Antibody against hif 1α

Manufactured by BD

Sourced in United States

The Antibody against HIF-1α is a laboratory reagent designed to detect and quantify the hypoxia-inducible factor-1 alpha (HIF-1α) protein. HIF-1α is a transcription factor that plays a crucial role in the cellular response to low oxygen conditions. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), to measure HIF-1α expression levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin, by Abcam (3 mentions) Chemiluminescence system, by Merck Group (2 mentions) Anti glut 1 ab40084, by Abcam (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Merck Group (2 mentions) Rhein, by Merck Group (1 mentions) Cell culture materials, by Thermo Fisher Scientific (1 mentions) Anti slug antibody, by Cell Signaling Technology (1 mentions) Phospho gsk3β ser9, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg, by Merck Group (1 mentions) Emodin, by Merck Group (1 mentions) Anti snail, by Cell Signaling Technology (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions) Gefitinib, by Abcam (1 mentions) Erlotinib, by Abcam (1 mentions) Canertinib, by Abcam (1 mentions) Bicalutamide, by Merck Group (1 mentions) Enzalutamide, by Merck Group (1 mentions) Antibodies against anxa6, by Santa Cruz Biotechnology (1 mentions) Antibody against β actin actb, by Merck Group (1 mentions) Lapatinib ditosylate, by Abcam (1 mentions)

9 protocols using antibody against hif 1α

Emodin, AE, and Rhein Modulation of EMT

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture materials were obtained from Invitrogen (Burlington, Ontario, Canada). The reagents 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), propidium iodide (PI) and the PureLink™ HiPure Plasmid DNA Purification Kit were purchased from Sigma (St. Louis, MO, USA). Primary antibodies against PARP and Twist were purchased from GeneTex (Beverly, MA, USA). Anti-HER-2, anti-phospho-Akt (Ser473), anti-E-cadherin, anti-vimentin, anti-Snail, and anti-Slug antibodies were purchased from Cell Signaling (Beverly, MA, USA). Primary antibodies against ILK, phosphor-ILK (Thr173), phospho-mTOR (Ser2448), and phospho-GSK3β (Ser9) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against YB-1 and β-Actin were purchased from Millipore (Temecula, CA, USA). The antibody against HIF-1α was purchased from BD Biosciences Clontech (San Jose, CA, USA). For Western blotting, the secondary antibodies of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were obtained from Millipore (Temecula, CA, USA), and enhanced chemiluminescence (ECL) reagents were purchased from Sigma-Aldrich. Emodin, AE, and rhein were purchased from Sigma-Aldrich. The RNAi Consortium of YBX-1 was selected by the National RNAi Core Facility.

Ma J.W., Hung C.M., Lin Y.C., Ho C.T., Kao J.Y, & Way T.D. (2016). Aloe-emodin inhibits HER-2 expression through the downregulation of Y-box binding protein-1 in HER-2-overexpressing human breast cancer cells. Oncotarget, 7(37), 58915-58930.

+ Open protocol

+ Expand

Antibody-based Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against AnxA6 as well as secondary anti-mouse, anti-goat, and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody against HIF-1α was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Antibodies against HIF-2α and EGFR, were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against β-actin (ACTB) was purchased from Sigma Aldrich (St. Louis, MO, USA). Glucose uptake assay kit (Fluorometric; ab136956) and ATP Assay Kit (Colorimetric/Fluorometric; ab83355) were purchased from Abcam (Cambridge, UK). A set of EGFR-TKIs including lapatinib-ditosylate, erlotinib, gefitinib, and canertinib were purchased from BioVision Inc. (Milpitas, CA, USA). The AR antagonists bicalutamide and enzalutamide were purchased from Sigma Aldrich (St. Louis, MO, USA). Except otherwise indicated, all other reagents were purchased from Sigma Aldrich and/or Thermo Fisher Scientific (Waltham, MA, USA).

Williams S.D., Smith T.M., Stewart L.V, & Sakwe A.M. (2022). Hypoxia-Inducible Expression of Annexin A6 Enhances the Resistance of Triple-Negative Breast Cancer Cells to EGFR and AR Antagonists. Cells, 11(19), 3007.

+ Open protocol

+ Expand

Interleukin-6 Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human interleukin-6 (IL6) protein was from R&D Systems (Minneapolis, MN, USA); the MEK1/2 inhibitor U0126, the NFκB inhibitor BAY 11-7085, dexamethasone, doxycycline, 4-hydroxytamoxifen (4-OHT), and DMSO were from Sigma (St Louis, MO, USA). Antibodies against JunB (C-11: Cat# sc-8051, RRID:AB_2130023) and extracellular signal-regulated kinase 2/ERK2 (D-2: Cat# sc-1647, RRID:AB_627547) were obtained from Santa Cruz Biotechnology (Heidelberg, Germany), and the antibody against Hif-1α (Cat# 565924, RRID:AB_2739388) from BD Biosciences (Heidelberg, Germany).

