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Novolin r insulin

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Novolin-R is a short-acting insulin product manufactured by Novo Nordisk. It is used to manage blood sugar levels in individuals with diabetes. The core function of Novolin-R is to provide a fast-acting insulin to help regulate glucose in the body.

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Lab products found in correlation

Trueresult glucometer, by Nipro (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Nonessential amino acid, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Pen strep, by Cell Biolabs (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Cell Biolabs (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Plat e cells, by Cell Biolabs (1 mentions) Minimum essential medium (mem), by American Type Culture Collection (1 mentions) Penicillin, by American Type Culture Collection (1 mentions) Streptomycin, by American Type Culture Collection (1 mentions)

Spelling variants of this product

Insulin (160 citations) Novolin R (129 citations) Novolin (69 citations) Insulin (Novolin) (3 citations) Novolin R regular insulin (3 citations)

4 protocols using novolin r insulin

1

Intraperitoneal Glucose Tolerance Test

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Mice from HFD and LFD feeding studies were fasted for 6 h and weighed prior to an intra-peritoneal administration of dextrose (2 g/kg) or Novolin-R insulin (2 U/kg, Novo-Nordisk, Princeton NJ) in sterile saline. Blood glucose was sampled from a tail clip at zero time (i.e. prior to glucose or insulin administration) and at intervals thereafter and measured using a TRUEresult glucometer (Nipro Diagnostics, Fort Lauderdale, FL).
Goodson M.L., Knotts T.A., Campbell E.L., Snyder C.A., Young B.M, & Privalsky M.L. (2020). Specific ablation of the NCoR corepressor δ splice variant reveals alternative RNA splicing as a key regulator of hepatic metabolism. PLoS ONE, 15(10), e0241238.
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2

Glucose and Insulin Tolerance Testing in Mice

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Glucose-tolerance tests were performed in 8-week-old mice fed with Cordyceps supplemented diets for 4-weeks after an overnight fast. Glucose was injected (intraperitoneal injection of a 20% solution, 10 mL/kg body weight) and blood glucose concentrations in blood were measured after 0, 15, 30, 60 and 120 min with a glucometer (Bayer Contour, Bayer, Germany). Insulin-tolerance tests were performed in 8-week-old mice fed ad libitum. After determination of basal blood glucose concentrations, each mouse received an intraperitoneal injection of insulin (0.75 IU per kg body weight; Novolin R Insulin; Novo Nordisk) and glucose concentrations in blood were measured after 15, 30, 45 and 60 min.
Li Y., Talbot C.L., Chandravanshi B., Ksiazek A., Sood A., Chowdhury K.H., Maschek J.A., Cox J., Babu A.K., Paz H.A., Babu P.V., Meyerholz D.K., Wankhade U.D., Holland W., Shyong Tai E., Summers S.A, & Chaurasia B. (2022). Cordyceps inhibits ceramide biosynthesis and improves insulin resistance and hepatic steatosis. Scientific Reports, 12, 7273.
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3

Culturing 3T3, 3T3-Neu, and NT2 Cell Lines

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The 3T3 cell line was obtained from ATCC and cultured in DMEM (Gibco) supplemented with 10% FBS (Gemini) and 1% penicillin/streptomycin (Pen/Strep; Invitrogen). 3T3-Neu and NT2 tumors have been described (Reilly et al., 2000 (link)). 3T3-Neu cultures were made as described and cultured with the addition of 0.3 µM methotrexate. NT2 cells (passage 6) were cultured in RPMI-1640 (Gibco) supplemented with 20% FBS, 2 mM L-glutamine (Invitrogen), 12 mM Hepes (Invitrogen), 0.1 mM nonessential amino acid (Invitrogen), 1 mM sodium pyruvate (Invitrogen), 1% Pen/Strep, 50 µM 2-mercaptoethanol (Invitrogen), and 0.2 U/ml Novolin R-insulin (Novo Nordisk). The virus encoding the LH28z CAR was cultured in PLAT-E cells from Cell Biolabs in DMEM supplemented with 10% FBS and 1% Pen/Strep. All cells used for the generation of the CAR constructs were used within three passages in vitro, and 0.05% trypsin (Invitrogen) was used for digestion between passages.
Xu N., Palmer D.C., Robeson A.C., Shou P., Bommiasamy H., Laurie S.J., Willis C., Dotti G., Vincent B.G., Restifo N.P, & Serody J.S. (2020). STING agonist promotes CAR T cell trafficking and persistence in breast cancer. The Journal of Experimental Medicine, 218(2), e20200844.
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4

Cell Culture and Transfection Protocols

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MCF-7 breast adenocarcinoma (HTB-185), and D283 Med medulloblastoma (HTB-22) cell lines were procured from ATCC (Manassas, VA). Both cell lines were cultured per ATCC instructions, for the former in DMEM (Mediatech, Manassas, VA) supplemented with fetal bovine serum (10%), penicillin (100U mL−1), streptomycin (100 µgmL−1), (Mediatech, Manassas, VA), and Novolin R insulin (44 U L−1) (Novo Nordisk, Plainsboro, NJ), and for the latter in MEM (Mediatech, Manassas, VA) supplemented with fetal bovine serum (10%), penicillin (100U mL−1), streptomycin (100 µgmL−1), (Mediatech, Manassas, VA), and β-mercaptoethanol (10µM).
HEK 293T cells (a gift from Dr. Craig Walsh, University of California, Irvine) were cultured per ATCC instructions in MEM supplemented with fetal bovine serum (10%), penicillin (100U mL−1), streptomycin (100 µgmL−1), sodium pyruvate (1mM), and non-essential amino acid mixture (1X). HEK 293Ts were transiently transfected with pBLAST2-hFLK1 (InvivoGen, San Diego, CA) using BioT transfection reagent (Bioland Scientific, Paramount, CA) using conditions established by the manufacturer. HEK293T cells transiently transfected with pBLAST2-hFLK1 were utilized for flow cytometry or micropallet array studies 48 hours post transfection.
Westerhof T.M., Li G.P., Bachman M, & Nelson E.L. (2016). Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype. Advanced healthcare materials, 5(7), 767-771.
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