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21 protocols using sodium hydroxide (naoh)

1

Chiral Analysis of Fluoxetine Enantiomers

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R,S-fluoxetine and S-fluoxetine of pharmaceutical grade were acquired from Solmag (Mulazzano, Italy). Phosphoric acid (85%), disodium hydrogenophosphate, and sodium dihydrogenophosphate were purchased from Merck (Darmstadt, Germany), and sodium hydroxide was from Lach Ner (Neratovice, Czech Republic). Deionized water was produced by a Milli-Q system (Millipore, USA). All reagents were of analytical grade.
The following CDs were used as chiral additives: native neutral CDs (β-CD, γ-CD), derivatized neutral CDs (hydroxypropyl-β-CD – HP-β-CD, randomly methylated β-CD – RAMEB, heptakis (2,6-di-O-methyl)-β-CD - DIMEB, heptakis(2,3,6-tri-O-methyl)- β-CD - TRIMEB), derivatized ionizable CDs (carboxymethyl-β-CD - CM-β-CD, sulfobuthylether-β-CD SBE-β-CD). All CDs were obtained from Cyclolab (Budapest, Hungary) with the exception of SBE-β-CD – (Capsitol®) which was obtained from Cydex Corp. (USA).
For the determination from pharmaceutical products, Prozac (Eli Lilly, USA) and Fluoxin (VimSpectrum, Romania) capsules containing 20 mg fluoxetine were used. The pharmaceutical preparations were purchased from a local pharmacy.
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2

Synthesis of Mesoporous Tin Oxide Materials

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Reagent grade chemicals were used in hard-templating and sol-gel syntheses: Pluronic F-127 (F-127, (C3H6O·C2H4O)x); Mw = 12,600 (Sigma-Aldrich Chemie GmbH, Steinheim, Germany); tetraethoxysilane (Si(OC2H5)4); TEOS, p.a. 98% (Sigma-Aldrich Chemie GmbH, Steinheim, Germany); hydrochloric acid (HCl, ≥37 wt%, Sigma-Aldrich Chemie GmbH, Steinheim, Germany); tin(II) chloride dihydrate (SnCl2·2H2O, p.a. 98+%, Acros Organics, Geel, Belgium); sodium hydroxide (NaOH, p.a. 98.9%, Lach-Ner, Czech Republic); absolute ethanol (C2H5OH, Honeywell, Seelze, Germany); and deionized water. The organic functional agents, i.e., α-terpineol (C10H18O, Sigma-Aldrich Chemie GmbH, Steinheim, Germany), ethyl-cellulose (C20H38O11,Sigma-Aldrich Chemie GmbH, Steinheim, Germany) and glacial acetic acid (C2H4O2, p.a. 99+%, Alfa Aesar, Karlsruhe, Germany) were employed, too.
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3

Gold Nanoparticle Synthesis Protocol

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Tetrachloroauric acid trihydrate (Merck Millipore, for analysis), D(+)-Maltose monohydrate (Sigma-Aldrich, p.a.), sodium hydroxide (Lach-Ner, p.a.), Tween 80 (Sigma-Aldrich, p.a.), 4-nitrophenol (Sigma-Aldrich, p.a.), sodium borohydride (Sigma-Aldrich, p.a.) were used as received without additional purification. Deionized water (18 MΩ·cm, Millipore) was used to prepare all solutions.
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4

