Multicell

Manufactured by Mediso

Sourced in Hungary

The MultiCell is a versatile lab equipment designed for cell culture applications. It provides a controlled environment for growing and maintaining cells. The core function of the MultiCell is to facilitate the culture and propagation of various cell types for research and experimentation purposes.

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Lab products found in correlation

Tera tomo, by Mediso (2 mentions) 67ga citrate, by PerkinElmer (1 mentions) Nanoscan pet mri system, by Mediso (1 mentions) Amira 5, by Thermo Fisher Scientific (1 mentions) Nanospect ct, by Bioscan (1 mentions)

Spelling variants of this product

MultiCell Imaging Chamber (4 citations)

6 protocols using multicell

In Vivo Multi-Modal Molecular Imaging

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For SPECT, 4.1–5.4 MBq of ⁶⁷Ga-citrate (CIS Bio, France) was injected iv. in 0.2 mL 24 h before imaging A 45-min SPECT scan was then performed using a NanoSPECT/CT Silver Upgrade small animal SPECT system (Mediso Ltd, Hungary) using both the 93 keV and 184 keV photopeaks in a 20–20% energy window, with 60 acquisition views and a Monte–Carlo model based three-dimensional SPECT reconstruction (Tera-Tomo, Mediso Ltd, Hungary) resulting in an image volume of 0.6 mm voxels. The same animal in the same animal bed allowing constant position was then immediately subjected to a whole-body three-dimensional MR imaging sequence in the nanoScan 1T MRI subcomponent. After these acquisitions, tissues were examined with ex vivo CLI, histology and immunohistochemistry following the in vivo imaging. All live animal image acquisitions were performed under 2% isoflurane anesthesia in medical oxygen in a special cross-compatible animal holder bed (MultiCell, Mediso, Hungary) which kept the animals immobilised during cross-modality and cross-device scans.

Ritter Z., Zámbó K., Balogh P., Szöllősi D., Jia X., Balázs Á., Taba G., Dezső D., Horváth I., Alizadeh H., Tuch D., Vyas K., Hegedűs N., Kovács T., Szigeti K., Máthé D, & Schmidt E. (2021). In situ lymphoma imaging in a spontaneous mouse model using the Cerenkov Luminescence of F-18 and Ga-67 isotopes. Scientific Reports, 11, 24002.

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Multimodal Imaging of Mice with FDG-PET/MRI

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Mouse PET/MRI measurements were performed in a MultiCell™ heating, positioning and monitoring multi-animal bed on an nanoScan 1T integrated imaging systems (both Mediso Ltd., Budapest, Hungary). Ninety minutes after FDG injection, a 15 min static PET data acquisition was obtained in an energy window of 350–750 keV, immediately followed by a three-dimensional T1-weighted gradient echo sequence, with 300 micron voxel size, 6 excitations, 12.1 ms/2.9 ms Transmit/Receive times, and 15 degrees of flip angle. Quantitative radioactivity PET data were reconstructed using a Monte-Carlo based iterative algorithm (Tera-Tomo™, Mediso Ltd., Budapest, Hungary) using the MRI as anatomical and attenuation priors, with 0.3 mm voxel size for a whole-body PET/MRI image with a resolution of 1 mm.

Ritter Z., Zámbó K., Jia X., Szöllősi D., Dezső D., Alizadeh H., Horváth I., Hegedűs N., Tuch D., Vyas K., Balogh P., Máthé D, & Schmidt E. (2021). Intraperitoneal Glucose Transport to Micrometastasis: A Multimodal In Vivo Imaging Investigation in a Mouse Lymphoma Model. International Journal of Molecular Sciences, 22(9), 4431.

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Whole-Body PET Imaging in Mice, Piglets and Humans

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The animals were positioned prone in a special mouse imaging chamber (MultiCell, MEDISO Budapest, Hungary), with the head fixed to a mouth piece for the anesthetic gas supply. The PET data was collected in list-mode by a continuous whole-body (WB) scan during the entire investigation using the whole FOV at one bed position (BP). Subsequently, the list-mode-data was rebinned into sinograms of time frames (3 × 5 min, 1 × 10 min, 6 × 15 min) up to 240 min. p.i. (Fig. 1). Following the PET scan, a T1-weighted WB gradient echo sequence (TR = 20 ms; TE = 3.2 ms) was performed for anatomical orientation and segmentation of a μ-map (soft tissue and air) for AC.Fig. 1

Dynamic PET image series (MIP) of mice (a), piglets (b), and humans (c). Each series shows the tracer accumulation in whole brain and particularly in the striatum followed by a washout through the hepatobiliary and the renal system. The animals did not void during the course of imaging. The healthy volunteers were asked to void as indicated in the timeline of the human investigational protocol

Kranz M., Sattler B., Tiepolt S., Wilke S., Deuther-Conrad W., Donat C.K., Fischer S., Patt M., Schildan A., Patt J., Smits R., Hoepping A., Steinbach J., Sabri O, & Brust P. (2016). Radiation dosimetry of the α4β2 nicotinic receptor ligand (+)-[18F]flubatine, comparing preclinical PET/MRI and PET/CT to first-in-human PET/CT results. EJNMMI Physics, 3, 25.

