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Iba1 aif 1

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Iba1/AIF-1 is a protein that is involved in the activation and regulation of macrophages and microglia. It is commonly used as a marker for these cell types in research applications.

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Lab products found in correlation

Neuronal nuclei (neun), by Merck Group (2 mentions) Mab4234, by R&D Systems (2 mentions) Antigen down elisa, by R&D Systems (2 mentions) Glial fibrillary acidic protein (gfap), by BioLegend (2 mentions)

2 protocols using iba1 aif 1

1

Validated Antibody for GCPII Detection

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We used the mouse PSMA/FOLH1/NAALADase I/GCPII antibody raised against Lys44-Ala750 which contains the extracellular domain of GCPII (MAB4234; R&D Systems; 1:50). The antibody recognizes a very specific band for GCPII by western blotting with no other non-specific bands. The recognition of PSMA/FOLH1/GCPII was confirmed using antigen-down ELISA (R&D Systems). In direct ELISA’s, the antibody shows less than 10% cross-reactivity with rhNAALADase-like 2 and no cross-reactivity with rhNAALADase-like 1, rhNAALADase-like 3, rmNAALADase I, or rmNAALAase-like 2. The multiple label immunofluorescence used the following additional antibodies: GFAP (1:500, BioLegend, Cat# 829401), NeuN (1:300, EMD Millipore, Cat# ABN78), and Iba1/AIF-1 (1:300, Cell Signaling Tech, Cat# 17198 T).
Yang S., Datta D., Elizabeth W.o.o., Duque A., Morozov Y.M., Arellano J., Slusher B.S., Wang M, & Arnsten A.F. (2022). Inhibition of glutamate-carboxypeptidase-II in dorsolateral prefrontal cortex: potential therapeutic target for neuroinflammatory cognitive disorders. Molecular Psychiatry, 27(10), 4252-4263.
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2

Characterization of GCPII Antibody

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We used the mouse PSMA/FOLH1/NAALADase I/GCPII antibody raised against Lys44-Ala750 which contains the extracellular domain of GCPII (MAB4234; R&D Systems; 1:50). The antibody recognizes a very specific band for GCPII by western blotting with no other non-specific bands. The recognition of PSMA/FOLH1/GCPII was confirmed using antigen-down ELISA (R&D Systems). In direct ELISA’s, the antibody shows less than 10% cross-reactivity with rhNAALADase-like 2 and no cross-reactivity with rhNAALADase-like 1, rhNAALADase-like 3, rmNAALADase I, or rmNAALAase-like 2. The multiple label immunofluorescence used the following additional antibodies: GFAP (1:500, BioLegend, Cat# 829401), NeuN (1:300, EMD Millipore, Cat# ABN78), and Iba1/AIF-1 (1:300, Cell Signaling Tech, Cat# 17198 T).
Yang S., Datta D., Elizabeth W.o.o., Duque A., Morozov Y.M., Arellano J., Slusher B.S., Wang M, & Arnsten A.F. (2022). Inhibition of glutamate-carboxypeptidase-II in dorsolateral prefrontal cortex: potential therapeutic target for neuroinflammatory cognitive disorders. Molecular Psychiatry, 27(10), 4252-4263.
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