Catechin hydrate c1251

For modification of the motherwort tincture, a number of amino acids were used: lysine, aspartic and glutamic acids, leucine, alanine, phenylalanine, glycine, valine, histidine, arginine, cysteine, and methionine (a set of amino acids No. 2 big TU 6-09-3147-91, Chemical reagent kit).
Sucrose (Compri Sugar O, Witec industrial, Odessa, Ukraine) and ethanol (Biolik, Melitopol, Ukraine) were used to obtain the extracts.
The following reagents of the class “chemically pure” and “chemically pure for analysis” were used for TLC, HPLC, and spectrophotometric studies: glacial acetic acid (100063.2500, Merck, Darmstadt, Germany), ethyl acetate (Khimreserv, Kiev, Ukraine), chloroform (102444.1000, Merck), methanol (106007.2500, Merck); dimethylaminobenzaldehyde (156477 Sigma-Aldrich, St. Louis, MO, USA), hydrochloric acid (141020.1214, Merck), hydroxylamine (255580 Sigma-Aldrich, USA), and aluminum oxide (010091, Reakhim, Kharkiv, Ukraine).
Standard substances manufactured by Sigma-Aldrich, USA (chlorogenic acid C3878, caffeic acid C0625, rutin hydrate R5143, ellagic acid E2250, quercetin 3-d-galactoside 83388, rosmarinic acid R4033, (+)-catechin hydrate C1251, and 8-acetylharpagide SMB00118) were used for analysis.

Dried butterfly pea (Clitoria ternatea) flowers were purchased from a local market in Chiang Rai, Thailand. Sodium alginate was obtained from Union Science Co., Ltd. (Chaing Mai, Thailand). Agar was purchased from Krungthepchemi Co., Ltd. (Bangkok, Thailand). Catechin hydrate (C1251) and lysozyme from chicken egg white (62971) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Escherichia coli TISTR 527 and Staphylococcus aureus TISTR 746 were received from the Biological Laboratory (Mae Fah Luang University, Chiang Rai, Thailand). All other chemicals employed in this study were of analytical grade.