The following chemicals were used: 3,5-dinitrosalicylic acid (DNSA) (98%, Thermo Fisher Scientific, Maharahstra, India), sodium sulphite (Lach-ner, Brno, Chez Republic),
sodium hydroxide (Lach-ner, Brno, Chez Republic),
phenol (99 + %, Thermo Fisher Scientific, Maharahstra, India),
potassium sodium tartrate (Sigma-Aldrich, Buchs, Switzerland),
Folin–Ciocalteu reagent (Sigma-Aldrich, Buchs, Switzerland), anhydrous sodium carbonate (Lach-ner, Brno, Chez Republic), gallic acid (anhydrous) for synthesis (Merck, Darmstadt, Germany), cellulase (from
Aspergillus niger) (Sigma-Aldrich, Tokyo, Japan), pectinase (from
Aspergillus niger) (Sigma-Aldrich, Buchs, Switzerland), xylanase (from
Theryomyces, expressed in
Aspergillus oryzae) (Sigma-Aldrich, Søborg, Denmark),
beechwood xylan (Biosynth, Berkshir, England, UK),
pectin from citrus peel (74.0%, Sigma-Aldrich, Buchs, Switzerland),
sodium carboxymethyl cellulose (Sigma-Aldrich, Buchs, Switzerland),
xylose (99%, Sigma-Aldrich, Buchs, Switzerland,
D-( + )-glucose (99.5%, Sigma-Aldrich, Buchs, Switzerland),
D-( + )-galacturonic acid monohydrate (97.0%, Sigma Aldrich, Buchs, Switzerland), C
9-C
25 alkanes, deuterated chloroform for NMR spectroscopy (CDCl
3-d with 0.03%
v/v TMS, 99.80%, Eurisotop, Saint-Aubin, France).
Miljanović A., Bielen A., Grbin D., Marijanović Z., Andlar M., Rezić T., Roca S., Jerković I., Vikić-Topić D, & Dent M. (2020). Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Chemical Composition of Essential Oils. Molecules, 25(20), 4818.