5 aza cdr

H9c2 rat cardiomyocyte cell line was get from Wuhan Punosi Life Science and Technology Co., Ltd (Wuhan, China). DMEM low-sugar medium and FBS was purchased from HyClone (Logan, UT). The CCK-8 was purchased from Dojindo (Kumamoto, Japan). LDH, CK-MB, ROS, and GSH test kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Iron assay kit and GPX4 primary antibodies were purchased from Abcam (Cambridge, USA). Rabbit anti-rat primary antibodies DNMT-1 (catalog number 24206–1-AP), GPX4 (catalog number 67763-1-Ig), P62 (catalog number 18420-1-AP), Beclin-1 (catalog number 11306-1-AP), GAPDH (catalog number 60004-1-Ig), and Cy3- (catalog number SA00009-2) and FITC- (catalog number SA00003-2)-labeled goat anti-rabbit secondary antibodies were obtained from ProteinTech (Wuhan, China). Rabbit anti-rat primary antibodies NCOA4 (catalog number A5695) and FTH (catalog number A19544) were obtained from ABclonal (Wuhan, China). Second antibodies were purchased from LI-COR Biosciences (IRDye 800CW; catalog number 926-32219, LI-COR Corporate, USA). DNMT-1 inhibitor 5-aza-CdR were purchased from Selleck (Houston, USA). Lipofectamine 2000 was obtained from Invitrogen (San Diego, USA).

293T, PLC, HepG2 and Hepa1-6 cells were obtained from American Type Culture Collection (ATCC) and cultured in Dulbecco’s modified Eagle’s medium (Hyclone) supplemented with 10% fetal bovine serum (FBS, Zeta Life), 100 units/ml penicillin and 100 μg/ml streptomycin (Beyotime Bio-technology). Hep3B was cultured in minimal essential medium (Procell). All cells were cultured at 37 °C in a humidified incubator with 5% CO₂. Polyethylen imine (PEI, Polysciences), 5­Aza­CdR, Puromycin (Selleck), Lipo8000, polybrene (Beyotime Bio-technology) were obtained from the companies listed above.