After 3 days treatment with lentiviral control or IER3IP1 shRNA, MIN6 cells were fixed for 15 min in 4% paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS for 5 min and then incubated for 1 h in a 5% BSA/PBS blocking solution. Then cells were incubated for overnight at 4°C with a mouse monoclonal anti-cytochrome c IgG (Pharmingen) followed by exposure to a goat anti-mouse Alexa488-conjugated secondary antibody (invitrogen). Images were obtained using an Evos microscope (Advanced microscope group). The release of cytochrome C was measured by ELISA kit (Abcam).
Mouse monoclonal anti cytochrome c igg
Manufactured by BD
The mouse monoclonal anti-cytochrome c IgG is a laboratory reagent used to detect and quantify the presence of cytochrome c in biological samples. It is a purified immunoglobulin G (IgG) antibody produced by mouse hybridoma cells that specifically binds to cytochrome c, a heme-containing protein involved in cellular respiration.
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2 protocols using mouse monoclonal anti cytochrome c igg
1
Cytochrome C Release Quantification
2
Cytochrome c Immunofluorescence in MIN6 Cells
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After 4 days of treatment with lentiviral control or Pdx1 shRNA, MIN6 cells were fixed for 15 min in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS for 5 min and then incubated for 1 h in a 5% BSA/PBS blocking solution. Then cells were incubated overnight at 4 °C with a mouse monoclonal anti-cytochrome c IgG (Pharmingen) followed by exposure to a goat anti-mouse Alexa488-conjugated secondary antibody (Invitrogen). Images were obtained on an Evos microscope (Advanced Microscopy Group).
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