Treosulfan was kindly supplied by Medac GmbH (Wedel, Germany). Human liver cytosol (20 mg protein/1 mL preparation in phosphate buffer), pooled from 46 adult donors representing different genders and race, was purchased from Life Technologies. GSH and racemic DEB, used as a positive control for testing the liver fraction activity, were obtained from Sigma-Aldrich. Acetonitrile and methanol, liquid chromatography (LC) gradient grade purity, and formic acid for LC were from Merck. Other chemicals, all of analytical grade, were also obtained from commercial suppliers. Demineralized water at a conductivity of 0.1 μS/cm, prepared in a deionizer and filtered through a 0.45-μm cellulose membrane filter, was always used.
Treosulfan
Manufactured by Medac
Sourced in Germany
Treosulfan is a cytotoxic agent used as a conditioning agent in hematopoietic stem cell transplantation. It is a bifunctional alkylating agent that inhibits DNA synthesis and cell division.
Automatically generated - may contain errors
3 protocols using treosulfan
1
Treosulfan Metabolism in Human Liver
2
Tumor Growth Inhibition in Mice
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Four- to six-week-old athymic-nude and SCID/NOD mice (Harlan Laboratories, Udine, Italy) were housed under specific pathogen-free conditions. Details on Tumor growth experiments in mice pre-treated or not with the myeloablative agent treosulfan (Medac) [50 (link)], and schedule of hrIL-27 administration are provided in the Supplementary information .
3
Treosulfan Reference Material Preparation
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A certified reference material of treosulfan for preparation of an injection solution and for analytical purposes was supplied by medac GmbH (Wedel, Germany). Xylazine (Sedazin) was bought from Biowet Puławy (Puławy, Poland) and ketamine (Bioketan) was from Vetoquinol Biowet (Gorzów Wlkp., Poland). Analytical grade citric acid was purchased from Avantor (Gliwice, Poland).
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