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Quantitative PET/MRI Imaging of Tumors

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PET/MRI fused imaging was performed using the nanoScan PET/MRI system (1T, Mediso, Hungary). Mice were fasted for 8 h before imaging, maintained at a constant body temperature, and injected intravenously via the tail vein with 6.5 ± 1.0 MBq in 0.2 mL of FDG. Mice were kept under anaesthesia (1.5% isoflurane in 100% O₂ gas). The T1-weighted with Gradient-echo (GRE) 3D sequence (TR = 25 ms, TEeff = 3.4, FOV = 64 mm, matrix = 128 × 128) was acquired during the FDG uptake period. Static PET images were acquired for 10 min in a 1–5 coincident in a single field of view in the MRI range. Body temperature was maintained with a heating pad on the animal bed (Multicell, Mediso, Hungary) and a pressure sensitive pad was used for respiratory triggering. PET images were reconstructed by Tera-Tomo 3D in full detector mode with all the corrections on, high regularisation, and eight iterations. Three-dimensional volume of interest (VOI) analysis of the reconstructed images was performed using the InterView Fusion software package (Mediso, Hungary) and applying standard uptake value (SUV) analysis. The VOI was fixed in a sphere of 2 mm diameter, which was drawn for the tumour and muscle sites. The SUV of each VOI site was calculated using the following formula SUV mean = (tumour radioactivity in the tumour VOI with the unit of Bq/cc × body weight) divided by injected radioactivity^{59 (link)}.

Jun E., Kim S.C., Lee C.M., Oh J., Lee S, & Shim I.K. (2017). Synergistic effect of a drug loaded electrospun patch and systemic chemotherapy in pancreatic cancer xenograft. Scientific Reports, 7, 12381.

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Multimodal Imaging of MSC Therapy in IC/BPS

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Ten HCl-based IC/BPS rats were randomly divided into two groups and injected with 1 × 10⁶ M-MSC (n = 5) or PBS vehicle (n = 5). At 6, 9, and 12 months after injection, μ-MRI/PET imaging was performed using the nanoScanPET/MRI imaging system (1 T, MEDISO, Budapest Hungary). Rats were fasted for 8 hours prior to imaging. Rats were administered 19.7 ± 1.1 MBq in 0.2 mL of 2-[¹⁸F]-FDG via the tail vein while the rat was under anesthesia (2% isoflurane in 100% O₂ gas) and warmed using heated air. A T1-weighted gradient-echo (GRE) 3D sequence (TR = 25 ms, TE_eff = 3, FOV = 64 mm, matrix = 128 × 128) was acquired during the FDG uptake period. Static PET images were acquired over 15 min in a 1-5 coincident in a single field of view with MRI range. Body temperature was maintained by flowing heated air on the animal bed (Multicell, Mediso, Hungary) and a pressure sensitive pad was used for respiratory triggering. PET images were reconstructed using Tera-Tomo 3D in full detector mode with all the corrections on high regularization and 8 iterations.

Kim A., Yu H.Y., Lim J., Ryu C.M., Kim Y.H., Heo J., Han J.Y., Lee S., Bae Y.S., Kim J.Y., Bae D.J., Kim S.Y., Noh B.J., Hong K.S., Han J.Y., Lee S.W., Song M., Chung H.M., Kim J.K., Shin D.M, & Choo M.S. (2017). Improved efficacy and in vivo cellular properties of human embryonic stem cell derivative in a preclinical model of bladder pain syndrome. Scientific Reports, 7, 8872.

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Multimodal Imaging Fusion Protocol

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Animals that were scanned on both PET and x-ray CT systems were placed on a custom-built platform in a rigid body fixed position (using 0.1-mm polyethylene wrapping) (20 (link)). The bed was placed into an integrated heated air, anesthesia bed (MultiCell, Mediso). The bed was fixed in place on the microPET gantry and imaged as above. The bed was then moved for CT imaging using the NanoSPECT/CT (Bioscan). General acquisition parameters were 55 kVp with a pitch of 1 and 240 projections in a spiral scan mode. The entire animal was scanned using a multiple-field-of-view procedure (with an approximate field of view of 4 cm × 4 cm × 4 cm per bed position), commonly requiring three bed positions per scan. Total scan time was about 10 min. A Shepp-Logan filter was used during the reconstruction process to produce image matrices with isotropic volumes of 221 μm.
PET data were reconstructed using a 3D-filtered back projection maximum a priori algorithm using a ramp filter with a cutoff frequency equal to the Nyquist frequency into a 128 × 128 × 95 matrix (50 (link)). Data were exported in raw format, and the rigid body (three degrees of freedom) coregistration between PET and CT data (and MRI, if applicable) was performed in Amira 5.3.3 (FEI). Amira and FIJI were used to produce most of the figures in the manuscript.

Thorek D.L., Watson P.A., Lee S.G., Ku A.T., Bournazos S., Braun K., Kim K., Sjöström K., Doran M.G., Lamminmäki U., Santos E., Veach D., Turkekul M., Casey E., Lewis J.S., Abou D.S., van Voss M.R., Scardino P.T., Strand S.E., Alpaugh M.L., Scher H.I., Lilja H., Larson S.M, & Ulmert D. (2016). Internalization of secreted antigen–targeted antibodies by the neonatal Fc receptor for precision imaging of the androgen receptor axis. Science translational medicine, 8(367), 367ra167.

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