Imaging was performed using tapping mode on a Multimode VIII atomic force microscope (AFM) with an E-scanner (Bruker). Imaging was performed in TAE/Mg2+ buffer solution with DNP-S oxide-sharpened silicon nitride cantilevers and SNL sharp nitride levers (Bruker Probes) using resonance frequencies between 7–9 kHz of the narrow 100 μm, 0.38 N/m force constant cantilever. After self-assembly of the origami structure ≈20 μl of TAE/Mg2+ buffer solution was deposited onto a freshly cleaved mica surface (Ted Pella) glued to a metal puck (Ted Pella). After 30 s the mica surface was dried using a gentle stream of N2 and 5 μl of the origami solution was deposited onto the mica surface. After another 30 s, 30 μl of additional buffer solution was added to the sample. Imaging parameters were optimized for best image quality while maintaining the highest possible setpoint to minimize damage to the samples. Images were post-processed by subtracting a 1st order polynomial from each scan line. Drive amplitudes were approximately 0.11 V, integral gains ≈2, and proportional gains ≈4.
Metal puck
Manufactured by Ted Pella
The metal puck is a general-purpose laboratory equipment piece. It is a flat, circular metal disk that can be used for various purposes in a laboratory setting. The core function of the metal puck is to provide a stable and durable surface for holding or supporting samples, specimens, or other materials during experiments or analysis.
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Lab products found in correlation
2 protocols using metal puck
1
Atomic Force Microscopy of DNA Origami
2
Atomic Force Microscopy of DNA Origami
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Imaging was performed using tapping mode on a Multimode VIII atomic force microscope (AFM) with an E-scanner (Bruker). Imaging was performed in TAE/Mg2+ buffer solution with DNP-S oxide-sharpened silicon nitride cantilevers and SNL sharp nitride levers (Bruker Probes) using resonance frequencies between 7–9 kHz of the narrow 100 μm, 0.38 N/m force constant cantilever. After self-assembly of the origami structure ≈20 μl of TAE/Mg2+ buffer solution was deposited onto a freshly cleaved mica surface (Ted Pella) glued to a metal puck (Ted Pella). After 30 s the mica surface was dried using a gentle stream of N2 and 5 μl of the origami solution was deposited onto the mica surface. After another 30 s, 30 μl of additional buffer solution was added to the sample. Imaging parameters were optimized for best image quality while maintaining the highest possible setpoint to minimize damage to the samples. Images were post-processed by subtracting a 1st order polynomial from each scan line. Drive amplitudes were approximately 0.11 V, integral gains ≈2, and proportional gains ≈4.
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