Hcl 1m

Manufactured by Merck Group

Sourced in Italy

HCl 1M is a laboratory solution containing 1 molar concentration of hydrochloric acid. It is a commonly used reagent in various scientific and analytical applications.

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Lab products found in correlation

5 protocols using hcl 1m

Hydrogel Synthesis with Glycine, Cystine, and Acrylamide

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Glycine (coded as G, 98%), L-cystine (C, >98.0%), N,N′-methylenebisacrylamide (M, 99%), lithium hydroxide monohydrate (LiOH^.H₂O, 98%), and HCl 1M (aqueous solution), were supplied by Sigma-Aldrich (Milano, Italy) and used as received.
Cotton (COT) with an area density of 200 g m⁻² was purchased from Fratelli Ballesio S.r.l. (Torino, Italy).

Beduini A., Carosio F., Ferruti P., Ranucci E, & Alongi J. (2019). Sulfur-Based Copolymeric Polyamidoamines as Efficient Flame-Retardants for Cotton. Polymers, 11(11), 1904.

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Synthesis of Hydrogel Biomaterials

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Glycine (coded as GLY, 98%), L-alanine (ALA, 99%), L-valine (VAL, >99%), L-leucine (LEU, >98%), L-histidine (HIS, >99%), L-serine (SER, 99%), L-asparagine (ASN, >98%), L-glutamine (GLN, 99%), L-aspartic acid (ASP, >99%), L-glutamic acid monohydrate (GLU, >98%), N,N′-methylenebisacrylamide (MBA, 99%), lithium hydroxide monohydrate (98%), and HCl 1M (aqueous solution), were supplied by Sigma-Aldrich (Milano, Italy) and used as received.
Cotton (COT) with an area density of 240 gm⁻² was purchased from Fratelli Ballesio S.r.l. (Torino, Italy).

Beduini A., Carosio F., Ferruti P., Ranucci E, & Alongi J. (2021). Polyamidoamines Derived from Natural α-Amino Acids as Effective Flame Retardants for Cotton. Polymers, 13(21), 3714.

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Quantification of Human IgG Levels

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The IgG levels were determined in all plasma samples using an in-house ELISA. Briefly, MaxiSorp 96-well-plates (Nunc, Rochester, NY, USA) were coated with PBS containing 1.5 μg/ml of anti-human IgG (A0170, Sigma). After overnight incubation at 4°C, the plates were then washed three times with phosphate-buffered saline-0.05% Tween 20 (PBS-Tween) and blocked for 1 h at 37°C with PBS-Tween containing 5% nonfat-dried milk (PBS-Tween-M). Plasma samples diluted 1:1000 in PBS-Tween-M were added in duplicate wells containing 5% nonfat-dried milk (PBS-Tween-M) were added in duplicate wells. After 1 h at 37°C and three washings, specific antibodies were detected by goat peroxidase-conjugated anti-human IgG (Sigma, St. Louis) and followed by the addition of 3,3′,5,5′-tetramethylbenzidine (TMB) for 30 minutes. The reaction was stopped with HCl 1M (Merck) and optical density was measured at 450 nm using a SpectraMax microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The IgG level in each sample was calculated interpolating the mean optical density value of the sample on a linear regression graphic of recombinant IgG (ref. 15260) standard curve dilution (ranging from 0.500 to 0.031 µg/mL) performed using the same conditions described above (27 (link)).

Nogueira O.C., Gandini M., Cabral N., de Figueiredo V., Rodrigues-da-Silva R.N., Lima-Junior J.D., Pinheiro R.O., Pereira G.M., Pessolani M.C, & de Macedo C.S. (2021). Changes in B Cell Pool of Patients With Multibacillary Leprosy: Diminished Memory B Cell and Enhanced Mature B in Peripheral Blood. Frontiers in Immunology, 12, 727580.

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Radiosynthesis and Purification of [18F]FHBG

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[¹⁸F]FHBG synthesis was adapted from the method described previously [3 (link)]. Briefly, [¹⁸F]FHBG was produced on a Raytest® synthesis module by nucleophilic substitution using tosyl-FHBG (ABX). as a precursor. [¹⁸F]Fluoride is produced by the cyclotron (Cyclone 10/5 IBA) via the ¹⁸O (p, n) ¹⁸F reaction. After azeotropic drying, the precursor was heated for 20 min at 110°C. The reaction mixture was then cooled and added to the hydrolysis solution (HCl 1M, Merck) and heated for 5 min at 115°C. The reaction mixture was then neutralised by adding 2 M NaOH and 0.5 M trisodium citrate solutions (Cooper). HPLC purification was carried out in a semi-prepared Bischoff column (Prontosil®, L 250 mm, ø 10 mm, pores 5 μm) with a mobile phase consisting of absolute ethanol (WRS pharma prolabo) /sodium acetate (0.1M, Cooper) mixture (10/90 v/v). The [¹⁸F]FHBG retention time was 17.52 min, with a flow rate of 2 ml/m. Specific activity was superior to 3.2 GBq/μmol. 100% of radiochemical purity was not observed until 4 h after radiosynthesis. Radioactivity concentration was always superior to 350 MBq/ml with a low quantity of ethanol (< 10%) for experimental use on biological materials (in-vitro cell incubation or animal experiments). After medium dilution, [¹⁸F]FHBG incubation medium for cellular uptake always contains less than 0.001% of ethanol.

Salabert A.S., Vaysse L., Beaurain M., Alonso M., Arribarat G., Lotterie J.A., Loubinoux I., Tafani M, & Payoux P. (2017). Imaging grafted cells with [18F]FHBG using an optimized HSV1-TK mammalian expression vector in a brain injury rodent model. PLoS ONE, 12(9), e0184630.

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Standardized Anthocyanin Quantification Protocol

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The obtained extract was standardized based on the total anthocyanin content using the pH differential method (16 ). For this, two 1-g dried extract samples were dissolved in 10 mL of buffer solution (pH, 1) composed of 125 mL of KCl 0.2 M (Merck, Germany), 375 mL of HCl 0.2 M (Merck, Germany), and 10 mL of buffer solution with pH of 4.5 composed of 400 mL sodium acetate 1 M (Merck, Germany), 240 mL of HCl 1 M, and 360 mL of water. Both solutions were diluted 10 times with the same buffer and their absorbance was read at 510 nm using a spectrophotometer (PerkinElmer, USA). Total anthocyanin content was determined by the following equation:

In the above equation, 484.82 is the molecular mass of cyanidin-3-glucoside chloride, 24825 is molar absorptivity at 510 nm at pH = 1, and DF is the dilution factor that is 100.
Based on the above method, the anthocyanin content was 1.6 mg/g of the prepared powder.

Asgary S., Soltani R., Mirvakili S, & Sarrafzadegan N. (2016). Evaluation of the effect of Vaccinium arctostaphylos L. fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial. Research in Pharmaceutical Sciences, 11(4), 343-348.

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