Hrp labeled goat anti rat igg h l
HRP-labeled goat anti-rat IgG (H + L) is a laboratory reagent used for immunodetection and quantification applications. It is composed of polyclonal goat antibodies that bind to rat immunoglobulin G (IgG) molecules, both the heavy and light chains. These antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that can be used for colorimetric or chemiluminescent detection.
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3 protocols using hrp labeled goat anti rat igg h l
Protein-Protein Interaction Study via Co-Immunoprecipitation
Polyclonal Antibody Generation against rCgDM9CP-4
After SDS-PAGE, the samples of rCgDM9CP-4 were electrophoretically transferred onto a nitrocellulose membrane. The membrane was blocked with PBST containing 5% milk at room temperature for 1 h, and incubated with antibodies (diluted at 1:4000) for 1 h at room temperature with rocking, followed by three times of washes with PBST. Membranes were incubated with HRP-labeled Goat Anti-Rat IgG(H+L) (Beyotime; 1:10000) for 1 h at room temperature with rocking, followed by three times of washes with PBST. Membranes were incubated briefly in Western lighting ECL substrate system (Thermo Scientific, USA) before exposure to KODAK X-OMAT AR X-ray film (Eastman Kodak, USA).
Tissue Histology Analysis by H&E, Iron, and IHC
The frozen tissues were sliced into sections of 10 μm each at −20 °C and fixed for 10 min in 4% paraformaldehyde. Tissue sections were staining with Perls’ Iron Stain Kit (Solarbio, Beijing, China) following manufacturer’s instructions. When indicated, Perls’ staining was further enhanced using the DAB-kit (Beyotime, Shanghai, China). For immunohistochemistry, tissue sections were fixed, washed in PBS and treated with H2O2 to block endogenous peroxidase. Sections were incubated with anti-F4/80 (clone BM8, Biolegend, San Diego, CA, USA, 1:500) at 4 ℃ overnight and were incubated with HRP-labeled Goat Anti-Rat IgG (H + L) (Beyotime, Shanghai, China, 1:100) at 37 °C for 1 h and then stained with diaminobenzidine (DAB) at room temperature for 10 min in the dark, followed by counterstaining with hematoxylin for cell nuclei. Images were acquired with a Leica microscope.
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