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Cd5 pe

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The CD5-PE is a fluorescently-labeled monoclonal antibody that binds to the CD5 antigen on the surface of T cells and a subset of B cells. It is designed for use in flow cytometry applications to identify and quantify CD5-positive cell populations.

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Lab products found in correlation

Cd19 fitc, by BD (1 mentions) Hema 3, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Cd21 fitc, by BioLegend (1 mentions) Igm apc, by BioLegend (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) Gl7 fitc, by BioLegend (1 mentions) Cd138 pe, by BioLegend (1 mentions) B220 alexa700, by BioLegend (1 mentions) Cd23 pe cy7, by BioLegend (1 mentions) Cd95 apc, by Thermo Fisher Scientific (1 mentions) Cd25 apc, by Thermo Fisher Scientific (1 mentions) Cd4 fitc, by Thermo Fisher Scientific (1 mentions) Cd44 apc, by Thermo Fisher Scientific (1 mentions) Cd8 fitc, by Thermo Fisher Scientific (1 mentions) B220 fitc, by Thermo Fisher Scientific (1 mentions) Cd23 apc, by Thermo Fisher Scientific (1 mentions) Cd62l pe, by Thermo Fisher Scientific (1 mentions) Cxcr3 apc, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-human CD5 (UCHT2)-PE (4 citations) PE-Cy7 Anti-Mouse-CD5 (53-7.3) (3 citations) PE-conjugated anti-canine CD5 (3 citations) CD5-PE/Cy7 (3 citations) PE-anti-CD5 (clone UCHT2) (2 citations)

3 protocols using cd5 pe

1

Murine Hematological Characterization

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Blood collected from heart puncture was analyzed by Dept. of Veterinary Medicine hematology core at MD Anderson Cancer Center. Blood smear slides were stained with Hema-3 fixative (Fisher Healthcare) and microscopically analyzed by a hematopathologist. Splenocytes isolated from wild type, 2MX-S and 8MX-S mouse spleens were treated with RBC lysis buffer and labelled with CD19-FITC (BD Pharmingen) and CD5-PE (eBiosciences) antibodies in binding buffer. Flow analysis was carried out by flow cytometry core at MD Anderson Cancer Center.
Pant V., Larsson C.A., Aryal N., Xiong S., You M.J., Quintas-Cardama A, & Lozano G. (2017). Tumorigenesis promotes Mdm4-S overexpression. Oncotarget, 8(16), 25837-25847.
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2

Identification of B Cell Subsets in Mice

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To identify the different B cell populations, two stains were performed in splenocytes from 4 to 5.5 month old mice (2 females and 1 male wt, and 4 females Kmt2d−/−). First, to identify transitional, follicular and marginal zone populations, cells were stained with CD21-FITC (Biolegend, clone 7E9, #123407), CD5-PE (eBioscience, clone 53–7.3 #12-0051-81), CD23-PECY7 (Biolegend, clone B3B4 #101613), IgM-APC (Biolegend, clone RMM-1 #406509), and B220-Alexa700 (Biolegend, clone RA3 #103232). To identify intermediate plasma cells/plasmablast (IPC), plasma cells (PC) and germinal center populations, cells were stained with GL7-FITC (Biolegend, clone GL7 #144003), CD138-PE (Biolegend, clone 281-2 #142503), CD95-APC (eBioscience, clone 15A7 #17-0951-80), B220-Alexa700 (Biolegend, clone RA3 #103232). To determine the percentages of cell populations, values were normalized by % B220+ single live cells (single cells, 7-AAD negative. B220+). 7-AAD (Life Technologies) was used to identify dead cells. Data acquisition was performed in a BD LSR II Flow Cytometer (BD Biosciences) and analysis was performed with FlowJo software (Tree Star).
Ortega-Molina A., Boss I.W., Canela A., Pan H., Jiang Y., Zhao C., Jiang M., Hu D., Agirre X., Niesvizky I., Lee J.E., Chen H.T., Ennishi D., Scott D.W., Mottok A., Hother C., Liu S., Cao X.J., Tam W., Shaknovich R., Garcia B.A., Gascoyne R.D., Ge K., Shilatifard A., Elemento O., Nussenzweig A., Melnick A.M, & Wendel H.G. (2015). The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development. Nature medicine, 21(10), 1199-1208.
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3

Lymphocyte Immunophenotyping by Flow Cytometry

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Lymphocyte stainings were performed by incubation for 20 min on ice using conjugated the following mAbs (eBioscience, San Diego, CA, USA): CD8-FITC (#11-0081-82), CD4-FITC(#MCD0401), CXCR3-APC (#17-1831-82), CCR5-PE (#12-1951-81), CD62L-PE (#12-0621-81), CD44-APC (#17-0441-81), CD25-APC (#17-0251-82), B220 FITC (#11-0452-82), CD5-PE (#12-0051-82), CD23-APC (#MCD2305).
Baur J., Otto C., Steger U., Klein-Hessling S., Muhammad K., Pusch T., Murti K., Wismer R., Germer C.T., Klein I., Müller N., Serfling E, & Avots A. (2018). The Transcription Factor NFATc1 Supports the Rejection of Heterotopic Heart Allografts. Frontiers in Immunology, 9, 1338.
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