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Fluorescent dye conjugated antibodies

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Fluorescent dye-conjugated antibodies are laboratory reagents used to label and detect specific target proteins or cells. They consist of an antibody molecule chemically coupled to a fluorescent dye. The dye emits light when excited by a specific wavelength, allowing for the visualization and quantification of the labeled target.

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Lab products found in correlation

P iκb, by Cell Signaling Technology (1 mentions) Docetaxel, by Enzo Life Sciences (1 mentions) P ikkα β, by Cell Signaling Technology (1 mentions) P stat3, by Abcam (1 mentions) Interleukin 6 (il 6), by GeneTex (1 mentions)

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Fluorescent-conjugated antibodies (3 citations)

2 protocols using fluorescent dye conjugated antibodies

1

Multiparameter Flow Cytometry Analysis

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Fluorescent dye-conjugated antibodies that are specific for CD3, CD4, CD8, PD-1, and PD-L1, respectively were obtained from BioLegend. Cells were stained with the antibodies either alone or in combinations and analyzed by flow cytometry.
Liu M.Y., Klement J.D., Langan C.J., van Riggelen J, & Liu K. (2021). Expression regulation and function of PD-1 and PD-L1 in T lymphoma cells. Cellular immunology, 366, 104397.
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2

Investigating Inflammatory Signaling Pathways

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Commercial antibodies against IL6, CXCL8, p65 (GeneTex International Corp, Hsinchu City, Taiwan), p105/p50, p100/52 (EMD Millipore, Billerica, MA), pIKKα/β, pIκB (Cell Signaling Technology, Danvers, MA), IκB (Santa Cruz, Dallas, Texas) and CD31, phosphorylated p65, IKKβ, IKKα and pSTAT3 (Abcam, Cambridge, MA) were used. Chemokine ELISA kits were from R&D systems (Minneapolis, MN). Docetaxel was from Enzo Life Sciences (Farmingdale, NY). Fluorescent dye-conjugated antibodies used in the flow cytometry analysis were all from Biolegend (San Diego, CA).
Tsai C.H., Tzeng S.F., Hsieh S.C., Yang Y.C., Hsiao Y.W., Tsai M.H, & Hsiao P.W. (2017). A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer. Scientific Reports, 7, 15624.
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