Log In Sign up
The largest database of trusted experimental protocols

Axio observer light microscope

Manufactured by Zeiss
Visit Supplier
Sourced in Germany

The Axio Observer Light Microscope is a high-performance optical microscope designed for a wide range of applications. It features a stable, ergonomic design and advanced optical components to deliver precise, high-quality imaging. The microscope is equipped with a range of illumination options and can accommodate a variety of specimen types and observation techniques, making it a versatile tool for various research and analysis tasks.

Automatically generated - may contain errors

Lab products found in correlation

Apotome 2, by Zeiss (2 mentions) Spinning disc confocal microscope, by PerkinElmer (1 mentions) Volocity, by PerkinElmer (1 mentions) Ab16669, by Abcam (1 mentions) Ab4059, by Abcam (1 mentions) Ab52488, by Abcam (1 mentions) Ab93684, by Abcam (1 mentions) Ab183685, by Abcam (1 mentions) Ab91279, by Abcam (1 mentions) Ab150077, by Abcam (1 mentions) Human insulin, by Merck Group (1 mentions) Fibronectin, by Roche (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Hematoxylin and eosin staining, by Merck Group (1 mentions) Neutral buffered formalin, by CellPath (1 mentions) Anti mpo, by Abcam (1 mentions) Anti cd206, by Abcam (1 mentions) Anti ly6g, by Abcam (1 mentions) Pt link system, by Agilent Technologies (1 mentions) Anti foxp3, by Cell Signaling Technology (1 mentions)

9 protocols using axio observer light microscope

1

Organoid Assays for Malat1 ASO Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
Organoids from MMTV-PyMT and MMTV-Cre;Flox neo-neu NT tumors were prepared and cultured as described previously (Ewald 2013 (link)). Organoids were mixed with Matrigel and plated in 24-well Mat-Tek dishes. For ASO treatment, 500 nM Malat1 ASO or scASO was added to 1 mL of organoid culture medium. Images were acquired using a Zeiss Axio-Observer light microscope. For live-cell imaging, organoids from MMTV-PyMT or MMTV-PyMT;CAG::H2B-EGFP mice were treated with Malat1 or ScASOs. At least five organoids per well were imaged in three dimensions over a period of 4 d at 15- or 30-min intervals using a Perkin-Elmer spinning-disc confocal microscope. Time-lapse images were processed using Volocity (PerkinElmer) and ImageJ (National Institutes of Health) software.
Arun G., Diermeier S., Akerman M., Chang K.C., Wilkinson J.E., Hearn S., Kim Y., MacLeod A.R., Krainer A.R., Norton L., Brogi E., Egeblad M, & Spector D.L. (2016). Differentiation of mammary tumors and reduction in metastasis upon Malat1 lncRNA loss. Genes & Development, 30(1), 34-51.
+ Open protocol
+ Expand
2

Immunostaining of Murine Melanoma Tumors

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Deparaffinization and antigen retrieval were performed in murine melanoma subcutaneous tumors using Antigen Retrieval Buffer (Abcam ab52488 and ab93684) and stained as previously described.20 (link) The following antibodies were used for immune staining as described previously:5 (link) anti-CD3 (ab16669), anti-CD4 (ab183685), anti-CD8 (ab22378), anti-granzyme B (ab4059), anti-F4/80 (ab6640), anti-iNOS (ab3523), anti-Arg.1 (ab91279), anti-LDHA (ab52488), and Goat Anti-Rabbit Alexa 488 (ab150077), all antibodies were purchased from Abcam. Images were obtained by using an Axio Observer Light Microscope with the Apotome.2 (Zeiss). Metastatic melanoma lesions were gated by generating a region of interest, and threshold merged fluorescence limited to ROI and calculated using the ImageJ software.
de Azevedo R.A., Shoshan E., Whang S., Markel G., Jaiswal A.R., Liu A., Curran M.A., Travassos L.R, & Bar-Eli M. (2020). MIF inhibition as a strategy for overcoming resistance to immune checkpoint blockade therapy in melanoma. Oncoimmunology, 9(1), 1846915.
+ Open protocol
+ Expand
3

