside chloride were also scanned for comparison with BCP. The FTIR scanning was done at 20 °C and replicated twice for each sample (n = 2)—controls and freeze-dried insoluble fractions—because the recorded spectra between the duplicated scans and samples were highly reproducible. The infrared spectra were recorded within the range of 400–4000 cm⁻¹ and band positions were identified by the Omnic™ Spectra software (Thermo Fisher Scientific Inc., Waltham, MA, USA). The results were averaged, normalised, and plotted using SigmaPlot 14.5 (Systat Software, Inc., Chicago, IL, USA).

Salleh N., Goh K.K., Waterland M.R., Huffman L.M., Weeks M, & Matia-Merino L. (2022). Complexation of Anthocyanin-Bound Blackcurrant Pectin and Whey Protein: Effect of pH and Heat Treatment. Molecules, 27(13), 4202.

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Fourier-Transform Infrared Analysis of RBA Conversion

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DC of the different RBAs
was evaluated using Fourier-Transform Infrared Spectroscopy (FTIR,
Nicolet Nexus 670, Thermo Fisher Scientific, Waltham, MA, U.S.A.)
using a horizontal transmission accessory. Specimens were prepared
by placing one drop of the given RBA between two sodium chloride salt
plates followed by photopolymerization (radiant exposure of 18.3 J/cm²) to create 15 μm-thick films (n = 5 per group). The
spectra were collected from 4000 to 750 cm^–1 at
a resolution of 4 cm^–1 and further analyzed using
the OMNIC Spectra Software (Thermo Fisher Scientific). The conversion
was determined from the ratio of areas under aliphatic C=C
stretching vibration peak (1638 cm^–1) and aromatic
stretching vibration peak (1608 cm^–1) (at full width
at half-maximum) of cured and uncured RBAs and expressed as a percentage.

Alkhazaleh A., Elfagih S., Chakka L.R., Armstrong S.R., Comnick C.L., Qian F., Salem A.K., Guymon C.A., Haes A.J, & Vidal C.M. (2022). Development of Proanthocyanidin-Loaded Mesoporous Silica Nanoparticles for Improving Dental Adhesion. Molecular Pharmaceutics, 19(12), 4675-4684.

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FTIR Analysis of Composite and Epoxy Structures

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Fourier transform infrared spectroscopy (FTIR) was performed to identify and determine functional groups of composite and epoxy structures in the infrared (IR) region between 400 cm

^{- 1}

and 4000 cm

^{- 1}

. The absorption spectra were obtained with a resolution of 4 cm

^{- 1}

and 64 scans in each assay. The equipment used was a Thermo Scientific spectrometer, using the OMNIC Spectra software.

Oliveira M.S., da Luz F.S., Pereira A.C., Costa U.O., Bezerra W.B., da Cunha J.D., Lopera H.A, & Monteiro S.N. (2022). Water Immersion Aging of Epoxy Resin and Fique Fabric Composites: Dynamic–Mechanical and Morphological Analysis. Polymers, 14(17), 3650.

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FTIR Characterization of Ink Mixtures

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Infrared spectra were obtained using a Nicolet iS50 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a KBr/Ge beam splitter, deuterated triglycine sulfate detector, and smart iTX-iD7 attenuated total reflectance sampling accessory with a diamond crystal. The FTIR spectra were collected from 400 cm⁻¹–4000 cm⁻¹. Each ink mixture was scanned 16 times at a resolution of 8 cm⁻¹. The spectra were normalized and corrected for baseline effects using OMNIC spectra software (version 8.0; Thermo Fisher Scientific).

Fan M., Choi Y.J., Wedamulla N.E., Kim S.H., Bae S.M., Yang D., Kang H., Tang Y., Moon S.H, & Kim E.K. (2024). Different particle sizes of Momordica charantia leaf powder modify the rheological and textural properties of corn starch-based 3D food printing ink. Heliyon, 10(4), e24915.

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FTIR Characterization of GG Microspheres

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FTIR was utilized to gauge the appropriate formation of GG microspheres and to ensure divalent ion crosslinking. For this, samples were lyophilized, and spectra were acquired using an FTIR spectrophotometer (Nicolet iS10; ThermoFischer Scientific, Waltham, MA, USA) for both GG powder and GG microspheres. The equipment was managed in the OMNIC Spectra software (ThermoFischer Scientific), and spectra were collected operating in ATR mode with an average of 120 scans on wavenumbers ranging from 400–4000 cm⁻¹, at a resolution of 32 cm⁻¹.

Batista-Silva J., Gomes D., Barroca-Ferreira J., Gallardo E., Sousa Â, & Passarinha L.A. (2023). Specific Six-Transmembrane Epithelial Antigen of the Prostate 1 Capture with Gellan Gum Microspheres: Design, Optimization and Integration. International Journal of Molecular Sciences, 24(3), 1949.

