Alexa fluor 594 goat anti mouse igg h l secondary antibody

Manufactured by Thermo Fisher Scientific

Alexa Fluor® 594 goat anti-mouse IgG (H+L) secondary antibody is a fluorescently labeled antibody that binds to mouse immunoglobulin (IgG) molecules. It is designed for use in immunoassays and other applications where detection of mouse primary antibodies is required.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Cytopainter phalloidin ifluor 488 reagent, by Abcam (1 mentions) Ix51 microscope, by Olympus (1 mentions) Fv1200 spectral confocal microscope, by Olympus (1 mentions) Ab76013, by Abcam (1 mentions) Alexa fluor 488 goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Ma1 38223, by Thermo Fisher Scientific (1 mentions) Cm163a, by Biocare Medical (1 mentions) Muc5ac, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor™ 594 Alexa Fluor™ 594 Goat Anti-Mouse IgG (H+L) antibody Alexa Fluor 594 goat anti-mouse IgG (H+L) Alexa Fluor 594 goat anti-mouse IgG secondary antibody Anti-Mouse IgG (H+L) Alexa Fluor 594 Alexa 594 goat anti-mouse IgG (H+L), Alexa Fluor® 594 secondary antibody (H + L; Alexa Fluor 594 goat anti-mouse secondary antibody Alexa Fluor 594 Goat Anti-Mouse IgG Antibody Goat anti-mouse IgG (H+L) secondary antibody

3 protocols using alexa fluor 594 goat anti mouse igg h l secondary antibody

Immunofluorescence Analysis of ECM Components

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MSC-deposited matrix (with and without decellularization) was fixed in 4% paraformaldehyde for 15 min and blocked in 1% bovine serum albumin (BSA) for 1 h. The samples were incubated in the appropriately diluted primary antibody against Col I or FN at 4°C overnight, followed by the Alexa Fluor^® 594 goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher Scientific) for 30 min. The cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI). Fluorescence images were obtained with an Olympus IX51 microscope (Olympus Corporation, Tokyo, Japan). To assess F-actin, UC-MSCs cultured on TCPS and ECM substrates were incubated in CytoPainter Phalloidin-iFluor 488 Reagent (Abcam) at room temperature for 1 h. After rinsing with PBS, the cell nuclei were counterstained with DAPI and fluorescent images were captured with an Olympus IX51 microscope.

Zhou L., Chen X., Liu T., Zhu C., Si M., Jargstorf J., Li M., Pan G., Gong Y., Luo Z.P., Yang H., Pei M, & He F. (2017). SIRT1-dependent anti-senescence effects of cell-deposited matrix on human umbilical cord mesenchymal stem cells. Journal of tissue engineering and regenerative medicine, 12(2), e1008-e1021.

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Detecting P-glycoprotein Expression in Tumor Tissues

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Tumor tissues were harvested from NCI/ADR-RES-bearing nude mice and were fixed in 4% paraformaldehyde overnight at 4°C. After being immersed in 30% sucrose solution overnight, tumor tissues were sectioned in 7-μm thick. To detect the Pgp expression, slides were rinsed thoroughly with PBS, then blocked in 5% Normal Goat Serum in PBS for 30 min at room temperature, blotted off serum block, and incubated with anti-Pgp C219 monoclonal antibody (Thermo Fisher Scientific) at 10 μg/mL or PBS as negative control at room temperature for 60 min. After rinsed with PBS for three times, slides were incubated with AlexaFluor-594 goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher Scientific) at 5 μg/mL at room temperature for 30 min. The slides were then rinsed with PBS twice and stained with DAPI (Thermo Fisher Scientific). The fluorescence images were taken using Olympus FV1200 Spectral Confocal Microscope (Olympus, Tokyo, Japan). A section of tumor tissue that was only stained with the secondary antibody served as control.

Wang M., Mao C., Wang H., Ling X., Wu Z., Li Z, & Ming X. (2017). Molecular Imaging of P-glycoprotein in Chemoresistant Tumors using a Dual-Modality PET/Fluorescence Probe. Molecular pharmaceutics, 14(10), 3391-3398.

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Immunofluorescence Staining of Lung Cells

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Cells were fixed with 4% paraformaldehyde (PFA) in PBS for 10 min and permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) in PBS for 5 min at room temperature. Cells were stained with the primary antibodies for 1 h and secondary antibodies for 45 min each at room temperature. DNA was counterstained with DAPI. Primary antibodies included the following: TP63 (1:100, CM163A; Biocare Medical), TTF1 (1:200, ab76013; Abcam), SOX2 (1:200, 09-0024; ReproCELL USA Inc., Beltsville, MD, USA), PE-conjugated human EpCAM (1:200, 12-9326-42; Thermo Fisher Scientific), MUC5AC (1:200, MA1-38223; Thermo Fisher Scientific), APC-conjugated human CD47 (1:20, 323123; Biolegend), PE-conjugated human CD26 (1:20, 302705; Biolegend, San Diego, CA, USA), Alexa Fluor 647-conjugated human Podoplanin (1:200, 337007; Biolegend), and Alexa Fluor 488-conjugated human SP-C (1:200, sc-518029AF488, Santa Cruz Biotechnology, Dallas, TX, USA). Secondary antibodies included Alexa Fluor 488 goat anti-mouse IgG (H+L) secondary antibody (1:200, A11029; Thermo Fisher Scientific), Alexa Fluor 568 donkey anti-rabbit IgG (H+L) secondary antibody (A10042; Thermo Fisher Scientific), and Alexa Fluor 594 goat anti-mouse IgG (H + L) secondary antibody (A11032; Thermo Fisher Scientific).

Hirai H., Liang X., Sun Y., Zhang Y., Zhang J., Chen Y.E., Mou H., Zhao Y, & Xu J. (2021). The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay. Molecular Therapy. Methods & Clinical Development, 24, 11-19.

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