Oxidative Stress Assay Chemicals

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The chemicals used for the oxidative stress assays were thiobarbituric acid (Carlo Erba, Val de Reuil, France, CAS 504-17-6), sodium hydroxide (Lachner, Neratovice, Czech Republic, CAS 1310-73-2), (Ethylenedinitrilo)tetraacetic acid disodium salt (Lachner, Czech Republic, CAS 6381-92-6), sulfanilic acid (Acros Organics, Geel, Belgium, CAS 121-57-3), n-1-naphthyl ethylenediamine dihydrochloride (Fisher Chemicals, Loughborough, UK, CAS 1465-25-4), sodium chloride (Lachner, Czech Republic, CAS 7647-14-5), gelatine (Acros Organics, CAS 9000-70-8), nitrotetrazolium blue chloride (Acros Organics, CAS 298-83-9), horseradish peroxidase (Sigma Aldrich, St. Louis, MO, USA, CAS 9003-99-0), Tris(hydroxymethyl)aminomethane (Acros Organics, CAS 77-86-1), Potassium dihydrogen phosphate (Lachner, Czech Republic, CAS 7778-77-0), glucose (Lachner, Czech Republic, CAS 50-99-7), phenol red (Acros Organics, CAS 143-74-8), metaphosphoric acid (Acros Organics, CAS 37267-86-0), di-sodium hydrogen phosphate (Carlo Erba, France, CAS 7558-79-4), 5,5-dithio-bis-(2-nitrobenzoic acid) (Sigma Aldrich, USA, CAS 69-78-3), trisodium citrate dihydrate (Fisher Chemicals, UK, CAS 6132-04-3), Glutathione reduced (Acros Organics, CAS 70-18-8), and L-Epinephrine (Sigma Aldrich, USA, CAS 51-43-4).
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5

Comprehensive Chemical Reagents Inventory

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The following chemicals were used: 3,5-dinitrosalicylic acid (DNSA) (98%, Thermo Fisher Scientific, Maharahstra, India), sodium sulphite (Lach-ner, Brno, Chez Republic), sodium hydroxide (Lach-ner, Brno, Chez Republic), phenol (99 + %, Thermo Fisher Scientific, Maharahstra, India), potassium sodium tartrate (Sigma-Aldrich, Buchs, Switzerland), Folin–Ciocalteu reagent (Sigma-Aldrich, Buchs, Switzerland), anhydrous sodium carbonate (Lach-ner, Brno, Chez Republic), gallic acid (anhydrous) for synthesis (Merck, Darmstadt, Germany), cellulase (from Aspergillus niger) (Sigma-Aldrich, Tokyo, Japan), pectinase (from Aspergillus niger) (Sigma-Aldrich, Buchs, Switzerland), xylanase (from Theryomyces, expressed in Aspergillus oryzae) (Sigma-Aldrich, Søborg, Denmark), beechwood xylan (Biosynth, Berkshir, England, UK), pectin from citrus peel (74.0%, Sigma-Aldrich, Buchs, Switzerland), sodium carboxymethyl cellulose (Sigma-Aldrich, Buchs, Switzerland), xylose (99%, Sigma-Aldrich, Buchs, Switzerland, D-( + )-glucose (99.5%, Sigma-Aldrich, Buchs, Switzerland), D-( + )-galacturonic acid monohydrate (97.0%, Sigma Aldrich, Buchs, Switzerland), C9-C25 alkanes, deuterated chloroform for NMR spectroscopy (CDCl3-d with 0.03% v/v TMS, 99.80%, Eurisotop, Saint-Aubin, France).
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6

Antioxidant Capacity Evaluation of Plant Extracts

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All enzymes, bile extract, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,4,6,-tris(2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ferric chloride, aluminium chloride, Folin-Ciocalteu reagent (2N), vanillin, gallic acid, quercetin, catechin, the analytical standards for UHPLC analysis (vanillic acid, ferulic acid, cinnamic acid, and naringenin, all with purity ≥ 97%), methanol Chromasolv®, and acetonitrile Chromasolv® were purchased from Sigma-Aldrich (St. Louis, MO, USA). Luteolin and apigenin standards were obtained from Thermo Fischer Scientific (Heysham, Lancashire, UK). Other inorganic salts, hydrochloric acid, and sodium hydroxide were analytical grade (Lach-Ner, Neratovice, Czech Republic).
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7