Insulin-induced FoxO1 Translocation in Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
3T3-L1 fibroblasts were differentiated on fibronectin (Roche Applied Science, Almere, the Netherlands) coated 96 well plates and differentiated. Mature adipocytes were transduced with Lentivirus particles containing FoxO1-Clover. FoxO1 is a direct and specific target of Akt; FoxO1 rapidly translocate from the nucleus to the cytoplasm in response to Akt activation (29 (link)). The transduction was followed by a 24 hour incubation with 0 or 1 mmol/L Mg2+ of which the last 6 hours adipocytes where incubated without FBS. Medium was replaced 30 minutes prior to starting live imaging; containing 0 or 1 mmol/L Mg2+, FBS free, phenol-free, supplemented with 1 μg/ml Hoechst 33342 (Sigma-Aldrich). Live imaging was performed using a ZEISS Axio Observer light microscope. Adipocytes were imaged for 30 minutes with a photo interval per 30 seconds. The cells were stimulated using a final concentration of 10 nM human insulin (Sigma-Aldrich) which was added between 30-60 seconds after the start of visualization. The relative intensity of fluorescent units in the cytosol/nucleus was analyzed by the following formula:
All values are normalized by dividing by the first measurement (t=0). Data was fitted using the four-parameter sigmoid dose-response curve. The maximum top value of this function was used as the fluorescent unit’s cytosol/nucleus ratio.
Oost L.J., Kurstjens S., Ma C., Hoenderop J.G., Tack C.J, & de Baaij J.H. (2022). Magnesium increases insulin-dependent glucose uptake in adipocytes. Frontiers in Endocrinology, 13, 986616.
+ Open protocol
+ Expand
4

Histological Analysis of Murine Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine heads and femurs were stripped of soft tissues, fixed in 10% neutral buffered formalin (CellPath, Powys, United Kingdom), and decalcified in Hilleman and Lee EDTA solution (5.5% EDTA in 10% formalin) for 2 to 3 weeks. Following paraffin embedding, hematoxylin and eosin staining (Sigma-Aldrich) was performed on 5-mm brain/femur sections. Photographs were taken using an Axio Observer Light microscope (Zeiss).
Savino A.M., Fernandes S.I., Olivares O., Zemlyansky A., Cousins A., Markert E.K., Barel S., Geron I., Frishman L., Birger Y., Eckert C., Tumanov S., MacKay G., Kamphorst J.J., Herzyk P., Fernández-García J., Abramovich I., Mor I., Bardini M., Barin E., Janaki-Raman S., Cross J.R., Kharas M.G., Gottlieb E., Izraeli S, & Halsey C. (2020). Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment is dependent on Stearoyl CoA desaturase. Nature cancer, 1(10), 998-1009.
+ Open protocol
+ Expand
5

Organoid Branching Morphogenesis Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were removed from MMTV-PyMT mice and digested into small clumps of cells by collagenase, which are referred to as organoids. The organoids were cultured in Matrigel as described5 (link),46 (link) for 7 days with 1 μM compound treatment and 0.1% DMSO as control. Images were obtained using a Zeiss Axio-Observer light microscope. More than 80 organoids from each individual treatment were counted and evaluated for branching morphogenesis.
Abulwerdi F.A., Xu W., Ageeli A.A., Yonkunas M.J., Arun G., Nam H., Schneekloth JS J.r., Dayie T.K., Spector D., Baird N, & Le Grice S.F. (2019). Selective Small-Molecule Targeting of a Triple Helix Encoded by the Long Noncoding RNA, MALAT1. ACS chemical biology, 14(2), 223-235.
+ Open protocol
+ Expand
6

