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P s235 236 s6

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The P-S235/236-S6 is an antibody that specifically recognizes the phosphorylated form of the S6 ribosomal protein at serine residues 235 and 236. This antibody can be used to detect the activation of the mTOR signaling pathway, which regulates protein synthesis and cell growth.

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Lab products found in correlation

Pt37 46 4ebp1, by Cell Signaling Technology (2 mentions) Pt389 s6k1, by Cell Signaling Technology (2 mentions) 4e bp1, by Cell Signaling Technology (2 mentions) Bcl 2, by Agilent Technologies (1 mentions) 7 aad, by BioLegend (1 mentions) Panαβtcr ip26, by Thermo Fisher Scientific (1 mentions) Panγδtcr 5a6 e9, by Thermo Fisher Scientific (1 mentions) Vδ1 rea173, by Miltenyi Biotec (1 mentions) P s380 pten, by Cell Signaling Technology (1 mentions) Tg 003, by Focus Biomolecules (1 mentions) Harmine, by Focus Biomolecules (1 mentions) Ps9 gsk3β, by Cell Signaling Technology (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Pt308 akt, by Cell Signaling Technology (1 mentions) Rheb activation kit, by Abcam (1 mentions) Vps34, by Cell Signaling Technology (1 mentions) Pf 4708671, by Selleck Chemicals (1 mentions) Cos 7 cells, by American Type Culture Collection (1 mentions) Nih3t3 cells, by American Type Culture Collection (1 mentions)

Spelling variants of this product

Anti-p-S6 (S235/236) (9 citations) Anti-p-S6(S235/236) (D57.2.2E) (3 citations) Rabbit antibodies against p-S6 (S235/236) (#4857) (2 citations) Phosphorylated rpS6 (S6 p-S235/236) (2 citations)

3 protocols using p s235 236 s6

1

Comprehensive Immune Cell Profiling

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Anti-human monoclonal antibodies were used against: CD3 (UCHT1), CD27 (LG.7F9), CD4 (RPA-T4), CD7 (4H9) and panαβTCR (IP26) from eBioscience; CD28 (CD28.2), CD8 (SK1), CD45 (HI30), Vδ2 (B6), CD3 (OKT3), CD45RA (HI100), Annexin-V and 7-AAD from Biolegend; panγδTCR (5A6.E9) from ThermoFisher; Vδ1 (REA173) from Miltenyi Biotech; p-S129-AKT, AKT, p-S9-GSK3β, GSK3β, p-S380-PTEN, PTEN, p-S235/236-S6 and S6 from Cell Signaling; Calnexin and GAPDH from Sicgen; 7-AAD from Invitrogen and Bcl-2 from Dako. Recombinant human sCD27 ligand and recombinant human IL-2 were purchased from Peprotech; CX-4945 (Silmitasertib) from Adooq Bioscience and Biorbyt; TBB from Sigma; TG-003 and Harmine from Focus Biomolecules.
Ribeiro S.T., Tesio M., Ribot J.C., Macintyre E., Barata J.T, & Silva-Santos B. (2016). Casein kinase 2 controls the survival of normal thymic and leukemic γδ T cells via promotion of AKT signaling. Leukemia, 31(7), 1603-1610.
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2

Western Blot Analysis of Signaling Pathways

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Cells were rinsed once with ice-cold PBS and lysed in ice-cold lysis buffer (50 mM HEPES [pH 7.4], 40mM NaCl, 2mM EDTA, 1.5mM sodium orthovanadate, 50mM NaF, 10mM pyrophosphate, 10mM glycerophosphate, and 1% Triton X-100, and one tablet of EDTA-free complete protease inhibitors [Roche] per 25ml). Cell lysates were cleared by centrifugation at 13,000rpm for 10 min. Proteins extracts were denatured by the addition of sample buffer, boiled for 5min, resolved by SDS-PAGE, and analyzed by immunoblotting. Western blot analyses were performed according to standard procedures. Antibodies from Cell Signaling were used for detection of P-T389-S6K1 (#9234), S6K1 (#2708), P-S235/236-S6 (#2211), S6 (#2217), P-T37/46-4EBP1 (#2855), 4EBP1 (#9644), P-T308-AKT (#2965), P-S743-AKT (#4060), AKT (#4691). For detection of β-actin we used an antibody from Sigma (#A5441).
Ortega-Molina A., Deleyto-Seldas N., Carreras J., Sanz A., Lebrero-Fernández C., Menéndez C., Vandenberg A., Fernández-Ruiz B., Marín-Arraiza L., de la Calle Arregui C., Belén Plata-Gómez A., Caleiras E., de Martino A., Martínez-Martín N., Troulé K., Piñeiro-Yáñez E., Nakamura N., Araf S., Victora G.D., Okosun J., Fitzgibbon J, & Efeyan A. (2019). Oncogenic Rag GTPase signaling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR. Nature metabolism, 1(8), 775-789.
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3

Investigating mTOR Signaling Pathway

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NIH3T3 cells and COS-7 cells were purchased from American Type Culture Collection (ATCC) and used within six months after cells were ordered. These cell lines were not authenticated in our laboratory. Rapamycin, 5-Bromo-2′-deoxyuridine (BrdU) and cycloheximide were obtained from Sigma. PF-4708671 was purchased from Selleckchem. MG132 was purchased from CalBiochem. Antibodies directed against Myc-tag (9E10), Flag-tag, and HA-tag were obtained from Covance. Antibodies directed against VPS34 (cat#4263), VPS15 (cat#14580), Beclin 1 (cat#3495), pT389-S6K1 (cat#9234), S6K1 (cat#2708), pT37/46-4EBP1 (cat#2855), 4EBP1 (cat#9644), pS235/236-S6 (cat#4858), S6 (cat#2217), pErk1/2 (cat#4370), Erk1/2 (cat#4695), pS473-Akt(cat#4060), Akt (cat#4685), RheB (cat#13879), TSC1 (cat#6935), TSC2 (cat#4308), BrdU (cat#5292), and Cyclin E (cat#4129) were purchased from Cell Signaling Technology. Fluorescently tagged antibodies were obtained from Invitrogen. The class III PI3K lipid kinase ELISA kit was purchased from Echelon Biosciences. The RheB activation kit was purchased from Abcam. 4-6 weeks old SCID mice were ordered from National Cancer Institute (NCI), NIH (Bethesda, MD).
Mohan N., Shen Y., Dokmanovic M., Endo Y., Hirsch D.S, & Wu W.J. (2016). VPS34 regulates TSC1/TSC2 heterodimer to mediate RheB and mTORC1/S6K1 activation and cellular transformation. Oncotarget, 7(32), 52239-52254.
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