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Fk506 prograf

Manufactured by Astellas Pharma
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Sourced in United States

FK506, also known as Prograf, is a laboratory-produced compound used in research and clinical applications. It functions as an immunosuppressant agent, primarily utilized in organ transplant procedures to prevent rejection. The core function of FK506 is to inhibit the activation and proliferation of T cells, a key component of the immune system.

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Lab products found in correlation

Sunflower oil, by Merck Group (2 mentions) Cyclosporin a csa, by Merck Group (2 mentions)

Close spelling variants of this product

Prograf (77 citations) FK506 (14 citations)

2 protocols using fk506 prograf

1

Investigating LCMV Infection and Immune Modulation

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LCMV strains Armstrong (Arm) and clone-13 (Cl-13) were propagated and titers were determined as previously described53 . C57BL/6J mice were infected intraperitoneally (i.p.) with 2×105 plaque-forming units (PFU) of LCMV Arm or intravenously (i.v.) with 4×106 PFU LMCV Cl-13. For other experiments, mice were infected with 2×106 PFU VSV-OVA (i.v.) or 1×104 colony-forming units (CFU) LM-GP33 (i.p.). For influenza infection, mice were anesthetized with isofluorane and ketamine prior to intranasal administration of 50 TCID50 PR8-GP33 (H1N1 strain) in 30μl of PBS. FK506 (Prograf, Astellas Pharma US) was prepared for injection by diluting to 1.5mg/ml in PBS. Diluted FK506 was administered s.c. at a dose of 10mg/kg from d3–7 or d25–29 of LCMV Cl-13 infection54 . Cyclosporin A (CsA, Sigma-Aldrich) was prepared by diluting in sunflower oil (Sigma-Aldrich). 40mg/kg of diluted CsA was administered IP daily for duration of treatment. For control treatments, PBS was administered s.c.
Khan O., Giles J.R., McDonald S., Manne S., Ngiow S.F., Patel K.P., Werner M.T., Huang A.C., Alexander K.A., Wu J.E., Attanasio J., Yan P., George S.M., Bengsch B., Staupe R.P., Donahue G., Xu W., Amaravadi R.K., Xu X., Karakousis G.C., Mitchell T.C., Schuchter L.M., Kaye J., Berger S.L, & Wherry E.J. (2019). TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature, 571(7764), 211-218.
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2

Investigating LCMV Infection and Immune Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
LCMV strains Armstrong (Arm) and clone-13 (Cl-13) were propagated and titers were determined as previously described53 . C57BL/6J mice were infected intraperitoneally (i.p.) with 2×105 plaque-forming units (PFU) of LCMV Arm or intravenously (i.v.) with 4×106 PFU LMCV Cl-13. For other experiments, mice were infected with 2×106 PFU VSV-OVA (i.v.) or 1×104 colony-forming units (CFU) LM-GP33 (i.p.). For influenza infection, mice were anesthetized with isofluorane and ketamine prior to intranasal administration of 50 TCID50 PR8-GP33 (H1N1 strain) in 30μl of PBS. FK506 (Prograf, Astellas Pharma US) was prepared for injection by diluting to 1.5mg/ml in PBS. Diluted FK506 was administered s.c. at a dose of 10mg/kg from d3–7 or d25–29 of LCMV Cl-13 infection54 . Cyclosporin A (CsA, Sigma-Aldrich) was prepared by diluting in sunflower oil (Sigma-Aldrich). 40mg/kg of diluted CsA was administered IP daily for duration of treatment. For control treatments, PBS was administered s.c.
Khan O., Giles J.R., McDonald S., Manne S., Ngiow S.F., Patel K.P., Werner M.T., Huang A.C., Alexander K.A., Wu J.E., Attanasio J., Yan P., George S.M., Bengsch B., Staupe R.P., Donahue G., Xu W., Amaravadi R.K., Xu X., Karakousis G.C., Mitchell T.C., Schuchter L.M., Kaye J., Berger S.L, & Wherry E.J. (2019). TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature, 571(7764), 211-218.
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