The SMO antibody was raised in rabbits (Pocono Rabbit Farm and Laboratory Inc.) using antigens and procedures described in Rohatgi et. al. [43 (link)]; and used at a dilution of 1:500. The KIF7 antibody [41 (link)] was used at 1:1000. Monoclonal antibodies against NKX2.2 and ISL1 were used at 1:10 and obtained from the Developmental Studies Hybridoma Bank. Commercially available antibodies used were: rabbit α-FOXA2 (Millipore, 1:500) rabbit α-OLIG2 (1:200, Millipore), mouse α-acetylated α-Tubulin (1:5000, Sigma Aldrich), mouse α- γ- Tubulin (1:5000, Sigma Aldrich), rabbit α-IFT88 (1:500, Proteintech), rabbit α-IFT81 (1:200, Proteintech), INPP5E (1:200, Proteintech), GPR161 (1:100, Proteintech). The GLI2 [44 (link)] and ARL13B [45 (link)] antibodies were described previously.
Rabbit α ift88
Manufactured by Proteintech
Sourced in Belgium
Rabbit α-IFT88 is an antibody product designed for the detection of IFT88 protein in various applications. IFT88 is a component of the intraflagellar transport complex and plays a crucial role in cilia formation and function.
Automatically generated - may contain errors
2 protocols using rabbit α ift88
1
Antibody Use for Cell Signaling Analysis
2
Antibody Panel for Ciliary Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies used were mouse α-acetylated tubulin 6-11B-1 (TubAc; Sigma-Aldrich), rabbit α-Tmem231 (Sigma-Aldrich), rabbit α-Mks1 (Proteintech), rabbit α-Tmem67/Mks3 (Proteintech), rabbit α-Rpgrip1l (Proteintech), rabbit α-Nphp1 (gift of G. Pazour, University of Massachusetts Medical School, Worcester, MA; amino acids 1–209 [Benzing et al., 2001 (link); Fliegauf et al., 2006 (link)]), rabbit α-Arl13b (Proteintech), mouse α-Arl13b (Proteintech), rabbit α-INPP5e (gift of S. Schurmans, Université de Liège, Liège, Belgium; antigen: N-terminal MLQGQPPNTEKKLIP peptide [Jacoby et al., 2009 (link)]), rabbit α-Adcy3 (Proteintech), rabbit α-IFT88 (Proteintech), rabbit α-Pkd2 (YCC; gift of S. Somlo, Yale School of Medicine, New Haven, CT; antigen: amino acids 687–962 [Wu et al., 1998 (link)]), rabbit goat α–γ-tubulin (Santa Cruz Biotechnology, Inc.), goat α-V5 (Abcam), and mouse α-FLAG-M2 (Sigma-Aldrich). Rabbit α-B9d1 antibody was generated by injecting a full-length mouse B9d1 protein fused to a His tag into rabbits (Pacific Immunology) and affinity purifying the antibody with recombinant B9d1-6×His. Secondary antibodies are as follows: donkey α-mouse 647, donkey α-mouse 488, donkey α-rabbit 488, donkey α-rabbit 647, and donkey α-goat 568 (Alexa Fluor).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!