Fan F., Malvestiti S., Vallet S., Lind J., Garcia-Manteiga J.M., Morelli E., Jiang Q., Seckinger A., Hose D., Goldschmidt H., Stadlbauer A., Sun C., Mei H., Pecherstorfer M., Bakiri L., Wagner E.F., Tonon G., Sattler M., Hu Y., Tassone P., Jaeger D, & Podar K. (2021). JunB is a key regulator of multiple myeloma bone marrow angiogenesis. Leukemia, 35(12), 3509-3525.

+ Open protocol

+ Expand

Western Blotting Analyses of Cell Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analyses of western blotting were performed as previously described [19 (link)].
Antibodies against p-Rb^Ser780 (#9307), Rb (#9309), cyclin D1 (#2926), CDK6 (#3136), c-myc (#9402), p-AKT^Ser473 (#9271), AKT (#9272), p-mTOR^Ser2448 (#2971), mTOR (#2972), p-ERK1/2^{Thr202/Tyr204} (#4370), ERK1/2 (#4695), were from CST (Danvers, MA); anti-p-CDK6^Tyr24 (sc-293,097) was from Santa Cruz Biotechnology, Incorporated (Dallas, TX). Anti CDKN2A/p16^INK4a (ab81278) and anti-GLUT-1 (ab40084) were from Abcam (Cambridge, UK). Antibody against HIF-1α (#610959) was from BD Biosciences (Franklin Lakes, NJ). Anti-β-actin (#3598) was from BioVision (Milpitas, CA). All the antibodies were used at the recommended dilution of 1:1000. Horseradish peroxidase-conjugated secondary antibodies (1:10,000) and chemiluminescence system were from Millipore (Millipore, MA). Reagents for electrophoresis and blotting analysis were from BIO-RAD Laboratories (Hercules, CA).

Cretella D., Ravelli A., Fumarola C., La Monica S., Digiacomo G., Cavazzoni A., Alfieri R., Biondi A., Generali D., Bonelli M, & Petronini P.G. (2018). The anti-tumor efficacy of CDK4/6 inhibition is enhanced by the combination with PI3K/AKT/mTOR inhibitors through impairment of glucose metabolism in TNBC cells. Journal of Experimental & Clinical Cancer Research : CR, 37, 72.

+ Open protocol

+ Expand

Antibody-based EMMPRIN Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibody directed against EMMPRIN was obtained from Santa Cruz Biotechnology (TX, USA). Antibody against HIF-1α was purchased from BD (BD Pharmingen, CA, USA). Antibodies of anti-CD44, CD24, Y705-phosphorylated STAT3 (STAT3-Y705), and total STAT3 were obtained from Cell Signaling Technology. All other chemicals were purchased from Sigma-Aldrich. Recombinant human EMMPRIN was purchased from R&D (Minneapolis, MN, USA).

Liu Y., Zhang J., Sun X, & Li M. (2016). EMMPRIN Down-regulating miR-106a/b Modifies Breast Cancer Stem-like Cell Properties via Interaction with Fibroblasts Through STAT3 and HIF-1α. Scientific Reports, 6, 28329.

+ Open protocol

+ Expand

Protein Analysis by 1-D PAGE

Check if the same lab product or an alternative is used in the 5 most similar protocols

Procedures for protein extraction, solubilization, and protein analysis by 1-D PAGE are described elsewhere [22 (link)]. Antibodies against p-FGFR^Tyr653/654, FGFR1, p-FRS2-α^Tyr196, p-ERK1/2^{Thr202/Tyr204}, ERK1/2, p-mTOR^Ser2448, mTOR, p-AKT^ser473, AKT, p-P70S6K^Thr389, P70S6K, p-AMPKα1^Thr172, p-src^Tyr416, src, p-FAK^Tyr397, FAK were from Cell Signaling Technology (Beverly, MA); the antibodies against GLUT-1 and AMPKα1 were from AbCam (Cambridge, MA); the antibody against HIF-1α was from BD Biosciences (Franklin Lakes, NJ); the antibody against actin was from Sigma-Aldrich. HRP-conjugated secondary antibodies were from Pierce (Rockford, IL) and chemiluminescence system (ImmobilionTM Western Chemiluminescent HRP Substrate) was from Millipore (Temecula, CA).

Fumarola C., Cretella D., La Monica S., Bonelli M.A., Alfieri R., Caffarra C., Quaini F., Madeddu D., Falco A., Cavazzoni A., Digiacomo G., Mazzaschi G., Vivo V., Barocelli E., Tiseo M., Petronini P.G, & Ardizzoni A. (2017). Enhancement of the anti-tumor activity of FGFR1 inhibition in squamous cell lung cancer by targeting downstream signaling involved in glucose metabolism. Oncotarget, 8(54), 91841-91859.

