Brown rice (BR) of Malaysian mixed varieties (MR219 and MR220) was procured from Padiberas Nasional (BERNAS) factory, Seri Tiram Jaya, Selangor. Food grade H2O2 and sodium hypochlorite (NaOCl) were purchased from Bendosen Laboratory Chemicals (Selangor, Malaysia) and from Dexchem Industries Sdn. Bhd. (Penang, Malaysia), respectively. Human SH-SY5Y neuroblastoma cell line was purchased from the American Type Culture Collection (ATCC), USA. Dulbecco's Modified Eagle's Medium/Nutrient F-12 mixture, fetal bovine serum, gentamicin, phosphate-buffered saline (PBS), human Aβ(1-42), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), and trypsin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Analytical grade H2O2 was purchased from Merck (Darmstadt, Germany), while CytoTox96 Non-Radioactive Cytotoxicity Assay was purchased from Promega (Southampton, UK). The GenomeLab GeXP Start Kit was purchased from Beckman Coulter, Inc. (Miami, FL, USA), and the Total RNA Isolation Kit was purchased from RBC Bioscience Corp. (Taipei, Taiwan). Magnesium chloride (MgCl2), DNA Taq polymerase, HPLC grade water, acetic acid, acetonitrile, methanol, and isopropanol were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA).
Magnesium chloride mgcl2
Manufactured by Thermo Fisher Scientific
Sourced in United States
Magnesium chloride (MgCl2) is a chemical compound that is commonly used in various laboratory applications. It is a colorless, crystalline solid that is highly soluble in water. Magnesium chloride serves as a source of magnesium ions, which are essential for many biological and chemical processes.
Automatically generated - may contain errors
Lab products found in correlation
Taq dna polymerase, by Thermo Fisher Scientific (2 mentions)
Genomelab gexp start kit, by Beckman Coulter (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Gentamicin, by Merck Group (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Hydrochloric acid (hcl), by Avantor (2 mentions)
Sulfuric acid h2so4, by Avantor (2 mentions)
L ascorbic acid, by HiMedia (2 mentions)
Glycerol, by Loba Chemie (2 mentions)
Analytical grade h2o2, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Hplc grade water, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Cytotox 96 non radioactive cytotoxicity assay, by Promega (1 mentions)
Sh sy5y human neuroblastoma cell line, by American Type Culture Collection (1 mentions)
Sh sy5y cell line, by American Type Culture Collection (1 mentions)
Thiol terminal aptamer, by NZYTech (1 mentions)
Neuraminidase na, by Merck Group (1 mentions)
Sodium arsenate dibasic heptahydrate na2haso4.7h2o, by Merck Group (1 mentions)
6 protocols using magnesium chloride mgcl2
1
Neuroprotective Effects of Malaysian Brown Rice
2
Neuroblastoma SH-SY5Y Cell Culture Protocol
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The human neuroblastoma SH-SY5Y cell line was purchased from ATCC (VA, USA). Dulbecco's Modified Essential Eagle's Medium-Ham's Nutrient Mixture F-12 (DMEM-F12), fetal bovine serum, gentamicin, and phosphate-buffered saline (PBS) were obtained from Sigma (St. Louis, MO, USA). Total RNA Isolation kit was purchased from RBC Bioscience Corp. (Taipei, Taiwan), while GenomeLab GeXP Start Kit was purchased from Beckman Coulter Inc. (Miami, FL, USA), and magnesium chloride (MgCl2) and DNA Taq polymerase were from Thermo Fisher Scientific (Pittsburgh, PA, USA).
3
Aptamer-Functionalized Gold Nanoparticles for SARS-CoV-2 Detection
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All chemicals were of analytical reagent grade and used without further purification. Double deionized (DI) water (18.6 MΩ) was used throughout the research work. Aluminium (Al) discs of 15 mm diameter were obtained from Good fellow. The thiol terminal aptamer was purchased from the Nzytech company and its sequence was: SH-(CH2)6-5′-CAG CAC CGA CCT TGT GCT TTG GGA GTG CTG GTC CAA GGG CGT TAA TGG ACA-3′ [52] (link). Gold (III) acetate Au (III) acetate, sodium borohydride (NaBH4), phosphoric acid (H3PO4), potassium hydroxide (KOH), 3-mercaptopropyl trimethoxysilane (3-MPS), oxalic acid (Ox), perchloric acid (HClO4), hydrogen peroxide (H2O2), and chromium (VI) oxide (H2CrO4), potassium ferrocyanide (Fe(CN)64−), and magnesium chloride (MgCl2) were obtained from Alfa Aesar. 3-mercapto-1-propanol (3-MCP), Tris (2-carboxyethyl) phosphine hydrochloride (TCEP), syndrome coronavirus-2 receptor-binding domain (SARS-CoV-2), hemagglutinin (HA) and neuraminidase (NA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Kapton tape (Polyimide, 100 mm wide, 33 m long) was purchased from Satkit (Tarragona, Spain).