Immune Profiling in Melanoma Metastasis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Deparaffinization and antigen retrieval were performed in mouse melanoma lung metastasis using a PT-link system (Dako) and stained as previously described (13 (link)) The following antibodies were used for immune stainings: anti-iNOS, anti-CD206, anti-CD103, anti-Ki67, anti-granzyme B, anti-MPO, anti-CD86, anti-CD68, anti-MHC-II, anti-CD11b, anti-Ly6C, anti-Ly6G, and anti-PD-L1 all purchased from Abcam; anti-CD11c and anti-F4/80, purchased from BioLegend; anti-Foxp3 (Cell Signaling); anti-Arg1 (Bioss) and anti-CD8 (Dako) primary antibodies, anti-CD4 (BioLegend) and anti-CD25 (R&D Systems) followed by fluorescently labeled secondary antibodies. Images were acquired using an Axio Observer Light Microscope with the Apotome.2 (Zeiss). Metastatic melanoma lesions were gated by generating a region of interest (ROI), and threshold merge fluorescence was limited to ROI and calculated using the NIS-Elements Advanced Research 4.0 software (Nikon, Tokyo).
Figueiredo C.R., Azevedo R.A., Mousdell S., Resende-Lara P.T., Ireland L., Santos A., Girola N., Cunha R.L., Schmid M.C., Polonelli L., Travassos L.R, & Mielgo A. (2018). Blockade of MIF–CD74 Signalling on Macrophages and Dendritic Cells Restores the Antitumour Immune Response Against Metastatic Melanoma. Frontiers in Immunology, 9, 1132.
+ Open protocol
+ Expand
7

Evaluating 3D-Printed Scaffold Dimensions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The 3D-printed test scaffolds consisted of 15 cross-sectional layers. The first 11 layers were removed using a slicing approach, leading to the final model scaffold. The macro-dimensions (edge length and height) of the manufactured scaffolds were evaluated with an electronic vernier caliper (150 mm, Wiha, Schonach, Germany). The micro-dimensions (pore size and WRU) were visualized and measured with the Axio Observer Light Microscope (Carl Zeiss, Jena, Germany) and the affiliated software, ZEN (Carl Zeiss, Jena, Germany). Nine scaffolds per filament material were produced, and each dimension was measured three times.
Kuhlmann C., Blum J.C., Schenck T.L., Giunta R.E, & Wiggenhauser P.S. (2021). Evaluation of the Usability of a Low-Cost 3D Printer in a Tissue Engineering Approach for External Ear Reconstruction. International Journal of Molecular Sciences, 22(21), 11667.
+ Open protocol
+ Expand
8

Fluorescence Microscopy Analysis of Calcein-AM and Hoechst

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Axio Observer Light Microscope (ZEISS) microscope was used to qualitatively analyze samples for expression of Calcein-AM (green) and Hoechst (blue).
Patel R.S., Lucas J., Timmins L.M., Mukundan S., Teryek M., Bhatt R., Beaulieu A, & Parekkadan B. (2021). Non-invasive image-based cytometry for high throughput NK cell cytolysis analysis. Journal of immunological methods, 491, 112992.
+ Open protocol
+ Expand
9

Histopathological Evaluation of NAFLD in Rodents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver tissues were fixed in 4% paraformaldehyde (pH 7.4), dehydrated in a graded series of alcohol (70-100%), and embedded in paraffin. Tissue sections were made at 4 μm in adhesive slides and stained with hematoxylin/eosin. Stained slides were blindly evaluated by two veterinary pathologists (TBG and FG) using the Carl Zeiss Axio Observer light microscope equipped with Zen2 software.
Histologic evaluation took into account the main histologic features of NAFLD in rodent models [23] (link). The following parameters were considered: (1) microvesicular steatosis, (2) macrovesicular steatosis, (3) hypertrophy, (4) lobular inflammatory foci (all lymphocytic foci except for portal location), (5) portal inflammation. Microgranuloma was evaluated as well, but not used for the final score due to high similarities between groups. All parameters were evaluated in a four-level scoring system, in which 0 corresponds to absence of the alteration (< 5%), 1 to low-grade lesion (5-33% altered), 2 to moderate-grade lesion (33-66%), and 3 to high-grade lesion (> 66% altered).
Triglyceride (TG) content in the liver was determined with Triglyceride Quantification Kit (BioVision, Milpitas, USA) following the manufacturer instructions.
Stevanović-Silva J., Beleza J., Coxito P., Pereira S., Rocha H., Gaspar T.B., Gärtner F., Correia R., Martins M.J., Guimarães T., Martins S., Oliveira P.J., Ascensão A, & Magalhães J. (2021). Maternal high-fat high-sucrose diet and gestational exercise modulate hepatic fat accumulation and liver mitochondrial respiratory capacity in mothers and male offspring. Metabolism: clinical and experimental, 116.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!