High fat (D12492) and low fat diet (D12450B) used in this study were purchased from research diets, New Brunswick, NJ, USA. Antibodies against β-actin were purchased from Santa Cruz Biotechnology, Santa Cruz, CA, USA. Antibodies against p-Akt (Thr308), p-p70 S6 Kinase (Thr389), p70 S6 Kinase, mTOR (7C10), P-mTOR (Ser2448), p-synapsin & total Synapsin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against PA1-741 (Synapse Associated Protein 97) were purchased from Pierce (thermo scientific), Rockland, IL, and USA. Acetyl choline esterase enzyme activity kit, Amplite™ Colorimetric, was purchased from AAT Bioquest, Inc. Antibodies against collagen IV and GFAP were obtained from Abcam, Cambridge, MA, USA. Antibodies against IBA-1 were purchased from Wako, Osaka, Japan. Biotinylated secondary antibodies, DAB peroxidase substrate kit, VIP peroxidase substrate kit, horseradish peroxidase conjugated secondary antibodies, normal goat serum, normal horse serum, were purchased from vector laboratories (Burlingame, CA, USA). All immunoblotting reagents were purchased from Bio-Rad Laboratories (Hercules, CA, USA). All other items and chemicals including 10% formalin solution, phosphate buffered saline were purchased from Sigma-Aldrich chemical company.
Antibodies against iba1
Manufactured by Fujifilm
Sourced in Japan, United States
Antibodies against IBA1 are laboratory reagents used for the detection and analysis of the IBA1 protein, which is a marker for microglia, a type of immune cell found in the central nervous system. These antibodies can be utilized in various immunohistochemical and immunocytochemical techniques to visualize and study the localization and distribution of IBA1-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Neun antibody, by Merck Group (2 mentions)
Real time pcr rt pcr primers, by Qiagen (2 mentions)
Normal horse serum, by Vector Laboratories (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Vector Laboratories (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Normal goat serum (ngs), by Vector Laboratories (1 mentions)
Formalin solution, by Merck Group (1 mentions)
Dab peroxidase substrate kit, by Vector Laboratories (1 mentions)
Vip peroxidase substrate kit, by Vector Laboratories (1 mentions)
Antibodies against β actin, by Santa Cruz Biotechnology (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Tnfα elisas, by R&D Systems (1 mentions)
Gts 21, by Abcam (1 mentions)
Antibodies against β actin, by Merck Group (1 mentions)
Pparγ, by Cell Signaling Technology (1 mentions)
α bungarotoxin, by Abcam (1 mentions)
Antibodies against bcl 2, by Abcam (1 mentions)
C021 dihydrochloride c021, by Bio-Techne (1 mentions)
4 protocols using antibodies against iba1
1
Dietary Fat Modulation of Neurometabolic Pathways
2
Antibody Sources and Reagents for Neuroinflammation Study
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Fractalkine antibodies were purchased from Abcam (Cambridge, MA). Antibodies against IBA1 were purchased from Wako Chemicals USA (Richmond, VA). NeuN antibodies were ordered from Millipore (Billerica, MA). Antibodies against CD11b and glutamate aspartate transporter (GLAST) were purchased from Miltenyi Biotec (San Diego, CA). Phosphorylated and total extracellular signal-regulated kinase 1/2 (pERK1/2 and tERK1/2) antibodies were purchased from Cell Signaling (Danvers, MA). Mouse soluble fractalkine was purchased from R&D Systems (Minneapolis, MN). Mouse fracktalkine, CCL2, IL-6, and TNFα ELISAs were purchased from R&D Systems. All real-time PCR (RT-PCR) primers were purchased from SABiosciences (Frederick, MD). Total bilirubin was assayed using an ELISA kit from Cusabio (Wuha, China). All other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted and were of the highest grade available.
3
Neuroinflammation and Oxidative Stress
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GTS-21, α-bungarotoxin, and antibodies against Bcl2 and PGC-1α were purchased from Abcam (Cambridge, UK). LPS (Escherichia coli serotype 055:B5), methyllycaconitine, and antibodies against β-actin and BDNF were purchased from Sigma-Aldrich (St. Louis, MO, USA). MPTP was obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). Antibodies against Iba-1 were purchased from Wako (Osaka, Japan). Antibodies against phospho-/total forms of AMPK, Akt, MAP kinases, CREB, and antibodies for IL-6, TGF-β, NQO1, PPAR-γ, and TH were obtained from Cell Signaling Technology (Beverley, CA, USA). While antibodies against phospho-p47phox were provided by Assaybiotech (Sunnyvale, CA, USA), those against TNF-α, Nrf2, HO-1, catalase, lamin A, and COX-2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against iNOS and IL-1β were purchased from BD Biosciences (San Jose, CA, USA), and an antibody for 4-hydroxy-2E-nonenal (HNE) was purchased from Alpha Diagnostic International (San Antonio, TX, USA).
4
Antibody and Chemical Reagent Sources
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Antibodies against IBA1 were purchased from Wako Chemicals USA (Richmond, VA, USA). Antibodies against CCL2, CCR2, and CCR4 were purchased from Genetex (Irvine, CA, USA). NeuN antibodies were ordered from Millipore (Billerica, MA, USA). All real-time PCR (RT-PCR) primers were purchased from SABiosciences (Frederick, MD, USA). INCB 3284 dimesylate (INCB) and C021 dihydrochloride (C021) were purchased from Tocris Bioscience (Minneapolis, MN, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted, and were of the highest grade available.
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