Bacterial Culture Media Preparation

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All compounds were commercial products: hydrochloric acid, sodium chloride, and sodium hydroxide (Lach-Ner, Neratovice, Czech Republic), sodium and potassium phosphates, (Penta, Praha, Czech Republic), tryptone (Oxoid, Basingstoke, UK), yeast extract, kanamycin, and tetramethoxysilane (TMOS) (Sigma-Aldrich, St. Louis, MO, USA).
Phosphate buffered saline (PBS) (pH 7.4) was prepared by dilution from a previously prepared concentrated solution (×10) of PBS that contained KH2PO4 (17 mmol/L), Na2HPO4 (52 mmol/L), and NaCl (1.5 mol/L). Luria–Bertani media (LB) contained tryptone (10 g/L), yeast extract (5 g/L), and NaCl (10 g/L), pH 7.2 [28 ]. The LB + kan50 medium was prepared by the addition of a stock kanamycin solution (10 g/L) to a final concentration of 50 mg/L after autoclaving. Minimal salts medium (MSM) contained MgSO4·7H2O (0.1 g/L), NH4NO3 (0.2 g/L), trace elements (0.1 mL), 0.5 M phosphate buffer, pH 7.2 (100 mL), and distilled water (900 mL) [27 ].
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8

Mordenite-Nickel Catalyst Synthesis for Biodiesel

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Natural mordenite was kindly provided by TECHNOTOPIA Ltd. (Athens, Greece). Hydrochloric acid solution (Carlo Erba, Emmendingen, Germany, 35% w/w), ammonium nitrate (Merck, Darmstadt, Germany) and sodium hydroxide (Lach-Ner, Neratovice, Czech Republic) were used for mordenite treatment. Nickel (II) nitrate hexahydrate (Ni(NO3)2‧6H2O) (Alfa Aesar, Ward Hill, MA, USA) and urea (CO(NH2)2) (Duchefa, Haarlem, The Netherlands) were used for catalysts synthesis. Biodiesel obtained from a local biodiesel company P. N. Pettas S.A., Edible Oils and Fats-Biofuel-Soap Manufacturers (Patras, Greece).
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9

Detailed Chemical Analysis Protocol

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All of the chemicals and reagents used in this study were of analytical grade. Ethanol, acetone, sodium carbonate (Na2CO3), and potassium sulfate (K2SO4) were purchased from Gram-mol doo (Zagreb, Croatia); sodium tetraborate, L-cystein, sulfamic acid, gelatin, potassium hydroxide, chloroform, 1-phenyl-3-methyl-5-pyrazolone (PMP), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) from Acros Organics (Geel, Belgium); acetonitrile, trichloroacetic acid (TCA), silicone oil, and Folin–Ciocalteu reagent from Fisher Scientific (Leicestershire, UK); barium chlorid (BaCl2) from abcr GmbH (Karlsruhe, Germany), fucoidan from Fucus vesiculosus, D-galacturonic acid, phenol, m-hydroxydiphenyl, 2,2-diphenyl-1-picrylhydrazyl (DPPH), arabinose, D-(+)-glucose, L-rhamnose, D-(−)-fructose, trimethylamine, 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), D-(+)-mannose, L-(−)-fucose, and ammonium acetate from Sigma-Aldrich (St. Louis, Missouri, USA); ethyl acetate, hexane, and absolute ethanol from Carlo Erba Reagents (Cornaredo, Italy); hydrochloric acid (HCl) from TKI Hrastnik (Hrastnik, Slovenia); sodium hydroxide from Lach-Ner (Zagreb, Croatia); sulfuric acid (H2SO4) from Scharlab S.L. (Barcelona, Spain); D-(−)-ribose from TCI (Portland, OR, USA); and fluorescein sodium salt from Honeywell Riedel-de-Haën (Bucharest, Romania).
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10

Functionalization of Magnetic Nanoparticles

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Ferric chloride (FeCl3), iron sulfate heptahydrate (FeSO4·7H2O), (3-aminopropyl) triethoxysilane (APTES), potassium cyanide (KCN), and phenol were purchased from Sigma Aldrich Merck (Darmstadt, Germany); sodium hydroxide (NaOH) was purchased from Lach-Ner (Tovarni, Czech Republic); ethanol and acetic acid were purchased from Emsure Merck Millipore (Darmstadt, Germany); pyridine and ninhydrin were purchased from Merck (Darmstadt, Germany). All the reagents utilized in this study were of analytical purity and used as received. Ultrapure water was used for all experiments.
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