+ Open protocol

+ Expand

Western Blotting Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The procedures for protein extraction, solubilization, and protein analysis by western blotting are described elsewhere^{39 (link)}. Antibodies against p-Rb^Ser780 (#9307), c-Myc (#9402), PARP-1 (#9532), p-AKT^Ser473 (#9271), AKT (#9272), p-mTOR^Ser2448 (#2971), mTOR (#2972), were from CST (Danvers, MA); anti-GLUT-1 (ab40084) was from Abcam (Cambridge, UK). Antibody against HIF-1α (#610959) was from BD Biosciences (Franklin Lakes, NJ). Anti-β-actin was from BioVision (#3598) (Milpitas, CA). Horseradish peroxidase-conjugated secondary antibodies and chemiluminescence system were from Millipore (Millipore, MA). Reagents for electrophoresis and blotting analysis were from BIO-RAD Laboratories (Hercules, CA). The chemiluminescent signal was acquired by C-DiGit® Blot Scanner and the spots were quantified by Image Studio™ Software, LI-COR Biotechnology (Lincoln, NE).

Cretella D., Fumarola C., Bonelli M., Alfieri R., La Monica S., Digiacomo G., Cavazzoni A., Galetti M., Generali D, & Petronini P.G. (2019). Pre-treatment with the CDK4/6 inhibitor palbociclib improves the efficacy of paclitaxel in TNBC cells. Scientific Reports, 9, 13014.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein extraction, solubilization, and protein analysis by Western blotting were performed as described [39 (link)]. Antibodies against p-Rb^Ser780, Rb, c-myc, PTEN, p-AKT^Ser473, AKT, p-4E-BP1^Thr37/46, 4E-BP1 were from CST (Danvers, MA, USA); anti-GLUT-1 was from Abcam (Cambridge, UK). The antibody against HIF-1α was from BD Biosciences (Franklin Lakes, NJ, USA). Anti-β-actin was from BioVision (Milpitas, CA, USA). The procedure for GLUT-1 detection was previously described [40 (link)]. Horseradish peroxidase-conjugated secondary antibodies and chemiluminescence system were from Millipore (Millipore, MA, USA). Reagents for electrophoresis and blotting analysis were from BIO-RAD Laboratories (Hercules, CA, USA). The chemiluminescent signal was acquired by C-DiGit^®. Blot Scanner and the spots were quantified by Image Studio^TM Software, LI-COR Biotechnology (Lincoln, NE, USA).

Bonelli M., Terenziani R., Zoppi S., Fumarola C., La Monica S., Cretella D., Alfieri R., Cavazzoni A., Digiacomo G., Galetti M, & Petronini P.G. (2020). Dual Inhibition of CDK4/6 and PI3K/AKT/mTOR Signaling Impairs Energy Metabolism in MPM Cancer Cells. International Journal of Molecular Sciences, 21(14), 5165.

+ Open protocol

+ Expand

HeLa and HUVEC Cell Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco) supplemented with antibiotics (100 U/ml of penicillin and 100 μg/ml streptomycin; Invitrogen Life Technologies, Carlsbad, CA, USA). Human umbilical venous endothelial cells (HUVECs) were grown for 4-8 passages in human endothelial-serum free medium (SFM, Gibco) with 10% FBS. Hypoxic culture was achieved by treatment with cobalt chloride (CoCl₂), a hypoxia-mimicking agent, for 12-16 h. In some cases, hypoxic cells were kept in a gas-controlled chamber (Thermo Fisher Scientific Inc., Waltham, MA, USA) at 1% O₂, 94% N₂, and 5% CO₂. DAA was purchased from ABI Chem (Munchem, Germany) and dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St Louis, MO, USA). Cycloheximide (CHX) and CoCl₂ were obtained from Sigma-Aldrich Co. Hygromycin and puromycin were purchased from Invitrogen Life Technologies. Primary antibodies against phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199), phospho-Akt (Ser473), phospho-p44/p42 MAPK (Erk1/2; Thr202/Tyr204), Akt, and Erk1/2 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against HIF-1α was obtained from BD Biosciences (San Diego, CA, USA). All other antibodies including those against actin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Choi S.W., Lee K.S., Lee J.H., Kang H.J., Lee M.J., Kim H.Y., Park K.I., Kim S.L., Shin H.K, & Seo W.D. (2016). Suppression of Akt-HIF-1α signaling axis by diacetyl atractylodiol inhibits hypoxia-induced angiogenesis. BMB Reports, 49(9), 508-513.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!