4
Characterizing Crosslinked CNF Hydrogels
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CNFs were provided by the Process Development Center (PDC) of the University of Maine (Orono, ME, USA). The 3 wt.% CNFs were produced by mechanically refining bleached softwood Kraft pulp. This is the standard grade of CNFs produced by the PDC and contains 90% fines. In this context, the fines content indicates the percentage of fibers with lengths < 200 µm. PolycupTM (polyamide-epichlorohydrin) 5150 crosslinker (26 wt.%) was supplied by Solenis (Wilmington, DE, USA). Anhydrous ferric chloride (FeCl3; 98%) and magnesium chloride (MgCl2; 99%) were purchased from Alfa Aeser (Haverhill, MA, USA). Anhydrous ethyl alcohol (C2H5OH; 99.5%) was obtained from Acros Organics (Jair Lawn, NJ, USA). HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffering agent (≥99.5%), sodium chloride (NaCl; ≥99%), sodium hydroxide (NaOH; ≥97%), hydrochloric acid (HCl; 37%), sodium (meta) arsenite (NaAsO2), and sodium arsenate dibasic heptahydrate (Na2HAsO4.7H2O; ≥98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals and solvents were used without any further purification.
5
Enzymatic Assay of Phospholipase A2
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Adenosine 5′-triphosphate disodium salt hydrate ATP (99%), ammonium molybdate tetrahydrate (99%), dimethyl sulfoxide DMSO (99.9%), ethylenediaminetetraacetic acid disodium salt dehydrate EDTA (99%), potassium phosphate monobasic KH2PO4, and Trizma base (99%) were purchased from Sigma-Aldrich (St Louis, MO, USA). L-Ascorbic acid (99%) was provided by HiMedia (Mumbai, India). Magnesium chloride MgCl2 was furnished by Acros organics (New Jersey, NJ, USA). Hydrochloric acid HCl and sulfuric acid H2SO4 (96.3%) were both supplied by VWR chemicals. Glycerol was purchased from Loba Chemie (Mumbai, India). The natural molecules quercetin (95%) and thymoquinone (99%) were purchased from Sigma-Aldrich (St Louis, MO, USA). PLA2 and melittin from the Apis mellifera bee were purchased from Latoxan Laboratory (France).
6
Extraction and Characterization of Montivipera bornmuelleri Venom
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Extraction of the venom from Montivipera bornmuelleri snake was carried out by Dr. Riyad Sadek (American University of Beirut). The venom sample was stored at -20 • C until use.
Adenosine 5 -triphosphate disodium salt hydrate ATP (99%), ammonium molybdate tetrahydrate (99%), dimethyl sulfoxide DMSO (99%), ethylenediaminetetraacetic acid disodium salt dehydrate EDTA (99%), potassium phosphate monobasic KH 2 PO 4 , Trizma base (99%) and quercetin (95%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). L-Ascorbic acid (99%) was provided by HiMedia (Mumbai, India) and Glycerol was obtained from Loba Chemie (Mumbai, India). Magnesium chloride MgCl 2 was supplied by Acros organics (Trenton, NJ, USA). Hydrochloric acid HCl and sulfuric acid H 2 SO 4 (96.3%) were provided by VWR chemicals.
Adenosine 5 -triphosphate disodium salt hydrate ATP (99%), ammonium molybdate tetrahydrate (99%), dimethyl sulfoxide DMSO (99%), ethylenediaminetetraacetic acid disodium salt dehydrate EDTA (99%), potassium phosphate monobasic KH 2 PO 4 , Trizma base (99%) and quercetin (95%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). L-Ascorbic acid (99%) was provided by HiMedia (Mumbai, India) and Glycerol was obtained from Loba Chemie (Mumbai, India). Magnesium chloride MgCl 2 was supplied by Acros organics (Trenton, NJ, USA). Hydrochloric acid HCl and sulfuric acid H 2 SO 4 (96.3%) were provided by VWR chemicals.
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