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2 protocols using dylight 405 labeled goat anti mouse igg

1

Antibody Panel for Cell Signaling

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The primary antibodies to Drp1, phospho-Drp1 (Ser616), p53, and cleaved-caspase 3 were purchased from Cell Signaling Technology (CST, MA, USA). Phospho-Drp1 (Ser637), ABCG2 (ATP-binding cassette sub-family G member 2), and Oct4 (octamer-binding transcription factor 4), ALDH1 (aldehyde dehydrogenase 1), and BAX (Bcl-2-associated X protein) antibodies were purchased from Ruiyingbio (Jiangsu, China). Mfn2 antibody was obtained from Abgent (NJ, USA). The antibody against β-actin was from Boster (Wuhan, China). The antibodies against COXIV, CD133, CD44, BMI1 (B lymphoma Mo-MLV insertion region 1 homolog), and Bcl-2 (B-cell lymphoma 2) were from Beyotime (Shanghai, China). LC3 antibody was purchased from Novus Biologicals (CO, USA). The peroxidase-conjugated affinipure secondary antibodies goat anti-rabbit IgG and anti-mouse IgG were purchased from Thermo. Alexa Fluor® 488-labeled goat anti-rabbit IgG and DyLight® 405-labeled goat anti-mouse IgG were obtained from Beyotime.
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2

Antibody Validation for Western Blotting

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The following antibodies were used in western blotting assays: anti-COX-2, anti-AKT, anti-ERK, anti-CDK2, anti-Ac-lysine, and anti-SIRT3 (Cell Signaling Technology, Danvers, MA, USA), anti-Drp1 and anti-p-Drp1Ser616 (Ruiying Biological, Jiangsu, China), anti-p38 MAPK, anti-PKCα, anti-PINK1, anti-GAPDH, anti-Bax, anti-Bcl-2, anti-cleaved-Caspase-3, anti-COXIV, anti-Flag, Alexa Fluor® 488-labeled goat anti-rabbit IgG, Alexa Fluor® 488-labeled goat anti-mouse IgG, and DyLight® 405-labeled goat anti-mouse IgG (Beyotime, Shanghai, China). The peroxidase-conjugated affinipure secondary antibodies goat anti-rabbit IgG and anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). cDDP, RSV, dimethyl sulfoxide (DMSO), and Mdivi-1 were purchased from Sigma Aldrich (St. Louis, MO, USA). Carboplatin (CBP) was purchased from Hansoh Pharmaceutical Co., Ltd. (Jiangsu, China), and oxaliplatin (L-OHP) was purchased from Selleck (Santa Clara, TX, USA).
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