Ovkate

HEK293T, OV90, HCT116, HT-29, ZR75-30, HCC1954 and BT549 were purchased from American Type Culture Collection (ATCC). OVKATE was purchased from JCRB Cell Bank. OV56, A2780 and IGROV1 were purchased from Sigma. COLO678 was purchased from Leibniz Institute DSMZ. Human ovarian PARP inhibitor and cisplatin-resistant cancer cell lines, PEO1/ABT-888 and OVCAR-1/Cisplatin and their parental cell lines, were gifts from Prof. Scott Kaufmann (Department of Oncology, Mayo Clinic). Cells were maintained under recommended culture conditions. PEO1/ABT-888 and OVCAR-1/Cisplatin were cultured in the media supplemented with 10 μM ABT-888 and 0.5 μg cisplatin, respectively. Cells were regularly tested for mycoplasma contamination.

OVCAR3, OVCAR5, SKOV3, and ES2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). OVCAR4 cells were gifted from Dr. Joanna Burdette (University of Illinois at Chicago). KURAMOCHI, OVKATE and OVSAHO were purchased from the JCRB cell bank (Osaka, Japan). SKOV3.ip1 cells were purchased from the cell bank at The University of Texas MD Anderson Cancer Center. All cells were authenticated by short tandem repeat (STR) analysis and passaged within 6 months of receipt. All cells were tested routinely for cell proliferation and BrdU incorporation as well as mycoplasma contamination. All cell lines exhibited similar morphology, characteristic growth rates, and remained negative for mycoplasma contamination throughout all experiments. OVCAR4, KURAMOCHI, OVKATE and OVSAHO cells were grown in RPMI 1640 with 10% FBS and penicillin/streptomycin, and other cells were cultured in DMEM with 10% FBS, 2mM glutamine and penicillin/streptomycin. All cell lines were grown in a humidified incubator at 37°C and constant 5% CO₂.

The human U2OS, HEK293T, OVCAR8, and OVCAR10 were purchased from ATCC. PEO1 and OV56 were bought from Sigma. OVKATE cells purchased from JCRB Cell Bank. Cells were immersed in McCoy's 5A, Dulbecco's Modified Eagle's Medium (DMEM), or RPMI1640, or added with 10% fetal bovine serum (FBS) in 5% CO2 at 37°C. pDEST51‐LRRK2 was purchased from Addgene (#25080). Full‐length LRRK2 was constructed into pLVX3 lentiviral vector and then lentivirus was packaged, and OVCAR10 cells were infected with the virus and selected with puromycin for 10 days to establish stable cell lines. pCMV‐Myc‐RAD51 was given by Dr. Junjie Chen (University of Texas MD Anderson Cancer Center). pCHMWS‐FLAG‐LRRK2 and mutations (K1906M,G2019S, and pS935/910) were gifts from Dr. Cookson (Molecular Genetics Section, Laboratory of Neurogenetics, National Institute on Aging). Anti‐RAD51 (GTX100469) antibody was purchased from GeneTex. Anti‐BRCA1 (SC‐6954), Antigreen fluorescent protein [GFP] (SC‐9996), Anti‐RPA2 (SC‐56770), and Anti‐Myc (sc‐40, mouse) antibodies were sold by Santa Cruz Biotechnology. Anti‐BRCA2 (pS3291,AB9986) was bought from sigma. Anti‐BRCA2 (OP95‐100UG) was purchased from Millipore Anti‐V5 (SV5‐Pk1) antibody, Anti‐LRRK2(pS910)(ab133450), and Anti‐LRRK2(pS935)(ab133449) were purchased from Abcam.