15n5 8 ohdg

Manufactured by Cambridge Isotopes

Sourced in United States

15N5-8-OHdG is a stable isotope-labeled compound used as an internal standard for the quantification of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, in various biological samples. It provides an accurate and precise measurement of 8-OHdG levels when used in conjunction with analytical techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

[15N5]8-Hydroxy-2′-deoxyguanosine ([15N5]8-OHdG) (2 citations)

5 protocols using 15n5 8 ohdg

Quantification of Oxidative Stress Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

8-OHdG (≥98%) (2-amino-9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,7-dihydropurine-6,8-dione) in a solid form was obtained from Merck (Buchs, St. Gallen, Switzerland). [¹⁵N5]-8-OHdG as internal standard (IS) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). 8-Isoprostane ((5Z,8β,9α,11α,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid) in a solution ≥ 95%, and its deuterated IS 8-Isoprostane-d₄ ((5Z,8β,9α,11α,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic-3,3,4,4-d4 acid), were obtained from Cayman Chemical (Ann Arbor, MI, USA). HPLC grade methanol (≥99.9%) was obtained from Merck (Buchs, Switzerland). LC-MS grade solvents; methanol (≥99.95%) and acetonitrile (≥99.9%) were obtained from Carlo Erba Reagents (Chaussée du Vexin, Val de Reuil, France). Butylated hydroxytoluene (BHT) ≥ 99% was obtained from Sigma-Aldrich Produktions GmbH (Steinheim Germany). LC–MS grade acetic acid was obtained from Honeywell (Seelze, Germany). Ultrapure water was produced in our laboratory with a Milli-Q Advantage water purification system (18.2 MΩ.cm at 25 °C, <3 ppb total organic carbon).

Hemmendinger M., Sauvain J.J., Hopf N.B., Suárez G, & Guseva Canu I. (2022). Challenges in Quantifying 8-OHdG and 8-Isoprostane in Exhaled Breath Condensate. Antioxidants, 11(5), 830.

+ Open protocol

+ Expand

Quantifying Oxidative Stress Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

o,o’-dityrosine (diY) (>99%) was purchased from Toronto Research Chemicals (Toronto, Ontario, Canada). 8-hydroxy-2’-deoxyguanosine (8-OHdG) (≥98%), malondialdehyde tetrabutyl-ammonium salt, acetic acid, n-hexane, LC-MS grade methanol and water, acetonitrile, ethyl acetate, and synthetic urine were purchased from Sigma Aldrich (St. Louis, MO, USA). 8iso-prostaglandin F_2α (8-PGF_2α) (>99%), 15(R)-prostaglandin F_2α (15-PGF_2α) (>98%), 8-iso15(R)-prostaglandin F_2α (8,15-PGF_2α) (>98%), 11β-prostaglandin F_2α (11-PGF_2α) (>98%), and the internal standards (IS) for F₂-isoprostanes, D₄-8-iso-prostaglandin F_2α (D₄-8-PGF_2α) (>99%), were purchased from Cayman Chemicals (Ann Arbor, MI, USA). 2,4–1,1,3,3-, tetraethoxypropane-1,3-d₂ (>98%) (IS for MDA) was purchased from C/D/N isotopes (PointClair, Quebec, Canada). ¹³C₁₂-o,o’-dityrosine (IS for diY) and ¹⁵N₅-8-OHdG (IS for 8-OHdG) were purchased from Cambridge Isotope Laboratories (Andover, MA, USA). Derivatization reagent 2,4-dinitrophenylhydrazine (DNPH) was purchased from Spectrum (New Brunswick, NJ, USA).

Martinez-Moral M.P, & Kannan K. (2018). How stable is oxidative stress level? An observational study of intra- and inter-individual variability in urinary oxidative stress biomarkers of DNA, proteins, and lipids in healthy individuals. Environment international, 123, 382-389.

