Blood was collected in the fasting state from the aorta artery after rats were sacrificed. Glucose concentrations were determined by using Konelab 20 (Thermo Electron Corporation) and Konelab system reagents (Thermo Fisher Scientific, Vantaa, Finland). Plasma insulin concentrations were measured using an ELISA kit (Millipore Corporate Headquarters, Billerica, MA, United States). The fasting glucose-to-insulin ratio (FGIR) was calculated (Bligh and Dyer, 1959 (link); Cacho et al., 2008 (link); Bowe et al., 2014 (link)).
Konelab system reagents
Manufactured by Thermo Fisher Scientific
Sourced in Finland
The Konelab system reagents are a set of chemical solutions used in automated clinical chemistry analyzers manufactured by Thermo Fisher Scientific. These reagents are designed to work with the Konelab series of instruments and are used for the quantitative determination of various analytes in biological samples, such as blood, urine, or other bodily fluids.
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2 protocols using konelab system reagents
1
Fasting Glucose and Insulin Analysis
2
Plasma Metabolite Measurement Protocol
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The samples for plasma glucose measurements were collected in citrate-fluoride tubes and for plasma insulin and non-esterified fatty acids (NEFA) in EDTA tubes kept on ice. Plasma was immediately separated by centrifugation at þ4 C and stored at À70 C.
Glucose concentrations were analyzed with the hexokinase method using Konelab System reagents and Konelab 20 XTi clinical chemistry analyzer (Thermo Fisher Scientific, Vantaa, Finland). Insulin was measured with a chemiluminometric immunoassay using Liaison® Insulin reagents (DiaSorin Inc, Stillwater, MN, USA) and DiaSorin Liaison® analyser (DiaSorin Deutchland GmbH, Dietzenbach, Germany). NEFA concentrations were measured with a enzymatic colorimetric method using Wako NEFA-HR(2) reagents (Wako Chemicals GmbH, Neuss, Germany) and Konelab 20 XTi analyzer.
Glucose concentrations were analyzed with the hexokinase method using Konelab System reagents and Konelab 20 XTi clinical chemistry analyzer (Thermo Fisher Scientific, Vantaa, Finland). Insulin was measured with a chemiluminometric immunoassay using Liaison® Insulin reagents (DiaSorin Inc, Stillwater, MN, USA) and DiaSorin Liaison® analyser (DiaSorin Deutchland GmbH, Dietzenbach, Germany). NEFA concentrations were measured with a enzymatic colorimetric method using Wako NEFA-HR(2) reagents (Wako Chemicals GmbH, Neuss, Germany) and Konelab 20 XTi analyzer.
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