Pulverized pancreatic tissue was resuspended in lysis buffer (Raybiotech, #AA-LYS-10ml) with protease/phosphatase inhibitors and the proteins were extracted. Solution of 1μg/μl was applied to pancreatic-amylase strips (Roche, #11126679202) and loaded to Reflotron® machine.
Pancreatic amylase strips
Manufactured by Roche
Pancreatic-amylase strips are a laboratory diagnostic tool used to detect and measure the levels of the enzyme pancreatic amylase in biological samples. Pancreatic amylase is an important marker for the evaluation of pancreatic function and the diagnosis of pancreatic disorders.
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Lab products found in correlation
2 protocols using pancreatic amylase strips
1
Pancreatic Amylase Extraction and Assay
2
Serum Amylase and Lipase Evaluation
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To determine the serum amylase activity, blood was collected from the animals at the indicated time points. Amylase activities were measured using the Reflotron Analyzer with the pancreatic amylase strips (Roche #11200658). Serum lipase activity was determined by the Lipase-PSTM kit (Trinity Biotech #805 A) according to the manual provided by the manufacturer.
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