+ Open protocol

+ Expand

Oxidative Stress Biomarkers Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For this study, 8-OHdG was purchased from Sigma-Aldrich (St. Louis, MO, USA); 15N5-8-OHdG was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA), and 8-iso-PGF2α and 8-iso-PGF2α-d4 were from Cayman Chemicals Co. (Ann Arbor, MI, USA). Amicon^® ultracentrifugal filters (Ultracel^®-30 K) were purchased from Merck Millipore, Ltd. (Cork, Ireland), and Sep-Pak^® C18 solid-phase extraction (SPE) cartridges (3 cc, 500 mg) were purchased from Waters (Milford, MA, USA). Purified water was obtained from the Milli-Q^® Integral system (Merck Millipore Ltd., Cork, Ireland). All other chromatography-grade chemicals were obtained from Sigma-Aldrich.

Di Minno A., Aveta A., Gelzo M., Tripodi L., Pandolfo S.D., Crocetto F., Imbimbo C, & Castaldo G. (2022). 8-Hydroxy-2-Deoxyguanosine and 8-Iso-Prostaglandin F2α: Putative Biomarkers to assess Oxidative Stress Damage Following Robot-Assisted Radical Prostatectomy (RARP). Journal of Clinical Medicine, 11(20), 6102.

+ Open protocol

+ Expand

Quantification of Oxidative Stress Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

HNE-MA (1mg in 100 μl ethanol), HNE-MA-d₃ (100 μg in 100 μl ethanol), 8-isoPGF_2α, and 8-isoPGF_2α-d₄ were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Maleic anhydride, 8-OHdG, and 7.5M of ammonium acetate solution (NH₄Ac_(aq)), 0.1 N sodium hydroxide (NaOH) standard solutions, and were purchased from Sigma–Aldrich (St. Louis, MO, USA). The internal standard, ¹⁵N₅-8-OHdG (99% purity), was acquired from Cambridge Isotope Laboratories (Andover, MA, USA). Unlabeled 8-NO₂Gua and its internal standard, 8-NO₂Gua-4, 8-¹³C₂-7-¹⁵N, were obtained from Santa Cruz Biotech (Santa Cruz, CA, USA). HPLC-grade methanol (MeOH) was procured from MACRON Chemicals (Center Valley, PA). For all subsequent steps, Milli-Q water was produced by a Millipore Elix 10 RO system and a Millipore Synergy UV system (Millipore SAS, Molsheim, France).

Wu C., Chen H.C., Chen S.T., Chiang S.Y, & Wu K.Y. (2017). Elevation in and persistence of multiple urinary biomarkers indicative of oxidative DNA stress and inflammation: Toxicological implications of maleic acid consumption using a rat model. PLoS ONE, 12(10), e0183675.

+ Open protocol

+ Expand

Quantification of Oxidative Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

8-OHdG and HPLC-grade methanol were obtained from Sigma-Aldrich (St. Louis, MO, USA), HNE-MA was obtained from Zzstandard (Shanghai Zzbio Co. Ltd., Shanghai, China), the internal standards 15 N5-8-OHdG (25 μg/ml in water, 98% purity) and HME-NA-d3 (>98% purity) were obtained from Cambridge Isotope Laboratories (Andover, MA, USA), and 8-isoPGF_2α and its internal standard 8-isoPGF_2α-d4 were obtained from Cayman Chemicals (Ann Arbor, MI, USA). Purified water was obtained using a Milli-Q system (Millipore, Bedford, MA, USA). Ammonium acetate was purchased from Sinopharm Chemicals (Shanghai, China).

Cai X., Ning C., Fan L., Li Y., Wang L., He H., Dong T., Cai Y., Zhang M., Lu Z., Chen C., Shi K., Ye T., Zhong R., Tian J., Li H., Li H., Zhu Y, & Miao X. (2023). Triclosan is associated with breast cancer via oxidative stress and relative telomere length. Frontiers in Public Health, 11, 1163965.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!