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FTIR Characterization of Polymer Samples

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ATR-FTIR-spectra were recorded using a Nicolet iS5 FTIR-spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a DTGS detector (Thermo Fisher Scientific, Waltham, MA, USA). IPECs, raw polymers (i.e., C71G, C2020, C10, NAA-1) and vacuum-dried IPEC-CP were directly placed on the iD5 smart single bounce ZnSe ATR crystal. The spectra were analysed using OMNIC spectra software (Thermo Fisher Scientific, Waltham, MA, USA).

Garipova V.R., Gennari C.G., Selmin F., Cilurzo F, & Moustafine R.I. (2018). Mucoadhesive Interpolyelectrolyte Complexes for the Buccal Delivery of Clobetasol. Polymers, 10(1), 85.

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Structural Analysis of Dentin Collagen

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The structural and biochemical variation of the dentin collagen before and after protein destabilization was assessed using ATR-FTIR spectroscopy (Nicolet 6700 and Smart iTR, Thermo Fisher Scientific, Waltham, MA, USA). Dentin specimens (2 × 6 × 0.5 mm, width × length × thickness, n = 3) were demineralized and treated as described in sections 2.1 and 2.2. Absorbance spectra were collected in the range of 650 to 4000 cm⁻¹, using 128 acquired scans and a resolution of 4.0 cm⁻¹. The peak area of specific bands were determined following a two-point baseline correction, normalization, and band area integration using OMNIC Spectra Software (Thermo Fisher Scientific, USA). Variation in the secondary structure of the collagen triple helix was calculated by the ratio of the peak area of amide III (1240 cm⁻¹) to the CH₂ scissoring (1450 cm⁻¹) bands [22 (link),23 (link)].

Alania Y., Cavalcante dos Reis M., Nam J.W., Phansalkar R.S., McAlpine J., Chen S.N., Pauli G.F, & Bedran-Russo A.K. (2020). A dynamic mechanical method to assess bulk viscoelastic behavior of the dentin extracellular matrix. Dental materials : official publication of the Academy of Dental Materials, 36(12), 1536-1543.

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FTIR-ATR Analysis of PVA Substrates

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FTIR-ATR (Nicolet iS5, Thermo Fisher Scientific, United States) was used to confirm the absorption bands of amide I (1,600–1,700 cm⁻¹) and amide II (1,500–1,600 cm⁻¹) groups, the most prominent absorption bands of proteins, of the prepared PVA substrates. Briefly, samples are dried in a desiccator at R.T. and were sandwiched between the reflection crystal (KRS-5) and the back plate. Spectra were taken with air as the background using OMNIC spectra software (OMNIC 9.12.1019, Thermo Fisher Scientific, United States) in the range of 400–4,000 cm⁻¹ (Schwaighofer and Lendl, 2023 (link)).

Wu I.C., Liou J.W., Yang C.H., Chen J.H., Chen K.Y, & Hung C.H. (2023). Self-assembly of gelatin and collagen in the polyvinyl alcohol substrate and its influence on cell adhesion, proliferation, shape, spreading and differentiation. Frontiers in Bioengineering and Biotechnology, 11, 1193849.

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Infrared Spectroscopy of Cactus Fruit Extracts

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The infrared spectroscopy analyses of Epiphyllum, Hylocereus, and Opuntia fruits’ dry methanolic extracts were performed on a Nicolet FT-IR 6700 spectrometer (Thermo Electron Corporation, Cambridge, UK) with Omnic™ Spectra Software (Thermo Scientific, Waltham, MA, USA), in attenuated total reflectance sampling (ATR) mode. The infrared spectrum was measured in the mid-infrared region (4000–600 cm⁻¹). The position and intensity of the absorption bands in the FTIR spectra were used to analyse the functional groups according to libraries and bibliography [30 (link),31 ,32 ,33 (link)].

Barkociová M., Tóth J., Sutor K., Drobnicka N., Wybraniec S., Dudík B., Bilková A, & Czigle S. (2021). Betalains in Edible Fruits of Three Cactaceae Taxa—Epiphyllum, Hylocereus, and Opuntia—Their LC-MS/MS and FTIR Identification and Biological Activities Evaluation. Plants, 10(12), 2669.

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Photocurable Formulations Characterization

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The FT-IR spectra were recorded with a Nicolet iS 50 Spectrometer with 16 scans from 4000 to 500 cm⁻¹ in transmission mode in air through a resolution of 4 cm⁻¹. The photocurable formulations were coated on a silicon wafer, with a thickness of 25 μm, and subsequently analysed before and after the irradiation under the fusion lamp. The Thermo Scientific™ OMNIC™ Spectra Software was used to record and process the data.

Malburet S., Di Mauro C., Noè C., Mija A., Sangermano M, & Graillot A. (2020). Sustainable access to fully biobased epoxidized vegetable oil thermoset materials prepared by thermal or UV-cationic processes. RSC Advances, 10(68), 41954-41966.

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