Rabbit anti poly adp ribose polymerase parp

To determine nuclear and/or cytoplasmic Nrf2 levels in the cultured cells, the fractionated proteins were electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore Corp., Billerica, MA, USA). Primary antibodies used in this study were as follows: rabbit anti-Nrf2 (abcam, Cambridge, UK; Cat # ab62352), goat anti-Lamin B (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; Cat # SC-6216), rabbit anti-Bcl-2-associated X protein (Bax; abcam; Cat # ab32503), rabbit anti-B-cell lymphoma 2 (Bcl-2; abcam; Cat # ab32124), rabbit anti-cytochrome C (abcam; Cat # ab133504), rabbit anti-poly (ADP-ribose) polymerase (PARP; Cell Signaling Technology, Inc., Danvers, MA, USA; Cat # 9542S), and mouse anti-β-actin (Santa Cruz Biotechnology; Cat # SC-47778). Secondary antibodies used were anti-mouse, anti-rabbit or anti-goat IgG, conjugated to horseradish peroxidase (Santa Cruz Biotechnology). Protein bands were visualized using the SuperSignal^® West Pico Chemiluminescent Substrate kit (Thermo Fisher Scientific) and imaged using ImageQuant LAS 4000 mini (GE Healthcare Life Sciences, Little Chalfont, UK). Densitometric analysis was performed using Image Studio^TM Lite software (LI-COR Corp., Lincoln, NE, USA).

AA was isolated and identified as previously described (Figure 1A) [25 (link)]. AA was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution, which was further diluted in media to the desired working concentration. DMSO was used at ≤0.1% concentration, which was non-toxic to all cells tested.
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Invitrogen (Carlsbad, CA, USA). Hoechst 33342 and compound C (CC, Dorsomorphin) were obtained from Sigma Chemical (St. Louis, MO, USA). Annexin-V-FITC and propidium iodide (PI) were purchased from Immuno Tools (Friesoythe, Germany). MG132 was obtained from Merck Millipore (Billerica, MA, USA). Primary and secondary antibodies included: rabbit anti-Bcl-2, rabbit anti-poly-ADP-ribose polymerase (PARP), rabbit anti-caspase 3, rabbit anti-AMPK, rabbit anti-mTOR, rabbit anti-Bax, mouse anti-tubulin, anti-rabbit and anti-mouse IgG HRP-linked (Cell Signaling Technology, Beverly, MA, USA). Mouse anti-GADPH was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).