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Total carbohydrate assay kit

Manufactured by Abcam
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The Total Carbohydrate Assay Kit is a colorimetric assay designed to quantify the total carbohydrate content in a variety of samples. The kit utilizes a phenol-sulfuric acid reaction to detect and measure carbohydrates, including monosaccharides, disaccharides, and polysaccharides.

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Lab products found in correlation

Lipid extraction kit, by Cell Biolabs (1 mentions) Strat m membrane, by Merck Group (1 mentions)

6 protocols using total carbohydrate assay kit

1

Purification and Quantification of Cryptococcal Capsular Antigens

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The capsular antigens were purified and concentrated by ultrafiltration using Vivacell 250 and ultrafiltration membrane with 100 kDa molecular weight cut-off as described by Nimrichter et al. (2007) [20 (link)]. Gelatinous like capsular antigens on the membrane were transferred to centrifuge tubes and kept at 4 °C. The total concentration of C. neoformans capsular antigens was quantified by phenol-sulfuric acid method (Total carbohydrate assay kit; Abcam) using D-glucose as the standard [16 (link)].
Sathongdejwisit P., Pruksaphon K., Intaramat A., Aiumurai P., Sookrung N., Ratanabanangkoon K., Nosanchuk J.D, & Youngchim S. (2021). A Novel, Inexpensive In-House Immunochromatographic Strip Test for Cryptococcosis Based on the Cryptococcal Glucuronoxylomannan Specific Monoclonal Antibody 18B7. Diagnostics, 11(5), 758.
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2

Comprehensive Fecal Lipid and Carbohydrate Analysis

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Fresh fecal pellets were collected and weighed. Fecal lipids were extracted using a Lipid Extraction Kit (Cell Biolabs STA-162). Extracted lipids were air dried, resuspended in butanol, and quantified using a Lipid Quantification Kit for neutral lipids (Cell BiolabsSTA-617). Fecal carbohydrates were quantified using a Total Carbohydrate Assay Kit (Abcam, ab155891) according to the manufacture’s protocol.
Greco C.M., Garetto S., Montellier E., Liu Y., Chen S., Baldi P., Sassone-Corsi P, & Lucci J. (2020). A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome. Scientific Reports, 10, 12915.
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3

Quantitative Analysis of Total Carbohydrates in Milk

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Total carbohydrate concentration in milk samples was measured using the Total Carbohydrate Assay Kit (Total Carbohydrate Assay Kit Quantification cat #155891, Abcam) following the manufacturer’s protocol. Whole milk samples were diluted at 1:500 in deionized water and 30 µL of each sample was transferred in duplicate into a 96-well plate. Absorbance was read on a microplate reader (Molecular Devices, SpectraMax Spectrofluorometers) at 490 nm.
Bradshaw C.V., Suarez Trujillo A., Luecke S.M., Logan L.D., Mohallem R., Aryal U.K., Stewart K.R., Casey T.M, & Minor R.C. (2021). Shotgun proteomics of homogenate milk reveals dynamic changes in protein abundances between colostrum, transitional, and mature milk of swine. Journal of Animal Science, 99(9), skab240.
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4

Cell-associated polysaccharide characterization

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Crude fractions of cell-associated polysaccharides (LPS plus capsule) were obtained from mid-log phase cultures of R99 strain in CM9 at 20, 25, 28, and 37°C as described by Hitchcock and Brown (1983) (link). Total polysaccharide concentration was determined with the Total Carbohydrate Assay Kit (BioVision) and 10 μg of cell-associated polysaccharides of each condition were separated by SDS-PAGE, transferred onto a PVDF membrane and subjected to immunoblot analyses according to Pajuelo et al. (2016) (link).
Hernández-Cabanyero C., Sanjuán E., Fouz B., Pajuelo D., Vallejos-Vidal E., Reyes-López F.E, & Amaro C. (2020). The Effect of the Environmental Temperature on the Adaptation to Host in the Zoonotic Pathogen Vibrio vulnificus. Frontiers in Microbiology, 11, 489.
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5

Quantifying N-Glycans via Carbohydrate Assay

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A total carbohydrate Assay Kit, (Biovision Milpitas, CA, USA), was used to quantify released N-glycans. To create a standard curve, 0, 2, 4, 6, 8, and 10 μL of mannose standard (2 mg/mL) were added into a series of wells in a 96-well microplate (Supporting information Figure S1). This generated 0, 4, 8, 12, 16, and 20 μg/well of mannose standard. The volume of each sample was adjusted to 30 μL per well with water. Thirty microliters of sample was used. One hundred and fifty microliters of concentrated sulfuric acid (98%) were added to each well. Samples were mixed on a shaker for ~1 min and then incubated at 85°C for 15 min. After incubation, 30 μL of Developer (provided by the manufacturer) was added to each well. Samples were again mixed on the shaker for 5 min. OD of each sample was measured at 490 nm. Sample OD was applied to the mannose standard curve linear function to calculate the quantity of carbohydrate in the sample.
Karav S., Bell J.M., Le Parc A., Liu Y., Mills D.A., Block D.E, & Barile D. (2015). Characterizing the Release of Bioactive N-Glycans from Dairy Products by a Novel Endo-β-N-Acetylglucosaminidase. Biotechnology progress, 31(5), 1331-1339.
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6

In Vitro Transdermal Diffusion of CME

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In vitro percutaneous absorption was measured using a manual diffusion system (PermeGear, Riegelsville, PA, USA) equipped with Strat-M membrane (Merck), which is a well-established synthetic model for transdermal diffusion testing. The 25 mm Strat-M membrane was mounted between the donor and receptor compartments and secured tightly with clamps. The available area of the membrane was 0.635 cm2. A 20 mg mL−1 solution of CME was loaded in the donor compartment, and the receptor compartment was filled with phosphate-buffered saline (PBS). The diffusion cells were placed on a magnetic stirring block, and the receptor compartment was maintained at 37 °C using a circulating water bath. Aliquots of 200 μL were withdrawn from the receptor compartment at various time points up to 48 h and analyzed using a total carbohydrate assay kit (Biovision) to determine the amount of CME that had permeated through the Strat-M membrane.
Lee M.C., Yeh H.Y, & Shih W.L. (2021). Extraction Procedure, Characteristics, and Feasibility of Caulerpa microphysa (Chlorophyta) Polysaccharide Extract as a Cosmetic Ingredient. Marine Drugs, 19(9), 524.
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