96 well screening plates

Oligonucleotides were from Integrated DNA Technologies (Coralville, IA). All other chemicals were obtained from either Sigma Aldrich (St. Louis, MO) or Fisher Scientific (Pittsburgh, PA). DNA sequencing was performed by Functional Bioscience (Madison, WI). Radioactive isotopes, SPA beads, and 96-well screening plates were from PerkinElmer (Waltham, MA). Escherichia coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant), and P. aeruginosa hypersensitive strain (ATCC 35151) were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from the American Type Culture Collection (ATCC) (Manassas, VA). Penicillin-Streptomycin Solution was from Mediatech, Inc. (Centerville, IA). The synthetic compound library was from TimTec LLC (Newark, DE), and the natural compound library was from MicroSource Discovery Systems (Gaylordsville, CT).

All chemicals were obtained from Fisher Scientific (Pittsburg, PA). Radioactive isotopes, SPA beads and 96-well screening plates were from PerkinElmer (Waltham, MA). E. coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant) and P. aeruginosa hypersensitive (ATCC^® 35151^™) strains were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from American Type Culture Collection (ATCC) (Manassas, VA). The synthetic compound library was from TimTec LLC (Newark, DE) and the natural product library was from MicroSourceDiscovery Systems, Inc. (Gaylordsville, CT). Compounds were supplied as 10 mM stocks dissolved in dimethyl sulfoxide (DMSO), stored at −20 °C and thawed immediately before analysis. The compounds have an average purity of 95%, and the minimum purity is at least 90%.

All chemicals were obtained from either Sigma Aldrich (St. Louis, MO) or Fisher Scientific (Pittsburgh, PA). Radioactive isotopes, SPA beads, and 96-well screening plates were from PerkinElmer (Waltham, MA). Escherichia coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant), and P. aeruginosa hypersensitive (ATCC 35151) strains were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from the American Type Culture Collection (ATCC) (Manassas, VA). Penicillin-streptomycin solution was from Mediatech, Inc. (Centerville, IA). The synthetic Anti-Infectives Library was from TimTec LLC (Newark, DE) and included 890 low-molecular-weight, druglike molecules with scaffolds found in antiseptic agents with antibacterial, antifungoid, and antimicrobial activities. Compounds were supplied as 10 mM stocks dissolved in DMSO, stored at −20 °C, and thawed immediately before analysis. The compounds have an average purity of 95%, and the minimum purity is at least 90%.